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Manganese oxide functionalized silk fibers for enzyme mimics application

Singh, Manish LU ; Dey, Estera S. LU and Dicko, Cedric LU orcid (2020) In Reactive and Functional Polymers
Abstract
The inorganic metal or metal-oxide nanoparticles (NPs) that mimic enzymes are of great interest due to improved physical and chemical properties compared with native enzymes. Here, we report that manganese dioxide (MnO2)-Silk exhibit catalase, oxidase, and peroxidase-like activities. The MnO2-Silk hybrid fibers effectively decomposed hydrogen peroxide (H2O2) and oxidized the typical horseradish peroxidase substrates, such as o-phenylenediamine (OPD), and 3,3′,5,5′- tetramethylbenzidine (TMB) in the presence or absence of H2O2. The oxidative properties of MnO2-Silk fiber hybrid showed an enzyme-like behavior for the catalase-like activity, oxidase-like activity, and peroxidase-like activity. The operational stability of the MnO2-Silk fiber... (More)
The inorganic metal or metal-oxide nanoparticles (NPs) that mimic enzymes are of great interest due to improved physical and chemical properties compared with native enzymes. Here, we report that manganese dioxide (MnO2)-Silk exhibit catalase, oxidase, and peroxidase-like activities. The MnO2-Silk hybrid fibers effectively decomposed hydrogen peroxide (H2O2) and oxidized the typical horseradish peroxidase substrates, such as o-phenylenediamine (OPD), and 3,3′,5,5′- tetramethylbenzidine (TMB) in the presence or absence of H2O2. The oxidative properties of MnO2-Silk fiber hybrid showed an enzyme-like behavior for the catalase-like activity, oxidase-like activity, and peroxidase-like activity. The operational stability of the MnO2-Silk fiber hybrid over ten cycles showed a constant residual activity of about 25–30% after 2–3 cycles indicating that MnO2-Silk fiber hybrid could be used as a satisfactory oxidoreductase enzyme mimics. Potentiometric titration was used to determine the surface charges of the MnO2-Silk catalyst. Together, we identified the reactive species as Mn1−x4+Mnx3+O2−x(OH)x with a pK of approximately 5.2. Our results have implications on the understanding of the catalytic origin and interaction of metal oxides NP with various biomaterials. (Less)
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author
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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Enzyme-mimic, Catalase, Oxidase, Peroxidase, Functionalized silk, Potentiometric titration, pK distribution
in
Reactive and Functional Polymers
article number
104565
publisher
Elsevier
external identifiers
  • scopus:85082393489
ISSN
1381-5148
DOI
10.1016/j.reactfunctpolym.2020.104565
language
English
LU publication?
yes
id
b9351495-dcc1-4f25-9712-d5f6761023c1
date added to LUP
2020-03-13 16:53:24
date last changed
2022-04-18 21:19:00
@article{b9351495-dcc1-4f25-9712-d5f6761023c1,
  abstract     = {{The inorganic metal or metal-oxide nanoparticles (NPs) that mimic enzymes are of great interest due to improved physical and chemical properties compared with native enzymes. Here, we report that manganese dioxide (MnO2)-Silk exhibit catalase, oxidase, and peroxidase-like activities. The MnO2-Silk hybrid fibers effectively decomposed hydrogen peroxide (H2O2) and oxidized the typical horseradish peroxidase substrates, such as o-phenylenediamine (OPD), and 3,3′,5,5′- tetramethylbenzidine (TMB) in the presence or absence of H2O2. The oxidative properties of MnO2-Silk fiber hybrid showed an enzyme-like behavior for the catalase-like activity, oxidase-like activity, and peroxidase-like activity. The operational stability of the MnO2-Silk fiber hybrid over ten cycles showed a constant residual activity of about 25–30% after 2–3 cycles indicating that MnO2-Silk fiber hybrid could be used as a satisfactory oxidoreductase enzyme mimics. Potentiometric titration was used to determine the surface charges of the MnO2-Silk catalyst. Together, we identified the reactive species as Mn1−x4+Mnx3+O2−x(OH)x with a pK of approximately 5.2. Our results have implications on the understanding of the catalytic origin and interaction of metal oxides NP with various biomaterials.}},
  author       = {{Singh, Manish and Dey, Estera S. and Dicko, Cedric}},
  issn         = {{1381-5148}},
  keywords     = {{Enzyme-mimic; Catalase; Oxidase; Peroxidase; Functionalized silk; Potentiometric titration; pK distribution}},
  language     = {{eng}},
  publisher    = {{Elsevier}},
  series       = {{Reactive and Functional Polymers}},
  title        = {{Manganese oxide functionalized silk fibers for enzyme mimics application}},
  url          = {{http://dx.doi.org/10.1016/j.reactfunctpolym.2020.104565}},
  doi          = {{10.1016/j.reactfunctpolym.2020.104565}},
  year         = {{2020}},
}