Quantification of Plasma Kynurenine Metabolites Following One Bout of Sprint Interval Exercise
(2020) In International Journal of Tryptophan Research 13.- Abstract
The kynurenine pathway of tryptophan degradation produces several neuroactive metabolites suggested to be involved in a wide variety of diseases and disorders, however, technical challenges in reliably detecting these metabolites hampers cross-comparisons. The main objective of this study was to develop an accurate, robust and precise bioanalytical method for simultaneous quantification of ten plasma kynurenine metabolites. As a secondary aim, we applied this method on blood samples taken from healthy subjects conducting 1 session of sprint interval exercise (SIE). It is well accepted that physical exercise is associated with health benefits and reduces risks of psychiatric illness, diabetes, cancer and cardiovascular disease, but also... (More)
The kynurenine pathway of tryptophan degradation produces several neuroactive metabolites suggested to be involved in a wide variety of diseases and disorders, however, technical challenges in reliably detecting these metabolites hampers cross-comparisons. The main objective of this study was to develop an accurate, robust and precise bioanalytical method for simultaneous quantification of ten plasma kynurenine metabolites. As a secondary aim, we applied this method on blood samples taken from healthy subjects conducting 1 session of sprint interval exercise (SIE). It is well accepted that physical exercise is associated with health benefits and reduces risks of psychiatric illness, diabetes, cancer and cardiovascular disease, but also influences the peripheral and central concentrations of kynurenines. In line with this, we found that in healthy old adults (n = 10; mean age 64 years), levels of kynurenine increased 1 hour (P =.03) after SIE, while kynurenic acid (KYNA) concentrations were elevated after 24 hours (P =.02). In contrast, no significant changes after exercise were seen in young adults (n = 10; mean age 24 years). In conclusion, the described method performs well in reliably detecting all the analyzed metabolites in plasma samples. Furthermore, we also detected an age-dependent effect on the degree by which a single intense training session affects kynurenine metabolite levels.
(Less)
- author
- organization
- publishing date
- 2020
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- kynurenic acid, Kynurenine, LC/MS/MS, sprint interval exercise, tryptophan-kynurenine pathway
- in
- International Journal of Tryptophan Research
- volume
- 13
- publisher
- Libertas Academica
- external identifiers
-
- scopus:85097422753
- pmid:33354112
- ISSN
- 1178-6469
- DOI
- 10.1177/1178646920978241
- language
- English
- LU publication?
- yes
- id
- b9d7796e-ab0e-4ee8-9a2b-d8910d179dae
- date added to LUP
- 2020-12-23 09:10:39
- date last changed
- 2024-06-14 05:37:51
@article{b9d7796e-ab0e-4ee8-9a2b-d8910d179dae,
abstract = {{<p>The kynurenine pathway of tryptophan degradation produces several neuroactive metabolites suggested to be involved in a wide variety of diseases and disorders, however, technical challenges in reliably detecting these metabolites hampers cross-comparisons. The main objective of this study was to develop an accurate, robust and precise bioanalytical method for simultaneous quantification of ten plasma kynurenine metabolites. As a secondary aim, we applied this method on blood samples taken from healthy subjects conducting 1 session of sprint interval exercise (SIE). It is well accepted that physical exercise is associated with health benefits and reduces risks of psychiatric illness, diabetes, cancer and cardiovascular disease, but also influences the peripheral and central concentrations of kynurenines. In line with this, we found that in healthy old adults (n = 10; mean age 64 years), levels of kynurenine increased 1 hour (P =.03) after SIE, while kynurenic acid (KYNA) concentrations were elevated after 24 hours (P =.02). In contrast, no significant changes after exercise were seen in young adults (n = 10; mean age 24 years). In conclusion, the described method performs well in reliably detecting all the analyzed metabolites in plasma samples. Furthermore, we also detected an age-dependent effect on the degree by which a single intense training session affects kynurenine metabolite levels.</p>}},
author = {{Trepci, Ada and Imbeault, Sophie and Wyckelsma, Victoria L. and Westerblad, Håkan and Hermansson, Sigurd and Andersson, Daniel C. and Piehl, Fredrik and Venckunas, Tomas and Brazaitis, Marius and Kamandulis, Sigitas and Brundin, Lena and Erhardt, Sophie and Schwieler, Lilly}},
issn = {{1178-6469}},
keywords = {{kynurenic acid; Kynurenine; LC/MS/MS; sprint interval exercise; tryptophan-kynurenine pathway}},
language = {{eng}},
publisher = {{Libertas Academica}},
series = {{International Journal of Tryptophan Research}},
title = {{Quantification of Plasma Kynurenine Metabolites Following One Bout of Sprint Interval Exercise}},
url = {{http://dx.doi.org/10.1177/1178646920978241}},
doi = {{10.1177/1178646920978241}},
volume = {{13}},
year = {{2020}},
}