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Bifidobacterium mellis sp. nov., isolated from the honey stomach of the honey bee Apis mellifera

Olofsson, Tobias C. LU ; Modesto, Monica ; Pascarelli, Stefano ; Scarafile, Donatella ; Mattarelli, Paola and Vasquez, Alejandra LU (2023) In International Journal of Systematic and Evolutionary Microbiology 73(3).
Abstract

A novel Bifidobacterium strain, Bin7NT, was isolated from the honey stomach of the honey bee Apis mellifera. Cells are Gram-positive, non-motile, non-sporulating, facultative anaerobic and fructose 6-phosphate phosphoketolase-positive. Their optimal growth is at 37 °C in anaerobiosis in MRS (De Man, Rogosa and Sharpe) added with cysteine. The honey bee microbiota was composed of several phylotypes of Bifidobacterium and Lactobacillus. Comparative analysis of 16S rRNA gene sequence similarity revealed that strain Bin7NT grouped with Bifidobacterium species originating from honey bees and was closely related to Bifidobacterium asteroides DSM 20089T (99.67 % similarity). However, the highest average... (More)

A novel Bifidobacterium strain, Bin7NT, was isolated from the honey stomach of the honey bee Apis mellifera. Cells are Gram-positive, non-motile, non-sporulating, facultative anaerobic and fructose 6-phosphate phosphoketolase-positive. Their optimal growth is at 37 °C in anaerobiosis in MRS (De Man, Rogosa and Sharpe) added with cysteine. The honey bee microbiota was composed of several phylotypes of Bifidobacterium and Lactobacillus. Comparative analysis of 16S rRNA gene sequence similarity revealed that strain Bin7NT grouped with Bifidobacterium species originating from honey bees and was closely related to Bifidobacterium asteroides DSM 20089T (99.67 % similarity). However, the highest average nucleotide identity and digital DNA– DNA hybridization values of 94.88 and 60.6 %, respectively, were obtained with Bifidobacterium choladohabitans JCM 34586T. The DNA G+C content of the type strain is 60.8 mol%. The cell-wall peptidoglycan is of the A4β l-Orn–d-Asp type. The main cellular fatty acids of strain Bin7NT are C18: 1 ω9c, C16: 0, C18: 1 ω7c and C18: 0. Phenotypic characterization and genotyping based on the genome sequences clearly show that this strain is distinct from the type strains of the so far recognized Bifidobacterium species. Thus, Bifidobacterium mellis sp. nov. (Bin7NT=DSM 29108T=CCUG 66113T) is proposed as novel Bifidobacterium species.

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author
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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Apis mellifera, Bifidobacterium, Bifidobacterium mellis sp. nov., honey stomach, new species
in
International Journal of Systematic and Evolutionary Microbiology
volume
73
issue
3
article number
005766
publisher
Microbiology Society
external identifiers
  • scopus:85149360822
  • pmid:36884368
ISSN
1466-5026
DOI
10.1099/ijsem.0.005766
language
English
LU publication?
yes
id
ba6eb336-638c-42ca-839a-f5a6b96a8d11
date added to LUP
2024-01-12 14:35:55
date last changed
2024-04-27 09:58:39
@article{ba6eb336-638c-42ca-839a-f5a6b96a8d11,
  abstract     = {{<p>A novel Bifidobacterium strain, Bin7N<sup>T</sup>, was isolated from the honey stomach of the honey bee Apis mellifera. Cells are Gram-positive, non-motile, non-sporulating, facultative anaerobic and fructose 6-phosphate phosphoketolase-positive. Their optimal growth is at 37 °C in anaerobiosis in MRS (De Man, Rogosa and Sharpe) added with cysteine. The honey bee microbiota was composed of several phylotypes of Bifidobacterium and Lactobacillus. Comparative analysis of 16S rRNA gene sequence similarity revealed that strain Bin7N<sup>T</sup> grouped with Bifidobacterium species originating from honey bees and was closely related to Bifidobacterium asteroides DSM 20089<sup>T</sup> (99.67 % similarity). However, the highest average nucleotide identity and digital DNA– DNA hybridization values of 94.88 and 60.6 %, respectively, were obtained with Bifidobacterium choladohabitans JCM 34586<sup>T</sup>. The DNA G+C content of the type strain is 60.8 mol%. The cell-wall peptidoglycan is of the A4β l-Orn–d-Asp type. The main cellular fatty acids of strain Bin7N<sup>T</sup> are C<sub>18: 1</sub> ω9c, C<sub>16: 0</sub>, C<sub>18: 1</sub> ω7c and C<sub>18: 0</sub>. Phenotypic characterization and genotyping based on the genome sequences clearly show that this strain is distinct from the type strains of the so far recognized Bifidobacterium species. Thus, Bifidobacterium mellis sp. nov. (Bin7N<sup>T</sup>=DSM 29108<sup>T</sup>=CCUG 66113<sup>T</sup>) is proposed as novel Bifidobacterium species.</p>}},
  author       = {{Olofsson, Tobias C. and Modesto, Monica and Pascarelli, Stefano and Scarafile, Donatella and Mattarelli, Paola and Vasquez, Alejandra}},
  issn         = {{1466-5026}},
  keywords     = {{Apis mellifera; Bifidobacterium; Bifidobacterium mellis sp. nov.; honey stomach; new species}},
  language     = {{eng}},
  number       = {{3}},
  publisher    = {{Microbiology Society}},
  series       = {{International Journal of Systematic and Evolutionary Microbiology}},
  title        = {{Bifidobacterium mellis sp. nov., isolated from the honey stomach of the honey bee Apis mellifera}},
  url          = {{http://dx.doi.org/10.1099/ijsem.0.005766}},
  doi          = {{10.1099/ijsem.0.005766}},
  volume       = {{73}},
  year         = {{2023}},
}