High throughput system for magnetic manipulation of cells, polymers, and biomaterials
(2008) In Review of Scientific Instruments 79(8).- Abstract
In the past decade, high throughput screening (HTS) has changed the way biochemical assays are performed, but manipulation and mechanical measurement of micro- and nanoscale systems have not benefited from this trend. Techniques using microbeads (particles approximately 0.1-10 mum) show promise for enabling high throughput mechanical measurements of microscopic systems. We demonstrate instrumentation to magnetically drive microbeads in a biocompatible, multiwell magnetic force system. It is based on commercial HTS standards and is scalable to 96 wells. Cells can be cultured in this magnetic high throughput system (MHTS). The MHTS can apply independently controlled forces to 16 specimen wells. Force calibrations demonstrate forces in... (More)
In the past decade, high throughput screening (HTS) has changed the way biochemical assays are performed, but manipulation and mechanical measurement of micro- and nanoscale systems have not benefited from this trend. Techniques using microbeads (particles approximately 0.1-10 mum) show promise for enabling high throughput mechanical measurements of microscopic systems. We demonstrate instrumentation to magnetically drive microbeads in a biocompatible, multiwell magnetic force system. It is based on commercial HTS standards and is scalable to 96 wells. Cells can be cultured in this magnetic high throughput system (MHTS). The MHTS can apply independently controlled forces to 16 specimen wells. Force calibrations demonstrate forces in excess of 1 nN, predicted force saturation as a function of pole material, and powerlaw dependence of F approximately r(-2.7+/-0.1). We employ this system to measure the stiffness of SR2+ Drosophila cells. MHTS technology is a key step toward a high throughput screening system for micro- and nanoscale biophysical experiments.
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- author
- Spero, Richard Chasen ; Vicci, Leandra ; Cribb, Jeremy ; Bober, David ; Swaminathan, Vinay LU ; O'Brien, E Timothy ; Rogers, Stephen L and Superfine, R
- publishing date
- 2008-08
- type
- Contribution to journal
- publication status
- published
- keywords
- Animals, Biocompatible Materials/chemistry, Calibration, Cells/cytology, Cells, Cultured, Drosophila/cytology, Equipment Design, Magnetics/instrumentation, Micromanipulation/instrumentation, Microscopy, Video, Microspheres, Miniaturization, Physical Phenomena, Polymers/chemistry, Temperature
- in
- Review of Scientific Instruments
- volume
- 79
- issue
- 8
- article number
- 083707
- publisher
- American Institute of Physics (AIP)
- external identifiers
-
- pmid:19044357
- scopus:50849126777
- ISSN
- 1089-7623
- DOI
- 10.1063/1.2976156
- language
- English
- LU publication?
- no
- id
- ba9ed126-49c5-46af-8b1a-db65d3cdd071
- date added to LUP
- 2018-09-07 16:52:23
- date last changed
- 2024-01-15 01:06:10
@article{ba9ed126-49c5-46af-8b1a-db65d3cdd071, abstract = {{<p>In the past decade, high throughput screening (HTS) has changed the way biochemical assays are performed, but manipulation and mechanical measurement of micro- and nanoscale systems have not benefited from this trend. Techniques using microbeads (particles approximately 0.1-10 mum) show promise for enabling high throughput mechanical measurements of microscopic systems. We demonstrate instrumentation to magnetically drive microbeads in a biocompatible, multiwell magnetic force system. It is based on commercial HTS standards and is scalable to 96 wells. Cells can be cultured in this magnetic high throughput system (MHTS). The MHTS can apply independently controlled forces to 16 specimen wells. Force calibrations demonstrate forces in excess of 1 nN, predicted force saturation as a function of pole material, and powerlaw dependence of F approximately r(-2.7+/-0.1). We employ this system to measure the stiffness of SR2+ Drosophila cells. MHTS technology is a key step toward a high throughput screening system for micro- and nanoscale biophysical experiments.</p>}}, author = {{Spero, Richard Chasen and Vicci, Leandra and Cribb, Jeremy and Bober, David and Swaminathan, Vinay and O'Brien, E Timothy and Rogers, Stephen L and Superfine, R}}, issn = {{1089-7623}}, keywords = {{Animals; Biocompatible Materials/chemistry; Calibration; Cells/cytology; Cells, Cultured; Drosophila/cytology; Equipment Design; Magnetics/instrumentation; Micromanipulation/instrumentation; Microscopy, Video; Microspheres; Miniaturization; Physical Phenomena; Polymers/chemistry; Temperature}}, language = {{eng}}, number = {{8}}, publisher = {{American Institute of Physics (AIP)}}, series = {{Review of Scientific Instruments}}, title = {{High throughput system for magnetic manipulation of cells, polymers, and biomaterials}}, url = {{http://dx.doi.org/10.1063/1.2976156}}, doi = {{10.1063/1.2976156}}, volume = {{79}}, year = {{2008}}, }