Advanced

High throughput system for magnetic manipulation of cells, polymers, and biomaterials

Spero, Richard Chasen; Vicci, Leandra; Cribb, Jeremy; Bober, David; Swaminathan, Vinay LU ; O'Brien, E Timothy; Rogers, Stephen L and Superfine, R (2008) In The Review of Scientific Instruments 79(8).
Abstract

In the past decade, high throughput screening (HTS) has changed the way biochemical assays are performed, but manipulation and mechanical measurement of micro- and nanoscale systems have not benefited from this trend. Techniques using microbeads (particles approximately 0.1-10 mum) show promise for enabling high throughput mechanical measurements of microscopic systems. We demonstrate instrumentation to magnetically drive microbeads in a biocompatible, multiwell magnetic force system. It is based on commercial HTS standards and is scalable to 96 wells. Cells can be cultured in this magnetic high throughput system (MHTS). The MHTS can apply independently controlled forces to 16 specimen wells. Force calibrations demonstrate forces in... (More)

In the past decade, high throughput screening (HTS) has changed the way biochemical assays are performed, but manipulation and mechanical measurement of micro- and nanoscale systems have not benefited from this trend. Techniques using microbeads (particles approximately 0.1-10 mum) show promise for enabling high throughput mechanical measurements of microscopic systems. We demonstrate instrumentation to magnetically drive microbeads in a biocompatible, multiwell magnetic force system. It is based on commercial HTS standards and is scalable to 96 wells. Cells can be cultured in this magnetic high throughput system (MHTS). The MHTS can apply independently controlled forces to 16 specimen wells. Force calibrations demonstrate forces in excess of 1 nN, predicted force saturation as a function of pole material, and powerlaw dependence of F approximately r(-2.7+/-0.1). We employ this system to measure the stiffness of SR2+ Drosophila cells. MHTS technology is a key step toward a high throughput screening system for micro- and nanoscale biophysical experiments.

(Less)
Please use this url to cite or link to this publication:
author
publishing date
type
Contribution to journal
publication status
published
keywords
Animals, Biocompatible Materials/chemistry, Calibration, Cells/cytology, Cells, Cultured, Drosophila/cytology, Equipment Design, Magnetics/instrumentation, Micromanipulation/instrumentation, Microscopy, Video, Microspheres, Miniaturization, Physical Phenomena, Polymers/chemistry, Temperature
in
The Review of Scientific Instruments
volume
79
issue
8
publisher
American Institute of Physics
external identifiers
  • scopus:50849126777
ISSN
1089-7623
DOI
10.1063/1.2976156
language
English
LU publication?
no
id
ba9ed126-49c5-46af-8b1a-db65d3cdd071
date added to LUP
2018-09-07 16:52:23
date last changed
2018-09-16 04:57:02
@article{ba9ed126-49c5-46af-8b1a-db65d3cdd071,
  abstract     = {<p>In the past decade, high throughput screening (HTS) has changed the way biochemical assays are performed, but manipulation and mechanical measurement of micro- and nanoscale systems have not benefited from this trend. Techniques using microbeads (particles approximately 0.1-10 mum) show promise for enabling high throughput mechanical measurements of microscopic systems. We demonstrate instrumentation to magnetically drive microbeads in a biocompatible, multiwell magnetic force system. It is based on commercial HTS standards and is scalable to 96 wells. Cells can be cultured in this magnetic high throughput system (MHTS). The MHTS can apply independently controlled forces to 16 specimen wells. Force calibrations demonstrate forces in excess of 1 nN, predicted force saturation as a function of pole material, and powerlaw dependence of F approximately r(-2.7+/-0.1). We employ this system to measure the stiffness of SR2+ Drosophila cells. MHTS technology is a key step toward a high throughput screening system for micro- and nanoscale biophysical experiments.</p>},
  articleno    = {083707},
  author       = {Spero, Richard Chasen and Vicci, Leandra and Cribb, Jeremy and Bober, David and Swaminathan, Vinay and O'Brien, E Timothy and Rogers, Stephen L and Superfine, R},
  issn         = {1089-7623},
  keyword      = {Animals,Biocompatible Materials/chemistry,Calibration,Cells/cytology,Cells, Cultured,Drosophila/cytology,Equipment Design,Magnetics/instrumentation,Micromanipulation/instrumentation,Microscopy, Video,Microspheres,Miniaturization,Physical Phenomena,Polymers/chemistry,Temperature},
  language     = {eng},
  number       = {8},
  publisher    = {American Institute of Physics},
  series       = {The Review of Scientific Instruments},
  title        = {High throughput system for magnetic manipulation of cells, polymers, and biomaterials},
  url          = {http://dx.doi.org/10.1063/1.2976156},
  volume       = {79},
  year         = {2008},
}