Mesh bags underestimated arbuscular mycorrhizal abundance but captured fertilization effects in a mesocosm experiment
Ven, Arne LU ; Verbruggen, Erik ; Verlinden, Melanie S. ; Olsson, Pål Axel LU ; Wallander, Håkan LU
and Vicca, Sara
(2020)
In Plant and Soil
446(1-2).
p.563-575
- Abstract
Aims: Ingrowth bags are widely used to estimate mycorrhizal growth and dynamics. However, it remains unclear to what extent they reflect the surrounding soil, and how this varies with environmental conditions. Methods: We used a fertilization experiment to investigate if carbon-free mesh bags were representative of their surrounding soil. We determined AMF hyphal length density (HLD), phospholipid fatty acids (PLFA 16:1ω5) and neutral lipid fatty acids (NLFA 16:1ω5). Results: When AMF abundance in surrounding soil was high, HLD and both fatty acids were underestimated by the mesh bags. The PLFA 16:1ω5 in surrounding soil included bacterial PLFA, complicating the comparison of PLFA 16:1ω5 between mesh bags and surrounding soil. Both NLFA... (More)
Aims: Ingrowth bags are widely used to estimate mycorrhizal growth and dynamics. However, it remains unclear to what extent they reflect the surrounding soil, and how this varies with environmental conditions. Methods: We used a fertilization experiment to investigate if carbon-free mesh bags were representative of their surrounding soil. We determined AMF hyphal length density (HLD), phospholipid fatty acids (PLFA 16:1ω5) and neutral lipid fatty acids (NLFA 16:1ω5). Results: When AMF abundance in surrounding soil was high, HLD and both fatty acids were underestimated by the mesh bags. The PLFA 16:1ω5 in surrounding soil included bacterial PLFA, complicating the comparison of PLFA 16:1ω5 between mesh bags and surrounding soil. Both NLFA 16:1ω5 and HLD showed a significantly positive correlation, and fertilization effects were mostly similar for mesh bags and surrounding soil. Conclusions: Although carbon-free mesh bags can underestimate AMF abundance in soils, they represent a useful method to compare patterns in AMF abundance across environmental gradients and can be particularly useful in combination with the use of stable isotope tracers for unraveling patterns in AMF growth. NLFA 16:1ω5 appeared a more accurate measure for AMF than PLFA 16:1ω5 because the latter included bacterial PLFA.
(Less)
- author
- Ven, Arne
LU
; Verbruggen, Erik
; Verlinden, Melanie S.
; Olsson, Pål Axel
LU
; Wallander, Håkan
LU
and Vicca, Sara
- organization
- publishing date
- 2020
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Hyphal length density (HLD), Mycorrhizal fungi, Neutral lipid fatty acid (NLFA), Phospholipid fatty acid (PLFA), Stable carbon isotopes
- in
- Plant and Soil
- volume
- 446
- issue
- 1-2
- pages
- 13 pages
- publisher
- Springer
- external identifiers
-
- scopus:85075901258
- ISSN
- 0032-079X
- DOI
- 10.1007/s11104-019-04368-4
- language
- English
- LU publication?
- yes
- id
- baa136bb-dc93-4fff-ab35-dcd64cbfbca5
- date added to LUP
- 2021-01-04 11:00:15
- date last changed
- 2022-04-26 22:58:05
@article{baa136bb-dc93-4fff-ab35-dcd64cbfbca5,
abstract = {{<p>Aims: Ingrowth bags are widely used to estimate mycorrhizal growth and dynamics. However, it remains unclear to what extent they reflect the surrounding soil, and how this varies with environmental conditions. Methods: We used a fertilization experiment to investigate if carbon-free mesh bags were representative of their surrounding soil. We determined AMF hyphal length density (HLD), phospholipid fatty acids (PLFA 16:1ω5) and neutral lipid fatty acids (NLFA 16:1ω5). Results: When AMF abundance in surrounding soil was high, HLD and both fatty acids were underestimated by the mesh bags. The PLFA 16:1ω5 in surrounding soil included bacterial PLFA, complicating the comparison of PLFA 16:1ω5 between mesh bags and surrounding soil. Both NLFA 16:1ω5 and HLD showed a significantly positive correlation, and fertilization effects were mostly similar for mesh bags and surrounding soil. Conclusions: Although carbon-free mesh bags can underestimate AMF abundance in soils, they represent a useful method to compare patterns in AMF abundance across environmental gradients and can be particularly useful in combination with the use of stable isotope tracers for unraveling patterns in AMF growth. NLFA 16:1ω5 appeared a more accurate measure for AMF than PLFA 16:1ω5 because the latter included bacterial PLFA.</p>}},
author = {{Ven, Arne and Verbruggen, Erik and Verlinden, Melanie S. and Olsson, Pål Axel and Wallander, Håkan and Vicca, Sara}},
issn = {{0032-079X}},
keywords = {{Hyphal length density (HLD); Mycorrhizal fungi; Neutral lipid fatty acid (NLFA); Phospholipid fatty acid (PLFA); Stable carbon isotopes}},
language = {{eng}},
number = {{1-2}},
pages = {{563--575}},
publisher = {{Springer}},
series = {{Plant and Soil}},
title = {{Mesh bags underestimated arbuscular mycorrhizal abundance but captured fertilization effects in a mesocosm experiment}},
url = {{http://dx.doi.org/10.1007/s11104-019-04368-4}},
doi = {{10.1007/s11104-019-04368-4}},
volume = {{446}},
year = {{2020}},
}