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Mesh bags underestimated arbuscular mycorrhizal abundance but captured fertilization effects in a mesocosm experiment

Ven, Arne LU ; Verbruggen, Erik ; Verlinden, Melanie S. ; Olsson, Pål Axel LU ; Wallander, Håkan LU and Vicca, Sara (2020) In Plant and Soil 446(1-2). p.563-575
Abstract

Aims: Ingrowth bags are widely used to estimate mycorrhizal growth and dynamics. However, it remains unclear to what extent they reflect the surrounding soil, and how this varies with environmental conditions. Methods: We used a fertilization experiment to investigate if carbon-free mesh bags were representative of their surrounding soil. We determined AMF hyphal length density (HLD), phospholipid fatty acids (PLFA 16:1ω5) and neutral lipid fatty acids (NLFA 16:1ω5). Results: When AMF abundance in surrounding soil was high, HLD and both fatty acids were underestimated by the mesh bags. The PLFA 16:1ω5 in surrounding soil included bacterial PLFA, complicating the comparison of PLFA 16:1ω5 between mesh bags and surrounding soil. Both NLFA... (More)

Aims: Ingrowth bags are widely used to estimate mycorrhizal growth and dynamics. However, it remains unclear to what extent they reflect the surrounding soil, and how this varies with environmental conditions. Methods: We used a fertilization experiment to investigate if carbon-free mesh bags were representative of their surrounding soil. We determined AMF hyphal length density (HLD), phospholipid fatty acids (PLFA 16:1ω5) and neutral lipid fatty acids (NLFA 16:1ω5). Results: When AMF abundance in surrounding soil was high, HLD and both fatty acids were underestimated by the mesh bags. The PLFA 16:1ω5 in surrounding soil included bacterial PLFA, complicating the comparison of PLFA 16:1ω5 between mesh bags and surrounding soil. Both NLFA 16:1ω5 and HLD showed a significantly positive correlation, and fertilization effects were mostly similar for mesh bags and surrounding soil. Conclusions: Although carbon-free mesh bags can underestimate AMF abundance in soils, they represent a useful method to compare patterns in AMF abundance across environmental gradients and can be particularly useful in combination with the use of stable isotope tracers for unraveling patterns in AMF growth. NLFA 16:1ω5 appeared a more accurate measure for AMF than PLFA 16:1ω5 because the latter included bacterial PLFA.

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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Hyphal length density (HLD), Mycorrhizal fungi, Neutral lipid fatty acid (NLFA), Phospholipid fatty acid (PLFA), Stable carbon isotopes
in
Plant and Soil
volume
446
issue
1-2
pages
13 pages
publisher
Springer
external identifiers
  • scopus:85075901258
ISSN
0032-079X
DOI
10.1007/s11104-019-04368-4
language
English
LU publication?
yes
id
baa136bb-dc93-4fff-ab35-dcd64cbfbca5
date added to LUP
2021-01-04 11:00:15
date last changed
2021-01-19 04:01:31
@article{baa136bb-dc93-4fff-ab35-dcd64cbfbca5,
  abstract     = {<p>Aims: Ingrowth bags are widely used to estimate mycorrhizal growth and dynamics. However, it remains unclear to what extent they reflect the surrounding soil, and how this varies with environmental conditions. Methods: We used a fertilization experiment to investigate if carbon-free mesh bags were representative of their surrounding soil. We determined AMF hyphal length density (HLD), phospholipid fatty acids (PLFA 16:1ω5) and neutral lipid fatty acids (NLFA 16:1ω5). Results: When AMF abundance in surrounding soil was high, HLD and both fatty acids were underestimated by the mesh bags. The PLFA 16:1ω5 in surrounding soil included bacterial PLFA, complicating the comparison of PLFA 16:1ω5 between mesh bags and surrounding soil. Both NLFA 16:1ω5 and HLD showed a significantly positive correlation, and fertilization effects were mostly similar for mesh bags and surrounding soil. Conclusions: Although carbon-free mesh bags can underestimate AMF abundance in soils, they represent a useful method to compare patterns in AMF abundance across environmental gradients and can be particularly useful in combination with the use of stable isotope tracers for unraveling patterns in AMF growth. NLFA 16:1ω5 appeared a more accurate measure for AMF than PLFA 16:1ω5 because the latter included bacterial PLFA.</p>},
  author       = {Ven, Arne and Verbruggen, Erik and Verlinden, Melanie S. and Olsson, Pål Axel and Wallander, Håkan and Vicca, Sara},
  issn         = {0032-079X},
  language     = {eng},
  number       = {1-2},
  pages        = {563--575},
  publisher    = {Springer},
  series       = {Plant and Soil},
  title        = {Mesh bags underestimated arbuscular mycorrhizal abundance but captured fertilization effects in a mesocosm experiment},
  url          = {http://dx.doi.org/10.1007/s11104-019-04368-4},
  doi          = {10.1007/s11104-019-04368-4},
  volume       = {446},
  year         = {2020},
}