Macrophages from high-risk HLA-DQB1*0201/*0302 type 1 diabetes mellitus patients are hypersensitive to lipopolysaccharide stimulation.
Plesner, A ; Greenbaum, C J ; Gaur, L K ; Ernst, R K and Lernmark, Åke LU
(2002)
In Scandinavian Journal of Immunology
56(5).
p.522-529
- Abstract
- Levels of nonantigen-induced pro-inflammatory cytokines and prostaglandin in macrophages isolated from human leucocyte antigen (HLA)-matched type 1 diabetes mellitus patients, first-degree relatives and healthy controls were determined. We hypothesize that monocytes isolated from patients are sensitized or preactivated and therefore, have an altered response to in vitro stimulus compared with control groups as measured by levels of pro- and anti-inflammatory mediators. In this study, peripheral blood monocytes were differentiated to macrophages with macrophage-colony stimulating factor (M-CSF) to determine lipopolysaccharide (LPS)-stimulated tumour necrosis factor (TNF)-alpha, interleukin (IL)-1beta, IL-6, IL-12 and prostaglandin E-2... (More)
- Levels of nonantigen-induced pro-inflammatory cytokines and prostaglandin in macrophages isolated from human leucocyte antigen (HLA)-matched type 1 diabetes mellitus patients, first-degree relatives and healthy controls were determined. We hypothesize that monocytes isolated from patients are sensitized or preactivated and therefore, have an altered response to in vitro stimulus compared with control groups as measured by levels of pro- and anti-inflammatory mediators. In this study, peripheral blood monocytes were differentiated to macrophages with macrophage-colony stimulating factor (M-CSF) to determine lipopolysaccharide (LPS)-stimulated tumour necrosis factor (TNF)-alpha, interleukin (IL)-1beta, IL-6, IL-12 and prostaglandin E-2 (PGE-2) secretion from hetero- or homozygous HLA DQB1*0201 and *0302 type 1 diabetes mellitus patients, first-degree relatives and homozygous HLA DQB1*0602 healthy controls. LPS-stimulated secretion of TNF-alpha, IL-1beta and IL-6 was immediate and markedl (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1124791
- author
- Plesner, A
; Greenbaum, C J
; Gaur, L K
; Ernst, R K
and Lernmark, Åke
LU
- publishing date
- 2002
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Scandinavian Journal of Immunology
- volume
- 56
- issue
- 5
- pages
- 522 - 529
- publisher
- Wiley-Blackwell
- external identifiers
-
- scopus:0036436681
- ISSN
- 1365-3083
- DOI
- 10.1046/j.1365-3083.2002.01150.x
- language
- English
- LU publication?
- no
- id
- bab502d2-1c3e-4030-adfd-60c35313c052 (old id 1124791)
- date added to LUP
- 2016-04-01 15:55:34
- date last changed
- 2022-02-05 03:59:06
@article{bab502d2-1c3e-4030-adfd-60c35313c052,
abstract = {{Levels of nonantigen-induced pro-inflammatory cytokines and prostaglandin in macrophages isolated from human leucocyte antigen (HLA)-matched type 1 diabetes mellitus patients, first-degree relatives and healthy controls were determined. We hypothesize that monocytes isolated from patients are sensitized or preactivated and therefore, have an altered response to in vitro stimulus compared with control groups as measured by levels of pro- and anti-inflammatory mediators. In this study, peripheral blood monocytes were differentiated to macrophages with macrophage-colony stimulating factor (M-CSF) to determine lipopolysaccharide (LPS)-stimulated tumour necrosis factor (TNF)-alpha, interleukin (IL)-1beta, IL-6, IL-12 and prostaglandin E-2 (PGE-2) secretion from hetero- or homozygous HLA DQB1*0201 and *0302 type 1 diabetes mellitus patients, first-degree relatives and homozygous HLA DQB1*0602 healthy controls. LPS-stimulated secretion of TNF-alpha, IL-1beta and IL-6 was immediate and markedl}},
author = {{Plesner, A and Greenbaum, C J and Gaur, L K and Ernst, R K and Lernmark, Åke}},
issn = {{1365-3083}},
language = {{eng}},
number = {{5}},
pages = {{522--529}},
publisher = {{Wiley-Blackwell}},
series = {{Scandinavian Journal of Immunology}},
title = {{Macrophages from high-risk HLA-DQB1*0201/*0302 type 1 diabetes mellitus patients are hypersensitive to lipopolysaccharide stimulation.}},
url = {{http://dx.doi.org/10.1046/j.1365-3083.2002.01150.x}},
doi = {{10.1046/j.1365-3083.2002.01150.x}},
volume = {{56}},
year = {{2002}},
}