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Generation of a conditionally null allele of the laminin alpha 1 gene

Alpy, F ; Jivkov, I ; Sorokin, Lydia LU ; Klein, A ; Arnold, C ; Huss, Y ; Kedinger, M ; Simon-Assmann, R and Lefebvre, O (2005) In Genesis: The Journal of Genetics and Development 43(2). p.59-70
Abstract
Laminins are heterotrimeric glycoproteins of the basement membranes. Laminin 1 (alpha 1, beta 1, gamma 1) is the major laminin expressed during early mouse embryogenesis. To gain access, to the physiological function of laminin alpha 1 chain, we developed a conditionally null allele of its encoding gene (Lama1) using the cre/loxP system. Floxed-allele-carrying mice (Lama1(flox/flox)) display no overt phenotype. Lama1(flox/flox) mice were crossed with transgenic deleter mice (CMV-Cre) to generate Lama1-deficient mice (Lama1(Delta/Delta)). Lama1(Delta/Delta) embryos die during the early postimplantation period after embryonic day 6.5. They lack Reichert's membrane, an extraembryonic basement membrane in which laminin all is normally highly... (More)
Laminins are heterotrimeric glycoproteins of the basement membranes. Laminin 1 (alpha 1, beta 1, gamma 1) is the major laminin expressed during early mouse embryogenesis. To gain access, to the physiological function of laminin alpha 1 chain, we developed a conditionally null allele of its encoding gene (Lama1) using the cre/loxP system. Floxed-allele-carrying mice (Lama1(flox/flox)) display no overt phenotype. Lama1(flox/flox) mice were crossed with transgenic deleter mice (CMV-Cre) to generate Lama1-deficient mice (Lama1(Delta/Delta)). Lama1(Delta/Delta) embryos die during the early postimplantation period after embryonic day 6.5. They lack Reichert's membrane, an extraembryonic basement membrane in which laminin all is normally highly expressed. In parallel, Lama1(Delta/Delta) embryos display 1) parietal and visceral endoderm differentiation defects with altered expression of cytokeratin 19 and GATA4, respectively, and 2) an induction of apoptosis. This new mouse model is of particular interest as it will allow time- and tissue-specific inactivation of the Lamal gene in various organs. genesis 43:59-70, 2005. (Less)
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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Reichert's membrane, development, apoptosis, GATA4, conditional inactivation, Lama1 genes, knockout, basement membrane
in
Genesis: The Journal of Genetics and Development
volume
43
issue
2
pages
59 - 70
publisher
John Wiley and Sons
external identifiers
  • wos:000233060100002
  • pmid:16100707
  • scopus:27744522715
ISSN
1526-954X
DOI
10.1002/gene.20154
language
English
LU publication?
yes
additional info
The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Pathology, (Lund) (013030000)
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bac70cbc-4854-4b7d-ba81-91452a948b42 (old id 214239)
date added to LUP
2016-04-01 12:27:45
date last changed
2021-01-06 01:42:56
@article{bac70cbc-4854-4b7d-ba81-91452a948b42,
  abstract     = {Laminins are heterotrimeric glycoproteins of the basement membranes. Laminin 1 (alpha 1, beta 1, gamma 1) is the major laminin expressed during early mouse embryogenesis. To gain access, to the physiological function of laminin alpha 1 chain, we developed a conditionally null allele of its encoding gene (Lama1) using the cre/loxP system. Floxed-allele-carrying mice (Lama1(flox/flox)) display no overt phenotype. Lama1(flox/flox) mice were crossed with transgenic deleter mice (CMV-Cre) to generate Lama1-deficient mice (Lama1(Delta/Delta)). Lama1(Delta/Delta) embryos die during the early postimplantation period after embryonic day 6.5. They lack Reichert's membrane, an extraembryonic basement membrane in which laminin all is normally highly expressed. In parallel, Lama1(Delta/Delta) embryos display 1) parietal and visceral endoderm differentiation defects with altered expression of cytokeratin 19 and GATA4, respectively, and 2) an induction of apoptosis. This new mouse model is of particular interest as it will allow time- and tissue-specific inactivation of the Lamal gene in various organs. genesis 43:59-70, 2005.},
  author       = {Alpy, F and Jivkov, I and Sorokin, Lydia and Klein, A and Arnold, C and Huss, Y and Kedinger, M and Simon-Assmann, R and Lefebvre, O},
  issn         = {1526-954X},
  language     = {eng},
  number       = {2},
  pages        = {59--70},
  publisher    = {John Wiley and Sons},
  series       = {Genesis: The Journal of Genetics and Development},
  title        = {Generation of a conditionally null allele of the laminin alpha 1 gene},
  url          = {http://dx.doi.org/10.1002/gene.20154},
  doi          = {10.1002/gene.20154},
  volume       = {43},
  year         = {2005},
}