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The pro-inflammatory effects of particulate matter on epithelial cells are associated with elemental composition

Shao, Jingyi; Wheeler, Amanda J.; Chen, Ling; Strandberg, Bo LU ; Hinwood, Andrea; Johnston, Fay H. and Zosky, Graeme R. (2018) In Chemosphere 202. p.530-537
Abstract

Background: Adverse health effects of particulate matter (PM) vary with chemical composition; however, evidence regarding which elements are the most detrimental is limited. The roof space area provides an open and stable environment for outdoor PM to settle and deposit. Therefore, this study used roof space PM samples as a proxy of residential cumulative exposure to outdoor air pollution to investigate their pro-inflammatory effects on human lung cells and the contribution of the endotoxin and chemical content. Methods: Roof space PM samples of 36 different homes were collected and analysed using standardised techniques. We evaluated cytotoxicity and cytokine production of BEAS-2B cells after PM exposure using MTS and ELISA,... (More)

Background: Adverse health effects of particulate matter (PM) vary with chemical composition; however, evidence regarding which elements are the most detrimental is limited. The roof space area provides an open and stable environment for outdoor PM to settle and deposit. Therefore, this study used roof space PM samples as a proxy of residential cumulative exposure to outdoor air pollution to investigate their pro-inflammatory effects on human lung cells and the contribution of the endotoxin and chemical content. Methods: Roof space PM samples of 36 different homes were collected and analysed using standardised techniques. We evaluated cytotoxicity and cytokine production of BEAS-2B cells after PM exposure using MTS and ELISA, respectively. Principle component analysis (PCA) and linear regression analyses were employed to assess the associations between cytokine production and the PM components. Results: PM caused significant time- and dose-dependent increases in cellular cytokine production (p < 0.05). PCA identified four factors that explained 68.33% of the variance in the chemical composition. An increase in Factor 1 (+Fe, +Al, +Mn) score and a decrease in Factor 2 (-Ca, +Pb, +PAH) score were associated with increased interleukin (IL)-6 (Factor 1; p = 0.010; Factor 2; p = 0.006) and IL-8 (Factor 1; p = 0.003; Factor 2; p = 0.020) production, however, only the association with Factor 1 was evident after correcting for endotoxin and particle size. Conclusions: Our study provides novel insight into the positive associations between pro-inflammatory effects of roof space PM samples with Fe, Al and Mn levels.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Air pollution, Element, Inflammation, Particulate matter
in
Chemosphere
volume
202
pages
8 pages
publisher
Elsevier
external identifiers
  • scopus:85047487090
ISSN
0045-6535
DOI
10.1016/j.chemosphere.2018.03.052
language
English
LU publication?
yes
id
baebd4d8-25d0-439f-831a-ba5bfcef43cc
date added to LUP
2018-06-05 09:40:46
date last changed
2019-03-19 03:55:47
@article{baebd4d8-25d0-439f-831a-ba5bfcef43cc,
  abstract     = {<p>Background: Adverse health effects of particulate matter (PM) vary with chemical composition; however, evidence regarding which elements are the most detrimental is limited. The roof space area provides an open and stable environment for outdoor PM to settle and deposit. Therefore, this study used roof space PM samples as a proxy of residential cumulative exposure to outdoor air pollution to investigate their pro-inflammatory effects on human lung cells and the contribution of the endotoxin and chemical content. Methods: Roof space PM samples of 36 different homes were collected and analysed using standardised techniques. We evaluated cytotoxicity and cytokine production of BEAS-2B cells after PM exposure using MTS and ELISA, respectively. Principle component analysis (PCA) and linear regression analyses were employed to assess the associations between cytokine production and the PM components. Results: PM caused significant time- and dose-dependent increases in cellular cytokine production (p &lt; 0.05). PCA identified four factors that explained 68.33% of the variance in the chemical composition. An increase in Factor 1 (+Fe, +Al, +Mn) score and a decrease in Factor 2 (-Ca, +Pb, +PAH) score were associated with increased interleukin (IL)-6 (Factor 1; p = 0.010; Factor 2; p = 0.006) and IL-8 (Factor 1; p = 0.003; Factor 2; p = 0.020) production, however, only the association with Factor 1 was evident after correcting for endotoxin and particle size. Conclusions: Our study provides novel insight into the positive associations between pro-inflammatory effects of roof space PM samples with Fe, Al and Mn levels.</p>},
  author       = {Shao, Jingyi and Wheeler, Amanda J. and Chen, Ling and Strandberg, Bo and Hinwood, Andrea and Johnston, Fay H. and Zosky, Graeme R.},
  issn         = {0045-6535},
  keyword      = {Air pollution,Element,Inflammation,Particulate matter},
  language     = {eng},
  month        = {07},
  pages        = {530--537},
  publisher    = {Elsevier},
  series       = {Chemosphere},
  title        = {The pro-inflammatory effects of particulate matter on epithelial cells are associated with elemental composition},
  url          = {http://dx.doi.org/10.1016/j.chemosphere.2018.03.052},
  volume       = {202},
  year         = {2018},
}