Fluorescence imaging of light absorption for axial-beam geometry in capillary electrophoresis
(1998) In Electrophoresis 19(12). p.2233-2238- Abstract
- A new method for investigation of axial-beam absorption detection for improved detection limits in microcolumn separations is reported. The method is based on fluorescence imaging of light absorption along a separation capillary. The probing UV light is introduced at one end of the capillary and shows an exponential fall-off along the capillary. As the UV light propagates through the sample peaks, an additional loss in intensity will be observed. In order to view the absorption profile along the capillary, a background fluorophore is added to the buffer. A charge-coupled device (CCD) detector and imaging optics are placed beside the capillary to view the capillary in a direction perpendicular to the capillary. Signal integration is... (More)
- A new method for investigation of axial-beam absorption detection for improved detection limits in microcolumn separations is reported. The method is based on fluorescence imaging of light absorption along a separation capillary. The probing UV light is introduced at one end of the capillary and shows an exponential fall-off along the capillary. As the UV light propagates through the sample peaks, an additional loss in intensity will be observed. In order to view the absorption profile along the capillary, a background fluorophore is added to the buffer. A charge-coupled device (CCD) detector and imaging optics are placed beside the capillary to view the capillary in a direction perpendicular to the capillary. Signal integration is employed for consecutive exposures as well as for neighboring detector pixels in order to increase the signal-to-noise ratio. Measurements for stilbene 3 with sulforhodamine B as a background fluorophore are presented. The characteristics of the detection method and potential improvements are discussed. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/2258496
- author
- Johansson, Jonas LU ; Johansson, T and Nilsson, S
- organization
- publishing date
- 1998
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Electrophoresis
- volume
- 19
- issue
- 12
- pages
- 2233 - 2238
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- scopus:0031691149
- pmid:9761209
- ISSN
- 0173-0835
- DOI
- 10.1002/elps.1150191231
- language
- English
- LU publication?
- yes
- id
- bbbe329d-5b9c-4fc2-a487-6fdd9a0270f2 (old id 2258496)
- date added to LUP
- 2016-04-04 07:38:08
- date last changed
- 2022-01-29 02:22:05
@article{bbbe329d-5b9c-4fc2-a487-6fdd9a0270f2,
abstract = {{A new method for investigation of axial-beam absorption detection for improved detection limits in microcolumn separations is reported. The method is based on fluorescence imaging of light absorption along a separation capillary. The probing UV light is introduced at one end of the capillary and shows an exponential fall-off along the capillary. As the UV light propagates through the sample peaks, an additional loss in intensity will be observed. In order to view the absorption profile along the capillary, a background fluorophore is added to the buffer. A charge-coupled device (CCD) detector and imaging optics are placed beside the capillary to view the capillary in a direction perpendicular to the capillary. Signal integration is employed for consecutive exposures as well as for neighboring detector pixels in order to increase the signal-to-noise ratio. Measurements for stilbene 3 with sulforhodamine B as a background fluorophore are presented. The characteristics of the detection method and potential improvements are discussed.}},
author = {{Johansson, Jonas and Johansson, T and Nilsson, S}},
issn = {{0173-0835}},
language = {{eng}},
number = {{12}},
pages = {{2233--2238}},
publisher = {{John Wiley & Sons Inc.}},
series = {{Electrophoresis}},
title = {{Fluorescence imaging of light absorption for axial-beam geometry in capillary electrophoresis}},
url = {{https://lup.lub.lu.se/search/files/5146322/2297313.pdf}},
doi = {{10.1002/elps.1150191231}},
volume = {{19}},
year = {{1998}},
}