A simple and rapid microSepharose assay for GAD65 and ICA512 autoantibodies in diabetes
Schranz, Dorota B. ; Bekris, Lynn ; Landin-Olsson, Mona LU ; Törn, Carina LU ; Niläng, Anna ; Toll, Åsa ; Grönlund, Hans ; Toivola, Bert and Lernmark, Åke LU
(1998)
In Journal of Immunological Methods
213(1).
p.87-97
- Abstract
GAD65Ab and ICA512Ab are strongly associated with insulin-dependent (Type 1) diabetes mellitus. A novel, simple radio-antigen binding assay with microSepharose conjugated with monoclonal antibodies specific for human immunoglobulin light chains was developed to provide diagnostic sensitivity and specificity of GAD65Ab and ICA512Ab for Type 1 diabetes. The Receiver Operating Characteristic (ROC) curve was used to determine the upper level of Normal in 583 new onset Type 1 diabetic patients and in 829 matched controls. The sensitivity of GAD65Ab and ICA512Ab was 66% (384/583) and 41% (211/520), respectively, and the diagnostic specificity was 96% for both autoantibodies. Levels, but not frequency, of GAD65Ab were higher among female Type... (More)
GAD65Ab and ICA512Ab are strongly associated with insulin-dependent (Type 1) diabetes mellitus. A novel, simple radio-antigen binding assay with microSepharose conjugated with monoclonal antibodies specific for human immunoglobulin light chains was developed to provide diagnostic sensitivity and specificity of GAD65Ab and ICA512Ab for Type 1 diabetes. The Receiver Operating Characteristic (ROC) curve was used to determine the upper level of Normal in 583 new onset Type 1 diabetic patients and in 829 matched controls. The sensitivity of GAD65Ab and ICA512Ab was 66% (384/583) and 41% (211/520), respectively, and the diagnostic specificity was 96% for both autoantibodies. Levels, but not frequency, of GAD65Ab were higher among female Type 1 diabetes patients, whereas ICA512Ab levels did not differ between males and females. Positivity for GAD65Ab, ICA512Ab or both showed a sensitivity of 74% and a specificity of 92% for Type 1 diabetes. This simple, one-step centrifugation, high-capacity radio-antigen binding assay has a high precision and reproducibility to accurately detect both GAD65Ab and ICA512Ab. This assay should also prove useful in other autoantibody assays against conformation-sensitive autoantigens.
(Less)
- author
- Schranz, Dorota B.
; Bekris, Lynn
; Landin-Olsson, Mona
LU
; Törn, Carina
LU
; Niläng, Anna
; Toll, Åsa
; Grönlund, Hans
; Toivola, Bert
and Lernmark, Åke
LU
- publishing date
- 1998-04-01
- type
- Contribution to journal
- publication status
- published
- keywords
- Autoimmunity, Islet cell autoantibodies, MicroSepharose, Radio-antigen binding assay, ROC curve, Type 1 diabetes
- in
- Journal of Immunological Methods
- volume
- 213
- issue
- 1
- pages
- 87 - 97
- publisher
- Elsevier
- external identifiers
-
- scopus:0031818859
- pmid:9671127
- ISSN
- 0022-1759
- DOI
- 10.1016/S0022-1759(98)00025-8
- language
- English
- LU publication?
- no
- id
- bbe4a935-9dc6-4037-850f-b6a75c0813d7
- date added to LUP
- 2019-06-30 23:46:13
- date last changed
- 2024-03-13 08:01:23
@article{bbe4a935-9dc6-4037-850f-b6a75c0813d7,
abstract = {{<p>GAD65Ab and ICA512Ab are strongly associated with insulin-dependent (Type 1) diabetes mellitus. A novel, simple radio-antigen binding assay with microSepharose conjugated with monoclonal antibodies specific for human immunoglobulin light chains was developed to provide diagnostic sensitivity and specificity of GAD65Ab and ICA512Ab for Type 1 diabetes. The Receiver Operating Characteristic (ROC) curve was used to determine the upper level of Normal in 583 new onset Type 1 diabetic patients and in 829 matched controls. The sensitivity of GAD65Ab and ICA512Ab was 66% (384/583) and 41% (211/520), respectively, and the diagnostic specificity was 96% for both autoantibodies. Levels, but not frequency, of GAD65Ab were higher among female Type 1 diabetes patients, whereas ICA512Ab levels did not differ between males and females. Positivity for GAD65Ab, ICA512Ab or both showed a sensitivity of 74% and a specificity of 92% for Type 1 diabetes. This simple, one-step centrifugation, high-capacity radio-antigen binding assay has a high precision and reproducibility to accurately detect both GAD65Ab and ICA512Ab. This assay should also prove useful in other autoantibody assays against conformation-sensitive autoantigens.</p>}},
author = {{Schranz, Dorota B. and Bekris, Lynn and Landin-Olsson, Mona and Törn, Carina and Niläng, Anna and Toll, Åsa and Grönlund, Hans and Toivola, Bert and Lernmark, Åke}},
issn = {{0022-1759}},
keywords = {{Autoimmunity; Islet cell autoantibodies; MicroSepharose; Radio-antigen binding assay; ROC curve; Type 1 diabetes}},
language = {{eng}},
month = {{04}},
number = {{1}},
pages = {{87--97}},
publisher = {{Elsevier}},
series = {{Journal of Immunological Methods}},
title = {{A simple and rapid microSepharose assay for GAD65 and ICA512 autoantibodies in diabetes}},
url = {{http://dx.doi.org/10.1016/S0022-1759(98)00025-8}},
doi = {{10.1016/S0022-1759(98)00025-8}},
volume = {{213}},
year = {{1998}},
}