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Synthesis of alpha 1-microglobulin in cultured rat hepatocytes is stimulated by interleukin-6, leukemia inhibitory factor, dexamethasone and retinoic acid

Pierzchalski, P ; Rokita, H ; Koj, A ; Fries, E and Akerström, B LU (1992) In FEBS Letters 298(2-3). p.8-165
Abstract

The secretion of alpha 1-microglobulin by primary cultures of rat hepatocytes was found to increase upon the addition of interleukin-6 or leukemia inhibitory factor, two mediators of acute phase response. This stimulatory effect was further enhanced by dexamethasone. alpha 1-Microglobulin is synthesized as a precursor also containing bikunin, and the precursor protein is cleaved shortly before secretion. Our results therefore suggest that both alpha 1-microglobulin and bikunin are acute phase reactants in rat hepatocytes. Furthermore, we found that retinoic acid, previously shown to be involved in the regulation of cell differentiation and development, also stimulated alpha 1-microglobulin synthesis. Only free, uncomplexed alpha... (More)

The secretion of alpha 1-microglobulin by primary cultures of rat hepatocytes was found to increase upon the addition of interleukin-6 or leukemia inhibitory factor, two mediators of acute phase response. This stimulatory effect was further enhanced by dexamethasone. alpha 1-Microglobulin is synthesized as a precursor also containing bikunin, and the precursor protein is cleaved shortly before secretion. Our results therefore suggest that both alpha 1-microglobulin and bikunin are acute phase reactants in rat hepatocytes. Furthermore, we found that retinoic acid, previously shown to be involved in the regulation of cell differentiation and development, also stimulated alpha 1-microglobulin synthesis. Only free, uncomplexed alpha 1-microglobulin (28,000 Da) was detected in the hepatocyte media, suggesting that the complex between alpha 1-microglobulin and alpha 1-inhibitor 3, found in rat serum, is formed outside the hepatocyte.

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published
subject
keywords
Alpha-Globulins/biosynthesis, Animals, Cells, Cultured, Chromatography, Gel, Dexamethasone/pharmacology, Growth Inhibitors/pharmacology, Immunoblotting, Interleukin-6/pharmacology, Leukemia Inhibitory Factor, Liver/drug effects, Lymphokines/pharmacology, Rats, Rats, Inbred Strains, Tretinoin/pharmacology
in
FEBS Letters
volume
298
issue
2-3
pages
8 - 165
publisher
Wiley-Blackwell
external identifiers
  • pmid:1371972
  • scopus:0026570632
ISSN
0014-5793
DOI
10.1016/0014-5793(92)80047-K
language
English
LU publication?
yes
id
bc1a169d-ce62-4d62-9af7-95403084d146
date added to LUP
2019-05-22 10:25:21
date last changed
2021-01-03 06:29:46
@article{bc1a169d-ce62-4d62-9af7-95403084d146,
  abstract     = {<p>The secretion of alpha 1-microglobulin by primary cultures of rat hepatocytes was found to increase upon the addition of interleukin-6 or leukemia inhibitory factor, two mediators of acute phase response. This stimulatory effect was further enhanced by dexamethasone. alpha 1-Microglobulin is synthesized as a precursor also containing bikunin, and the precursor protein is cleaved shortly before secretion. Our results therefore suggest that both alpha 1-microglobulin and bikunin are acute phase reactants in rat hepatocytes. Furthermore, we found that retinoic acid, previously shown to be involved in the regulation of cell differentiation and development, also stimulated alpha 1-microglobulin synthesis. Only free, uncomplexed alpha 1-microglobulin (28,000 Da) was detected in the hepatocyte media, suggesting that the complex between alpha 1-microglobulin and alpha 1-inhibitor 3, found in rat serum, is formed outside the hepatocyte.</p>},
  author       = {Pierzchalski, P and Rokita, H and Koj, A and Fries, E and Akerström, B},
  issn         = {0014-5793},
  language     = {eng},
  month        = {02},
  number       = {2-3},
  pages        = {8--165},
  publisher    = {Wiley-Blackwell},
  series       = {FEBS Letters},
  title        = {Synthesis of alpha 1-microglobulin in cultured rat hepatocytes is stimulated by interleukin-6, leukemia inhibitory factor, dexamethasone and retinoic acid},
  url          = {http://dx.doi.org/10.1016/0014-5793(92)80047-K},
  doi          = {10.1016/0014-5793(92)80047-K},
  volume       = {298},
  year         = {1992},
}