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Adherent Intestinal Cells From Atlantic Salmon Show Phagocytic Ability and Express Macrophage-Specific Genes

Park, Youngjin ; Zhang, Qirui LU ; Wiegertjes, Geert F. ; Fernandes, Jorge M.O. and Kiron, Viswanath (2020) In Frontiers in Cell and Developmental Biology 8.
Abstract

Our knowledge of the intestinal immune system of fish is rather limited compared to mammals. Very little is known about the immune cells including the phagocytic cells in fish intestine. Hence, employing imaging flow cytometry and RNA sequencing, we studied adherent cells isolated from healthy Atlantic salmon. Phagocytic activity and selected gene expression of adherent cells from the distal intestine (adherent intestinal cells, or AIC) were compared with those from head kidney (adherent kidney cells, or AKC). Phagocytic activity of the two cell types was assessed based on the uptake of Escherichia coli BioParticlesTM. AIC showed phagocytic ability but the phagocytes were of different morphology compared to AKC.... (More)

Our knowledge of the intestinal immune system of fish is rather limited compared to mammals. Very little is known about the immune cells including the phagocytic cells in fish intestine. Hence, employing imaging flow cytometry and RNA sequencing, we studied adherent cells isolated from healthy Atlantic salmon. Phagocytic activity and selected gene expression of adherent cells from the distal intestine (adherent intestinal cells, or AIC) were compared with those from head kidney (adherent kidney cells, or AKC). Phagocytic activity of the two cell types was assessed based on the uptake of Escherichia coli BioParticlesTM. AIC showed phagocytic ability but the phagocytes were of different morphology compared to AKC. Transcriptomic analysis revealed that AIC expressed genes associated with macrophages, T cells, and endothelial cells. Heatmap analysis of selected genes indicated that the adherent cells from the two organs had apparently higher expression of macrophage-related genes. We believe that the adherent intestinal cells have phagocytic characteristics and high expression of genes commonly associated with macrophages. We envisage the possibilities for future studies on enriched populations of adherent intestinal cells.

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author
; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
adherent, Atlantic salmon, ImageStream, intestinal cells, macrophages, phagocytosis, RNA-Seq
in
Frontiers in Cell and Developmental Biology
volume
8
article number
580848
publisher
Frontiers Media S. A.
external identifiers
  • pmid:33178695
  • scopus:85094624205
ISSN
2296-634X
DOI
10.3389/fcell.2020.580848
language
English
LU publication?
yes
id
bc20234e-d8cd-419c-ba0c-d8fd53c22ded
date added to LUP
2020-11-16 14:26:36
date last changed
2024-05-29 23:30:59
@article{bc20234e-d8cd-419c-ba0c-d8fd53c22ded,
  abstract     = {{<p>Our knowledge of the intestinal immune system of fish is rather limited compared to mammals. Very little is known about the immune cells including the phagocytic cells in fish intestine. Hence, employing imaging flow cytometry and RNA sequencing, we studied adherent cells isolated from healthy Atlantic salmon. Phagocytic activity and selected gene expression of adherent cells from the distal intestine (adherent intestinal cells, or AIC) were compared with those from head kidney (adherent kidney cells, or AKC). Phagocytic activity of the two cell types was assessed based on the uptake of Escherichia coli BioParticles<sup>TM</sup>. AIC showed phagocytic ability but the phagocytes were of different morphology compared to AKC. Transcriptomic analysis revealed that AIC expressed genes associated with macrophages, T cells, and endothelial cells. Heatmap analysis of selected genes indicated that the adherent cells from the two organs had apparently higher expression of macrophage-related genes. We believe that the adherent intestinal cells have phagocytic characteristics and high expression of genes commonly associated with macrophages. We envisage the possibilities for future studies on enriched populations of adherent intestinal cells.</p>}},
  author       = {{Park, Youngjin and Zhang, Qirui and Wiegertjes, Geert F. and Fernandes, Jorge M.O. and Kiron, Viswanath}},
  issn         = {{2296-634X}},
  keywords     = {{adherent; Atlantic salmon; ImageStream; intestinal cells; macrophages; phagocytosis; RNA-Seq}},
  language     = {{eng}},
  month        = {{10}},
  publisher    = {{Frontiers Media S. A.}},
  series       = {{Frontiers in Cell and Developmental Biology}},
  title        = {{Adherent Intestinal Cells From Atlantic Salmon Show Phagocytic Ability and Express Macrophage-Specific Genes}},
  url          = {{http://dx.doi.org/10.3389/fcell.2020.580848}},
  doi          = {{10.3389/fcell.2020.580848}},
  volume       = {{8}},
  year         = {{2020}},
}