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Cisterna Magna Injection in Rats to Study Glymphatic Function

Ramos, Marta LU ; Burdon Bechet, Nicholas LU ; Battistella, Roberta LU ; Pavan, Chiara LU ; Xavier, Anna L.R.; Nedergaard, Maiken and Lundgaard, Iben LU (2019) In Methods in molecular biology (Clifton, N.J.) 1938. p.97-104
Abstract

The recently discovered glymphatic system, which supports brain-wide clearance of metabolic waste, has become the subject of intense research within the past few years. Its nomenclature arose due to its functionally analogous nature to the lymphatic system in combination with glial cells that are part of its anatomical boundaries. The influx of cerebrospinal fluid (CSF) from perivascular spaces into the brain interstitium acts to clear intraparenchymal solutes. CSF is produced by the choroid plexus and flows from the ventricles to the subarachnoid space via the cisterna magna, and as such the injection of tracer molecules into any one of these spaces could be used for studying CSF movement through the glymphatic system. Of these... (More)

The recently discovered glymphatic system, which supports brain-wide clearance of metabolic waste, has become the subject of intense research within the past few years. Its nomenclature arose due to its functionally analogous nature to the lymphatic system in combination with glial cells that are part of its anatomical boundaries. The influx of cerebrospinal fluid (CSF) from perivascular spaces into the brain interstitium acts to clear intraparenchymal solutes. CSF is produced by the choroid plexus and flows from the ventricles to the subarachnoid space via the cisterna magna, and as such the injection of tracer molecules into any one of these spaces could be used for studying CSF movement through the glymphatic system. Of these options, the cisterna magna is most favorable as it offers a route of entry that does not involve craniotomy. Herein we describe the cisterna magna (CM) injection procedure carried out in rats, essential for studying glymphatic influx and efflux dynamics.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Cerebrospinal fluid, Cisterna magna injection, Glymphatic system, Tracer
in
Methods in molecular biology (Clifton, N.J.)
volume
1938
pages
8 pages
publisher
Springer
external identifiers
  • scopus:85059928469
ISSN
1940-6029
DOI
10.1007/978-1-4939-9068-9_7
language
English
LU publication?
yes
id
bc2fb7f4-4f27-4196-8771-9ddeb8f616f2
date added to LUP
2019-01-24 12:22:08
date last changed
2019-02-20 11:44:25
@article{bc2fb7f4-4f27-4196-8771-9ddeb8f616f2,
  abstract     = {<p>The recently discovered glymphatic system, which supports brain-wide clearance of metabolic waste, has become the subject of intense research within the past few years. Its nomenclature arose due to its functionally analogous nature to the lymphatic system in combination with glial cells that are part of its anatomical boundaries. The influx of cerebrospinal fluid (CSF) from perivascular spaces into the brain interstitium acts to clear intraparenchymal solutes. CSF is produced by the choroid plexus and flows from the ventricles to the subarachnoid space via the cisterna magna, and as such the injection of tracer molecules into any one of these spaces could be used for studying CSF movement through the glymphatic system. Of these options, the cisterna magna is most favorable as it offers a route of entry that does not involve craniotomy. Herein we describe the cisterna magna (CM) injection procedure carried out in rats, essential for studying glymphatic influx and efflux dynamics.</p>},
  author       = {Ramos, Marta and Burdon Bechet, Nicholas and Battistella, Roberta and Pavan, Chiara and Xavier, Anna L.R. and Nedergaard, Maiken and Lundgaard, Iben},
  issn         = {1940-6029},
  keyword      = {Cerebrospinal fluid,Cisterna magna injection,Glymphatic system,Tracer},
  language     = {eng},
  pages        = {97--104},
  publisher    = {Springer},
  series       = {Methods in molecular biology (Clifton, N.J.)},
  title        = {Cisterna Magna Injection in Rats to Study Glymphatic Function},
  url          = {http://dx.doi.org/10.1007/978-1-4939-9068-9_7},
  volume       = {1938},
  year         = {2019},
}