GUTI: a new antigen in the Cromer blood group system
(2003) In Transfusion 43(3). p.340-344- Abstract
- BACKGROUND: The Cromer blood group system consists of seven high-incidence and three low-incidence antigens carried on decay-accelerating factor (DAF). This report describes the identification and characterization of a new Cromer high-incidence antigen, named GUTI. STUDY DESIGN AND METHODS: RT-PCR and sequence analysis were performed on cDNA prepared from a Chilean donor whose serum contained the alloantibody (anti-GUTI). Based on the observed point mutation, a PCR-RFLP assay using MaeII was developed. To map the epitope, DAF-deletion mutants were tested by immunoblotting with anti-GUTI. RESULTS: Sequence analysis revealed a substitution of 719G>A in DAF in the proband. The proband's parents and two daughters were heterozygotes for... (More)
- BACKGROUND: The Cromer blood group system consists of seven high-incidence and three low-incidence antigens carried on decay-accelerating factor (DAF). This report describes the identification and characterization of a new Cromer high-incidence antigen, named GUTI. STUDY DESIGN AND METHODS: RT-PCR and sequence analysis were performed on cDNA prepared from a Chilean donor whose serum contained the alloantibody (anti-GUTI). Based on the observed point mutation, a PCR-RFLP assay using MaeII was developed. To map the epitope, DAF-deletion mutants were tested by immunoblotting with anti-GUTI. RESULTS: Sequence analysis revealed a substitution of 719G>A in DAF in the proband. The proband's parents and two daughters were heterozygotes for 719G>A, one sister whose RBCs typed GUTI- was homozygous for 719A, and one sister had the wild-type DAF (719G). Seven additional heterozygote samples were identified among 214 Chileans. No heterozygotes were found in 197 New York donors. Analysis using DAF-deletion mutants showed the antigenic determinant to be within short consensus repeat (SCR) 4. CONCLUSION: This study describes a novel high- incidence antigen (GUTI) in the Cromer blood group system characterized by the amino acid arginine at position 206 in SCR4 of DAF. The GUTI-negative proband has a substitution mutation that predicts for histidine at this position. (Less)
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https://lup.lub.lu.se/record/1126512
- author
- publishing date
- 2003
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Transfusion
- volume
- 43
- issue
- 3
- pages
- 340 - 344
- publisher
- Wiley-Blackwell
- external identifiers
-
- pmid:12675719
- scopus:0037342364
- ISSN
- 1537-2995
- DOI
- 10.1046/j.1537-2995.2003.00319.x
- language
- English
- LU publication?
- no
- id
- bc4cff75-b87b-4caa-9af4-73460e753a4e (old id 1126512)
- date added to LUP
- 2016-04-01 17:06:01
- date last changed
- 2022-01-29 00:22:59
@article{bc4cff75-b87b-4caa-9af4-73460e753a4e, abstract = {{BACKGROUND: The Cromer blood group system consists of seven high-incidence and three low-incidence antigens carried on decay-accelerating factor (DAF). This report describes the identification and characterization of a new Cromer high-incidence antigen, named GUTI. STUDY DESIGN AND METHODS: RT-PCR and sequence analysis were performed on cDNA prepared from a Chilean donor whose serum contained the alloantibody (anti-GUTI). Based on the observed point mutation, a PCR-RFLP assay using MaeII was developed. To map the epitope, DAF-deletion mutants were tested by immunoblotting with anti-GUTI. RESULTS: Sequence analysis revealed a substitution of 719G>A in DAF in the proband. The proband's parents and two daughters were heterozygotes for 719G>A, one sister whose RBCs typed GUTI- was homozygous for 719A, and one sister had the wild-type DAF (719G). Seven additional heterozygote samples were identified among 214 Chileans. No heterozygotes were found in 197 New York donors. Analysis using DAF-deletion mutants showed the antigenic determinant to be within short consensus repeat (SCR) 4. CONCLUSION: This study describes a novel high- incidence antigen (GUTI) in the Cromer blood group system characterized by the amino acid arginine at position 206 in SCR4 of DAF. The GUTI-negative proband has a substitution mutation that predicts for histidine at this position.}}, author = {{Storry, Jill and Sausais, Laima and Hue-Roye, Kim and Mudiwa, Flora and Ferrer, Zennie and Blajchman, Morris A and Lublin, Douglas M and Ma, Bei-Wen and Miquel, Juan F and Nervi, Flavio and Pereira, Jaime and Reid, Marion E}}, issn = {{1537-2995}}, language = {{eng}}, number = {{3}}, pages = {{340--344}}, publisher = {{Wiley-Blackwell}}, series = {{Transfusion}}, title = {{GUTI: a new antigen in the Cromer blood group system}}, url = {{http://dx.doi.org/10.1046/j.1537-2995.2003.00319.x}}, doi = {{10.1046/j.1537-2995.2003.00319.x}}, volume = {{43}}, year = {{2003}}, }