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Effects of islet cell surface antibodies and complement on the release of insulin and chromium from perifused β cells

Kanatsuna, T. ; Freedman, Z. R. ; Rubenstein, A. H. and Lernmark, A. LU orcid (1982) In Clinical and Experimental Immunology 47(1). p.85-92
Abstract


The dynamics of insulin and chromium release from prelabelled rat pancreatic islet cells were studied by perifusion of cells supported in a column of Bio-Gel P-2 polyacrylamide beads. The column-perifused β cells released insulin in a biphasic pattern in response to 30 mmol/l D-glucose and in a monophasic pattern to 20 mmol/l L-arginine. Rat islet cells, first exposed to a rabbit anti-rat islet cell surface serum and complement and then added to the column, were unable to release insulin in response to 30 mmol/l D-glucose and 0.1 mmol/l 3-isobutyl-1-methylxanthine (IBMX). In order to study cytotoxicity by an additional approach, islet cells were prelabelled with radioactive chromium (Na
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The dynamics of insulin and chromium release from prelabelled rat pancreatic islet cells were studied by perifusion of cells supported in a column of Bio-Gel P-2 polyacrylamide beads. The column-perifused β cells released insulin in a biphasic pattern in response to 30 mmol/l D-glucose and in a monophasic pattern to 20 mmol/l L-arginine. Rat islet cells, first exposed to a rabbit anti-rat islet cell surface serum and complement and then added to the column, were unable to release insulin in response to 30 mmol/l D-glucose and 0.1 mmol/l 3-isobutyl-1-methylxanthine (IBMX). In order to study cytotoxicity by an additional approach, islet cells were prelabelled with radioactive chromium (Na
2
51
CrO
4
). These cells did not release either insulin or
51
Cr in response to glucose. Furthermore, exposure of the cells to surface antiserum and complement before perifusion did not induce either chromium or insulin release. Similar results were obtained when glucose alone or combined with surface antiserum was added to the perifusate bathing rat islet cells incubated with 0.1 mmol/l non-radioactive Na
2
CrO
4
before perifusion. However, a transient, dramatic release of insulin from these cells was induced by adding complement to the perifusion medium (containing surface antibodies). These results indicate that complement-dependent cytotoxicity of islet cell surface antibodes involves different phenomena. Firstly, the cytotoxic reaction results in a transient release of insulin whether the physiological release mechanisms were blocked by chromium or not. Secondly, in cells not treated with chromium the cytotoxic reactions renders the β cells unable to release insulin in response to glucose.

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author
; ; and
publishing date
type
Contribution to journal
publication status
published
subject
in
Clinical and Experimental Immunology
volume
47
issue
1
pages
8 pages
publisher
British Society for Immunology
external identifiers
  • pmid:7047026
  • scopus:0020072515
ISSN
0009-9104
language
English
LU publication?
no
id
bc5098de-7c47-4113-940c-6ed50f1e036b
date added to LUP
2019-09-16 15:28:52
date last changed
2024-03-13 08:27:30
@article{bc5098de-7c47-4113-940c-6ed50f1e036b,
  abstract     = {{<p><br>
                            The dynamics of insulin and chromium release from prelabelled rat pancreatic islet cells were studied by perifusion of cells supported in a column of Bio-Gel P-2 polyacrylamide beads. The column-perifused β cells released insulin in a biphasic pattern in response to 30 mmol/l D-glucose and in a monophasic pattern to 20 mmol/l L-arginine. Rat islet cells, first exposed to a rabbit anti-rat islet cell surface serum and complement and then added to the column, were unable to release insulin in response to 30 mmol/l D-glucose and 0.1 mmol/l 3-isobutyl-1-methylxanthine (IBMX). In order to study cytotoxicity by an additional approach, islet cells were prelabelled with radioactive chromium (Na<br>
                            <sub>2</sub><br>
                            <sup>51</sup><br>
                            CrO<br>
                            <sub>4</sub><br>
                            ). These cells did not release either insulin or <br>
                            <sup>51</sup><br>
                            Cr in response to glucose. Furthermore, exposure of the cells to surface antiserum and complement before perifusion did not induce either chromium or insulin release. Similar results were obtained when glucose alone or combined with surface antiserum was added to the perifusate bathing rat islet cells incubated with 0.1 mmol/l non-radioactive Na<br>
                            <sub>2</sub><br>
                            CrO<br>
                            <sub>4</sub><br>
                             before perifusion. However, a transient, dramatic release of insulin from these cells was induced by adding complement to the perifusion medium (containing surface antibodies). These results indicate that complement-dependent cytotoxicity of islet cell surface antibodes involves different phenomena. Firstly, the cytotoxic reaction results in a transient release of insulin whether the physiological release mechanisms were blocked by chromium or not. Secondly, in cells not treated with chromium the cytotoxic reactions renders the β cells unable to release insulin in response to glucose.<br>
                        </p>}},
  author       = {{Kanatsuna, T. and Freedman, Z. R. and Rubenstein, A. H. and Lernmark, A.}},
  issn         = {{0009-9104}},
  language     = {{eng}},
  month        = {{01}},
  number       = {{1}},
  pages        = {{85--92}},
  publisher    = {{British Society for Immunology}},
  series       = {{Clinical and Experimental Immunology}},
  title        = {{Effects of islet cell surface antibodies and complement on the release of insulin and chromium from perifused β cells}},
  volume       = {{47}},
  year         = {{1982}},
}