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Sources of diacylglycerols for phospholipid synthesis in rat liver

Sundler, R LU ; Akesson, B and Nilsson, A LU (1974) In Biochimica et biophysica acta 337(2). p.54-248
Abstract
The biosynthesis of molecular species of phosphatidylethanolamine and phosphatidylcholine in isolated rat liver parenchymal cells was studied with [32P]phosphate and [3H]glycerol as precursors. Addition to the cells of albumin-bound oleic or linoleic acid resulted in the synthesis of high proportions of dioleoyl- or dilinoleoyl-phospholipids, especially from labeled glycerol. The fact that these species contained a lower proportion of 32P than of 3H indicated that other diacylglycerols than those newly synthesized via phosphatidic acids took part in phospholipid synthesis. The composition of such diacylglycerols could be calculated and was found (a) to be essentially unaffected by the presence of oleic or linoleic acid during incubation,... (More)
The biosynthesis of molecular species of phosphatidylethanolamine and phosphatidylcholine in isolated rat liver parenchymal cells was studied with [32P]phosphate and [3H]glycerol as precursors. Addition to the cells of albumin-bound oleic or linoleic acid resulted in the synthesis of high proportions of dioleoyl- or dilinoleoyl-phospholipids, especially from labeled glycerol. The fact that these species contained a lower proportion of 32P than of 3H indicated that other diacylglycerols than those newly synthesized via phosphatidic acids took part in phospholipid synthesis. The composition of such diacylglycerols could be calculated and was found (a) to be essentially unaffected by the presence of oleic or linoleic acid during incubation, and (b) to be very similar to the mass composition of diacylglycerol units in the phosphatidyl-cholines. This suggests that they had been formed from the phosphatidylcholines, probably by the reversal of the cholinephosphotransferase (EC 2.7.8.2) reaction. These diacylglycerols contributed about 26 and 13 % of the total diacylglycerols incorporated into phosphatidylethanolamines and phosphatidylcholines, respectively. (Less)
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keywords
Animals, Choline, Chromatography, Thin Layer, Diglycerides/metabolism, Fatty Acids/analysis, Fatty Acids, Unsaturated/analysis, Glycerides/metabolism, Glycerol/metabolism, Linoleic Acids/pharmacology, Liver/cytology, Male, Oleic Acids/pharmacology, Phosphatidic Acids/metabolism, Phosphatidylcholines/biosynthesis, Phosphatidylethanolamines/biosynthesis, Phospholipids/biosynthesis, Phosphotransferases/metabolism, Rats, Tritium
in
Biochimica et biophysica acta
volume
337
issue
2
pages
7 pages
publisher
Elsevier
external identifiers
  • pmid:4433550
ISSN
0006-3002
DOI
10.1016/0005-2760(74)90206-9
language
English
LU publication?
yes
id
bc7f6df9-96ab-4844-9198-510eb94b0b2e
date added to LUP
2019-09-19 14:51:55
date last changed
2020-09-18 04:02:40
@article{bc7f6df9-96ab-4844-9198-510eb94b0b2e,
  abstract     = {The biosynthesis of molecular species of phosphatidylethanolamine and phosphatidylcholine in isolated rat liver parenchymal cells was studied with [32P]phosphate and [3H]glycerol as precursors. Addition to the cells of albumin-bound oleic or linoleic acid resulted in the synthesis of high proportions of dioleoyl- or dilinoleoyl-phospholipids, especially from labeled glycerol. The fact that these species contained a lower proportion of 32P than of 3H indicated that other diacylglycerols than those newly synthesized via phosphatidic acids took part in phospholipid synthesis. The composition of such diacylglycerols could be calculated and was found (a) to be essentially unaffected by the presence of oleic or linoleic acid during incubation, and (b) to be very similar to the mass composition of diacylglycerol units in the phosphatidyl-cholines. This suggests that they had been formed from the phosphatidylcholines, probably by the reversal of the cholinephosphotransferase (EC 2.7.8.2) reaction. These diacylglycerols contributed about 26 and 13 % of the total diacylglycerols incorporated into phosphatidylethanolamines and phosphatidylcholines, respectively.},
  author       = {Sundler, R and Akesson, B and Nilsson, A},
  issn         = {0006-3002},
  language     = {eng},
  month        = {02},
  number       = {2},
  pages        = {54--248},
  publisher    = {Elsevier},
  series       = {Biochimica et biophysica acta},
  title        = {Sources of diacylglycerols for phospholipid synthesis in rat liver},
  url          = {http://dx.doi.org/10.1016/0005-2760(74)90206-9},
  doi          = {10.1016/0005-2760(74)90206-9},
  volume       = {337},
  year         = {1974},
}