Energy transfer in multi-funnel systems quantitatively assessed by two-dimensional polarization imaging and single funnel approximation : From single molecules to ensembles
Shi, Juanzi LU ; Camacho, Rafael and Scheblykin, Ivan G. LU
(2022)
In Journal of Chemical Physics
156(7).
- Abstract
Two-dimensional polarization imaging (2D POLIM) is an experimental method where correlations between fluorescence excitation- and fluorescence emission-polarization properties are measured. One way to analyze 2D POLIM data is to apply a so-called single funnel approximation (SFA). The SFA allows for quantitative assessment of energy transfer between chromophores with identical spectra [homo-FRET (Förster resonance energy transfer)]. In this paper, we run a series of computer experiments to investigate the applicability of the analysis based on the SFA to various systems ranging from single multichromophoric systems to isotropic ensembles. By setting various scenarios of energy transfer between individual chromophores within a single... (More)
Two-dimensional polarization imaging (2D POLIM) is an experimental method where correlations between fluorescence excitation- and fluorescence emission-polarization properties are measured. One way to analyze 2D POLIM data is to apply a so-called single funnel approximation (SFA). The SFA allows for quantitative assessment of energy transfer between chromophores with identical spectra [homo-FRET (Förster resonance energy transfer)]. In this paper, we run a series of computer experiments to investigate the applicability of the analysis based on the SFA to various systems ranging from single multichromophoric systems to isotropic ensembles. By setting various scenarios of energy transfer between individual chromophores within a single object, we were able to define the borders of the practical application of SFA. It allowed us to reach a more comprehensive interpretation of the experimental data in terms of uncovering the internal arrangement of chromophores in the system and energy transfer between them. We also found that the SFA can always formally explain the data for isotropic ensembles and derived a formula connecting the energy funneling efficiency parameter and traditional fluorescence anisotropy.
(Less)
- author
- Shi, Juanzi
LU
; Camacho, Rafael
and Scheblykin, Ivan G.
LU
- organization
- publishing date
- 2022-02-21
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Chemical Physics
- volume
- 156
- issue
- 7
- article number
- 074108
- publisher
- American Institute of Physics (AIP)
- external identifiers
-
- pmid:35183085
- scopus:85125006292
- ISSN
- 0021-9606
- DOI
- 10.1063/5.0075005
- language
- English
- LU publication?
- yes
- id
- bc9373bb-6a9a-41db-b3e9-48d7547c80ce
- date added to LUP
- 2022-06-15 13:06:11
- date last changed
- 2024-09-05 21:38:17
@article{bc9373bb-6a9a-41db-b3e9-48d7547c80ce,
abstract = {{<p>Two-dimensional polarization imaging (2D POLIM) is an experimental method where correlations between fluorescence excitation- and fluorescence emission-polarization properties are measured. One way to analyze 2D POLIM data is to apply a so-called single funnel approximation (SFA). The SFA allows for quantitative assessment of energy transfer between chromophores with identical spectra [homo-FRET (Förster resonance energy transfer)]. In this paper, we run a series of computer experiments to investigate the applicability of the analysis based on the SFA to various systems ranging from single multichromophoric systems to isotropic ensembles. By setting various scenarios of energy transfer between individual chromophores within a single object, we were able to define the borders of the practical application of SFA. It allowed us to reach a more comprehensive interpretation of the experimental data in terms of uncovering the internal arrangement of chromophores in the system and energy transfer between them. We also found that the SFA can always formally explain the data for isotropic ensembles and derived a formula connecting the energy funneling efficiency parameter and traditional fluorescence anisotropy.</p>}},
author = {{Shi, Juanzi and Camacho, Rafael and Scheblykin, Ivan G.}},
issn = {{0021-9606}},
language = {{eng}},
month = {{02}},
number = {{7}},
publisher = {{American Institute of Physics (AIP)}},
series = {{Journal of Chemical Physics}},
title = {{Energy transfer in multi-funnel systems quantitatively assessed by two-dimensional polarization imaging and single funnel approximation : From single molecules to ensembles}},
url = {{http://dx.doi.org/10.1063/5.0075005}},
doi = {{10.1063/5.0075005}},
volume = {{156}},
year = {{2022}},
}