Development of an amperometric biosensor based on acetylcholine esterase covalently bound to a new support material
(1998) In Talanta 45(3). p.557-563- Abstract
- A new type of amperometric biosensor based on immobilised acetylcholine esterase was designed and constructed. The enzyme was immobilised on a flow-through working electrode, which was prepared from reticulated vitreous carbon (RVC) or from a composite material consisting of RVC and superporous agarose. The sensor was operated in FIA mode using acetylthiocholine as a substrate. The sensor responded to inhibitors such as paraoxon—10−9 mol was detected by the sensor in a non-optimised configuration. The practical lifetime of the sensor was at least 1 month.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1261779
- author
- Khayyami, M ; Pérez Pita, M T ; Peña Garcia, N ; Johansson, Gillis LU ; Danielsson, Bengt LU and Larsson, Per-Olof LU
- organization
- publishing date
- 1998
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Acetylthiocholine, Acetylcholine esterase, Biosensor, RVC-electrode, Amperometric, Thiocholine, Mediator, Superporous agarose gel
- in
- Talanta
- volume
- 45
- issue
- 3
- pages
- 557 - 563
- publisher
- Elsevier
- external identifiers
-
- pmid:18967037
- scopus:0031941469
- ISSN
- 1873-3573
- DOI
- 10.1016/S0039-9140(97)00182-3
- language
- English
- LU publication?
- yes
- id
- bccb2ac7-6838-4dfb-927c-c570fb7a254e (old id 1261779)
- date added to LUP
- 2016-04-01 16:39:23
- date last changed
- 2024-06-11 08:43:05
@article{bccb2ac7-6838-4dfb-927c-c570fb7a254e, abstract = {{A new type of amperometric biosensor based on immobilised acetylcholine esterase was designed and constructed. The enzyme was immobilised on a flow-through working electrode, which was prepared from reticulated vitreous carbon (RVC) or from a composite material consisting of RVC and superporous agarose. The sensor was operated in FIA mode using acetylthiocholine as a substrate. The sensor responded to inhibitors such as paraoxon—10−9 mol was detected by the sensor in a non-optimised configuration. The practical lifetime of the sensor was at least 1 month.}}, author = {{Khayyami, M and Pérez Pita, M T and Peña Garcia, N and Johansson, Gillis and Danielsson, Bengt and Larsson, Per-Olof}}, issn = {{1873-3573}}, keywords = {{Acetylthiocholine; Acetylcholine esterase; Biosensor; RVC-electrode; Amperometric; Thiocholine; Mediator; Superporous agarose gel}}, language = {{eng}}, number = {{3}}, pages = {{557--563}}, publisher = {{Elsevier}}, series = {{Talanta}}, title = {{Development of an amperometric biosensor based on acetylcholine esterase covalently bound to a new support material}}, url = {{http://dx.doi.org/10.1016/S0039-9140(97)00182-3}}, doi = {{10.1016/S0039-9140(97)00182-3}}, volume = {{45}}, year = {{1998}}, }