Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Protocol to model tumor hypoxia in vitro using real-time phosphorescence-based sensing of O2 gradients generated by metastatic cancer cells

Hosny, Noreen ; Shen, Kimberly ; Zhao, Yihua ; Qu, Junle ; Sun, Yusha ; Butler, George ; Amend, Sarah ; Hammarlund, Emma U. LU orcid ; Gatenby, Robert and Brown, Joel , et al. (2025) In STAR Protocols 6(4).
Abstract

Tumor hypoxia plays a critical role in cancer progression and therapeutic resistance. Here, we present a protocol for the self-generation of hypoxia by metastatic cancer cells using phosphorescence-based O2 sensing. We describe steps for phosphorescent film calibration, system assembly, and real-time imaging of hypoxia development. We then detail approaches to spatially map resulting O2 gradients. This protocol supports applications for studying key behaviors linked to metastatic progression, including motility and aerotaxis, under physiologically relevant hypoxic conditions. For complete details on the use and execution of this protocol, please refer to Hosny et al.

Please use this url to cite or link to this publication:
author
; ; ; ; ; ; ; ; and , et al. (More)
; ; ; ; ; ; ; ; ; ; ; ; ; and (Less)
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
STAR Protocols
volume
6
issue
4
article number
104163
publisher
Cell Press
external identifiers
  • pmid:41150854
  • scopus:105020987257
ISSN
2666-1667
DOI
10.1016/j.xpro.2025.104163
language
English
LU publication?
yes
id
bd05af7e-b4cb-4080-814b-b418736e7295
date added to LUP
2025-12-08 14:16:32
date last changed
2025-12-09 03:00:06
@article{bd05af7e-b4cb-4080-814b-b418736e7295,
  abstract     = {{<p>Tumor hypoxia plays a critical role in cancer progression and therapeutic resistance. Here, we present a protocol for the self-generation of hypoxia by metastatic cancer cells using phosphorescence-based O<sub>2</sub> sensing. We describe steps for phosphorescent film calibration, system assembly, and real-time imaging of hypoxia development. We then detail approaches to spatially map resulting O<sub>2</sub> gradients. This protocol supports applications for studying key behaviors linked to metastatic progression, including motility and aerotaxis, under physiologically relevant hypoxic conditions. For complete details on the use and execution of this protocol, please refer to Hosny et al.</p>}},
  author       = {{Hosny, Noreen and Shen, Kimberly and Zhao, Yihua and Qu, Junle and Sun, Yusha and Butler, George and Amend, Sarah and Hammarlund, Emma U. and Gatenby, Robert and Brown, Joel and Pienta, Kenneth J. and Phan, Trung V. and Boyer-Paulet, Stephano and Li, Shengkai and Austin, Robert H.}},
  issn         = {{2666-1667}},
  language     = {{eng}},
  number       = {{4}},
  publisher    = {{Cell Press}},
  series       = {{STAR Protocols}},
  title        = {{Protocol to model tumor hypoxia in vitro using real-time phosphorescence-based sensing of O<sub>2</sub> gradients generated by metastatic cancer cells}},
  url          = {{http://dx.doi.org/10.1016/j.xpro.2025.104163}},
  doi          = {{10.1016/j.xpro.2025.104163}},
  volume       = {{6}},
  year         = {{2025}},
}