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Flow Cytometry Assay of Plasmodium Falciparum-Specific B-Cell Proportions

Lugaajju, Allan LU ; Tijani, Muyideen Kolapo LU and Persson, Kristina Eva Maria LU (2022) In Methods in Molecular Biology 2470. p.681-688
Abstract

Detection of P. falciparum-specific subpopulations of B-cells is important for studies of immunity in malaria. This protocol relies on the photostability and protein loading capacity of carboxylated quantum dots to detect a broad range of different P. falciparum-specific B-cells. Infected red blood cell ghosts, obtained by permeabilization of infected cells with Streptolysin O, are coupled with carboxyl quantum dots using N-ethyl-N-dimethylaminopropyl-carbodiimide condensation. Immunophenotyping of P. falciparum-specific B-cells is performed by flow cytometry using Fc-receptor block, quantum dot-infected red blood cell ghost conjugates, and fluorochrome-conjugated anti-human CD19 mouse monoclonal antibodies.

Please use this url to cite or link to this publication:
author
; and
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
keywords
B-cells, Flow cytometry, Ghost infected red blood cells, Malaria, Plasmodium falciparum, Quantum dots
host publication
Methods in Molecular Biology
series title
Methods in Molecular Biology
volume
2470
pages
8 pages
publisher
Humana Press
external identifiers
  • pmid:35881383
  • scopus:85135370446
ISSN
1064-3745
1940-6029
ISBN
978-1-0716-2189-9
DOI
10.1007/978-1-0716-2189-9_51
language
English
LU publication?
yes
id
bd527981-23eb-420a-93f7-12174cd2e3df
date added to LUP
2022-09-12 11:03:28
date last changed
2025-03-07 21:28:07
@inbook{bd527981-23eb-420a-93f7-12174cd2e3df,
  abstract     = {{<p>Detection of P. falciparum-specific subpopulations of B-cells is important for studies of immunity in malaria. This protocol relies on the photostability and protein loading capacity of carboxylated quantum dots to detect a broad range of different P. falciparum-specific B-cells. Infected red blood cell ghosts, obtained by permeabilization of infected cells with Streptolysin O, are coupled with carboxyl quantum dots using N-ethyl-N-dimethylaminopropyl-carbodiimide condensation. Immunophenotyping of P. falciparum-specific B-cells is performed by flow cytometry using Fc-receptor block, quantum dot-infected red blood cell ghost conjugates, and fluorochrome-conjugated anti-human CD19 mouse monoclonal antibodies.</p>}},
  author       = {{Lugaajju, Allan and Tijani, Muyideen Kolapo and Persson, Kristina Eva Maria}},
  booktitle    = {{Methods in Molecular Biology}},
  isbn         = {{978-1-0716-2189-9}},
  issn         = {{1064-3745}},
  keywords     = {{B-cells; Flow cytometry; Ghost infected red blood cells; Malaria; Plasmodium falciparum; Quantum dots}},
  language     = {{eng}},
  pages        = {{681--688}},
  publisher    = {{Humana Press}},
  series       = {{Methods in Molecular Biology}},
  title        = {{Flow Cytometry Assay of Plasmodium Falciparum-Specific B-Cell Proportions}},
  url          = {{http://dx.doi.org/10.1007/978-1-0716-2189-9_51}},
  doi          = {{10.1007/978-1-0716-2189-9_51}},
  volume       = {{2470}},
  year         = {{2022}},
}