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Oxidation of methionine residues activates the high-threshold heat-sensitive ion channel TRPV2

Fricke, Tabea C. ; Echtermeyer, Frank ; Zielke, Johannes ; de la Roche, Jeanne ; Filipovic, Milos R. ; Claverol, Stéphane ; Herzog, Christine ; Tominaga, Makoto ; Pumroy, Ruth A. and Moiseenkova-Bell, Vera Y. , et al. (2019) In Proceedings of the National Academy of Sciences of the United States of America 116(48). p.24359-24365
Abstract

Thermosensitive transient receptor potential (TRP) ion channels detect changes in ambient temperature to regulate body temperature and temperature-dependent cellular activity. Rodent orthologs of TRP vanilloid 2 (TRPV2) are activated by nonphysiological heat exceeding 50 °C, and human TRPV2 is heat-insensitive. TRPV2 is required for phagocytic activity of macrophages which are rarely exposed to excessive heat, but what activates TRPV2 in vivo remains elusive. Here we describe the molecular mechanism of an oxidation-induced temperature-dependent gating of TRPV2. While high concentrations of H2O2 induce a modest sensitization of heat-induced inward currents, the oxidant chloramine-T (ChT), ultraviolet A light, and... (More)

Thermosensitive transient receptor potential (TRP) ion channels detect changes in ambient temperature to regulate body temperature and temperature-dependent cellular activity. Rodent orthologs of TRP vanilloid 2 (TRPV2) are activated by nonphysiological heat exceeding 50 °C, and human TRPV2 is heat-insensitive. TRPV2 is required for phagocytic activity of macrophages which are rarely exposed to excessive heat, but what activates TRPV2 in vivo remains elusive. Here we describe the molecular mechanism of an oxidation-induced temperature-dependent gating of TRPV2. While high concentrations of H2O2 induce a modest sensitization of heat-induced inward currents, the oxidant chloramine-T (ChT), ultraviolet A light, and photosensitizing agents producing reactive oxygen species (ROS) activate and sensitize TRPV2. This oxidation-induced activation also occurs in excised inside-out membrane patches, indicating a direct effect on TRPV2. The reducing agent dithiothreitol (DTT) in combination with methionine sulfoxide reductase partially reverses ChT-induced sensitization, and the substitution of the methionine (M) residues M528 and M607 to isoleucine almost abolishes oxidation-induced gating of rat TRPV2. Mass spectrometry on purified rat TRPV2 protein confirms oxidation of these residues. Finally, macrophages generate TRPV2-like heat-induced inward currents upon oxidation and exhibit reduced phagocytosis when exposed to the TRP channel inhibitor ruthenium red (RR) or to DTT. In summary, our data reveal a methionine-dependent redox sensitivity of TRPV2 which may be an important endogenous mechanism for regulation of TRPV2 activity and account for its pivotal role for phagocytosis in macrophages.

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publication status
published
subject
keywords
Methionine, Oxidation, Phagocytosis, Redox sensitivity, TRPV2
in
Proceedings of the National Academy of Sciences of the United States of America
volume
116
issue
48
pages
7 pages
publisher
National Acad Sciences
external identifiers
  • scopus:85075524504
  • pmid:31719194
ISSN
0027-8424
DOI
10.1073/pnas.1904332116
language
English
LU publication?
yes
id
bd62b3ec-8b36-40d3-b454-9d784888d93d
date added to LUP
2019-12-04 15:14:50
date last changed
2019-12-05 02:27:47
@article{bd62b3ec-8b36-40d3-b454-9d784888d93d,
  abstract     = {<p>Thermosensitive transient receptor potential (TRP) ion channels detect changes in ambient temperature to regulate body temperature and temperature-dependent cellular activity. Rodent orthologs of TRP vanilloid 2 (TRPV2) are activated by nonphysiological heat exceeding 50 °C, and human TRPV2 is heat-insensitive. TRPV2 is required for phagocytic activity of macrophages which are rarely exposed to excessive heat, but what activates TRPV2 in vivo remains elusive. Here we describe the molecular mechanism of an oxidation-induced temperature-dependent gating of TRPV2. While high concentrations of H<sub>2</sub>O<sub>2</sub> induce a modest sensitization of heat-induced inward currents, the oxidant chloramine-T (ChT), ultraviolet A light, and photosensitizing agents producing reactive oxygen species (ROS) activate and sensitize TRPV2. This oxidation-induced activation also occurs in excised inside-out membrane patches, indicating a direct effect on TRPV2. The reducing agent dithiothreitol (DTT) in combination with methionine sulfoxide reductase partially reverses ChT-induced sensitization, and the substitution of the methionine (M) residues M528 and M607 to isoleucine almost abolishes oxidation-induced gating of rat TRPV2. Mass spectrometry on purified rat TRPV2 protein confirms oxidation of these residues. Finally, macrophages generate TRPV2-like heat-induced inward currents upon oxidation and exhibit reduced phagocytosis when exposed to the TRP channel inhibitor ruthenium red (RR) or to DTT. In summary, our data reveal a methionine-dependent redox sensitivity of TRPV2 which may be an important endogenous mechanism for regulation of TRPV2 activity and account for its pivotal role for phagocytosis in macrophages.</p>},
  author       = {Fricke, Tabea C. and Echtermeyer, Frank and Zielke, Johannes and de la Roche, Jeanne and Filipovic, Milos R. and Claverol, Stéphane and Herzog, Christine and Tominaga, Makoto and Pumroy, Ruth A. and Moiseenkova-Bell, Vera Y. and Zygmunt, Peter M. and Leffler, Andreas and Eberhardt, Mirjam J.},
  issn         = {0027-8424},
  language     = {eng},
  number       = {48},
  pages        = {24359--24365},
  publisher    = {National Acad Sciences},
  series       = {Proceedings of the National Academy of Sciences of the United States of America},
  title        = {Oxidation of methionine residues activates the high-threshold heat-sensitive ion channel TRPV2},
  url          = {http://dx.doi.org/10.1073/pnas.1904332116},
  doi          = {10.1073/pnas.1904332116},
  volume       = {116},
  year         = {2019},
}