Immunoglobulin production induced by CD57+ GC-derived helper T cells in vitro requires addition of exogenous IL-2
(1996) In Cellular Immunology 169(2). p.166-173- Abstract
Germinal centers (GC) are well-defined areas in lymphoid organs were B cells proliferate and differentiate in response to T-cell-dependent antigens. The GC comprises B cells, follicular dendritic cells, tangible body macrophages, and a low number of CD4+ T cells. A large portion of these T cells expresses CD57. We have examined the ability of the CD4+CD57+ GC T cells to become activated and to take part in B cell activation processes, These T cells coexpress CD45RO, CD69, CD28, and upon mitogenic stimulation CD25, The cell population was found neither to containe nor to be able to produce any specific mRNA for IL-2, IL-4, and IFN-γ upon activation. Levels of mRNA encoding CD40 ligand was also... (More)
Germinal centers (GC) are well-defined areas in lymphoid organs were B cells proliferate and differentiate in response to T-cell-dependent antigens. The GC comprises B cells, follicular dendritic cells, tangible body macrophages, and a low number of CD4+ T cells. A large portion of these T cells expresses CD57. We have examined the ability of the CD4+CD57+ GC T cells to become activated and to take part in B cell activation processes, These T cells coexpress CD45RO, CD69, CD28, and upon mitogenic stimulation CD25, The cell population was found neither to containe nor to be able to produce any specific mRNA for IL-2, IL-4, and IFN-γ upon activation. Levels of mRNA encoding CD40 ligand was also undetectable under similar conditions. Furthermore, in contrast to ordinary CD4+ T cells, this population expressing CD57 was unable to induce B cells to Ig production in the presence of pokeweed mitogen or SEA unless IL-2 was added to the cultures, However, despite their apparent lack of function CD4+CD57+ GC T cells were found to rescue GC B cells from cell death in vitro to the same extent as CD4+CD57- T(h) cells, The phenotypical and functional differences found between these T cells and regular T(h)-cells suggest that they either represent a T cell subset with distinct properties within the GC yet to be determined or that they represent T cells, Irate in the immune response, having lost most of their original functions and capabilities.
(Less)
- author
- Andersson, Eva LU ; Dahlenborg, Katarina LU ; Ohlin, Mats LU ; Borrebaeck, Carl A K LU and Carlsson, Roland LU
- organization
- publishing date
- 1996-05-01
- type
- Contribution to journal
- publication status
- published
- in
- Cellular Immunology
- volume
- 169
- issue
- 2
- pages
- 8 pages
- publisher
- Elsevier
- external identifiers
-
- pmid:8620544
- scopus:0029943961
- ISSN
- 0008-8749
- DOI
- 10.1006/cimm.1996.0107
- language
- English
- LU publication?
- yes
- id
- bd692bd6-222d-48e1-aa6b-3a360ba2a97f
- date added to LUP
- 2016-04-19 14:08:35
- date last changed
- 2024-04-04 20:21:09
@article{bd692bd6-222d-48e1-aa6b-3a360ba2a97f, abstract = {{<p>Germinal centers (GC) are well-defined areas in lymphoid organs were B cells proliferate and differentiate in response to T-cell-dependent antigens. The GC comprises B cells, follicular dendritic cells, tangible body macrophages, and a low number of CD4<sup>+</sup> T cells. A large portion of these T cells expresses CD57. We have examined the ability of the CD4<sup>+</sup>CD57<sup>+</sup> GC T cells to become activated and to take part in B cell activation processes, These T cells coexpress CD45RO, CD69, CD28, and upon mitogenic stimulation CD25, The cell population was found neither to containe nor to be able to produce any specific mRNA for IL-2, IL-4, and IFN-γ upon activation. Levels of mRNA encoding CD40 ligand was also undetectable under similar conditions. Furthermore, in contrast to ordinary CD4<sup>+</sup> T cells, this population expressing CD57 was unable to induce B cells to Ig production in the presence of pokeweed mitogen or SEA unless IL-2 was added to the cultures, However, despite their apparent lack of function CD4<sup>+</sup>CD57<sup>+</sup> GC T cells were found to rescue GC B cells from cell death in vitro to the same extent as CD4<sup>+</sup>CD57<sup>-</sup> T(h) cells, The phenotypical and functional differences found between these T cells and regular T(h)-cells suggest that they either represent a T cell subset with distinct properties within the GC yet to be determined or that they represent T cells, Irate in the immune response, having lost most of their original functions and capabilities.</p>}}, author = {{Andersson, Eva and Dahlenborg, Katarina and Ohlin, Mats and Borrebaeck, Carl A K and Carlsson, Roland}}, issn = {{0008-8749}}, language = {{eng}}, month = {{05}}, number = {{2}}, pages = {{166--173}}, publisher = {{Elsevier}}, series = {{Cellular Immunology}}, title = {{Immunoglobulin production induced by CD57+ GC-derived helper T cells in vitro requires addition of exogenous IL-2}}, url = {{http://dx.doi.org/10.1006/cimm.1996.0107}}, doi = {{10.1006/cimm.1996.0107}}, volume = {{169}}, year = {{1996}}, }