Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Focal Adhesion Isolation Assay Using ECM-Coated Magnetic Beads

Sturgess, Wesley LU and Swaminathan, Vinay LU (2021) In Methods in molecular biology (Clifton, N.J.) 2217. p.39-44
Abstract

Focal adhesions are force sensitive structures that dynamically alter their composition, protein-protein interactions, and signaling in response to external mechanical stimuli. These dynamic changes are critical for focal adhesion function and are required for cellular mechanosensing. Here, we describe a simple protocol that allows for isolation of the focal adhesion complex from adherent cells in culture in response to different mechanical stimuli applied at adhesion sites. By combining this assay with approaches such as proteomics or western blot analysis, one can study the force-dependent changes in focal adhesion composition, protein-protein interactions and signaling.

Please use this url to cite or link to this publication:
author
and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Focal adhesion composition, Magnetics, Mechanotransduction
in
Methods in molecular biology (Clifton, N.J.)
volume
2217
pages
6 pages
publisher
Springer
external identifiers
  • scopus:85096458885
  • pmid:33215375
ISSN
1940-6029
DOI
10.1007/978-1-0716-0962-0_4
project
Unravelling the mechanoadhesome of migratory cells
language
English
LU publication?
yes
id
bd7cf2b5-7391-4693-91d4-7c1980b731ba
date added to LUP
2020-11-30 09:33:16
date last changed
2024-05-30 01:01:23
@article{bd7cf2b5-7391-4693-91d4-7c1980b731ba,
  abstract     = {{<p>Focal adhesions are force sensitive structures that dynamically alter their composition, protein-protein interactions, and signaling in response to external mechanical stimuli. These dynamic changes are critical for focal adhesion function and are required for cellular mechanosensing. Here, we describe a simple protocol that allows for isolation of the focal adhesion complex from adherent cells in culture in response to different mechanical stimuli applied at adhesion sites. By combining this assay with approaches such as proteomics or western blot analysis, one can study the force-dependent changes in focal adhesion composition, protein-protein interactions and signaling.</p>}},
  author       = {{Sturgess, Wesley and Swaminathan, Vinay}},
  issn         = {{1940-6029}},
  keywords     = {{Focal adhesion composition; Magnetics; Mechanotransduction}},
  language     = {{eng}},
  pages        = {{39--44}},
  publisher    = {{Springer}},
  series       = {{Methods in molecular biology (Clifton, N.J.)}},
  title        = {{Focal Adhesion Isolation Assay Using ECM-Coated Magnetic Beads}},
  url          = {{http://dx.doi.org/10.1007/978-1-0716-0962-0_4}},
  doi          = {{10.1007/978-1-0716-0962-0_4}},
  volume       = {{2217}},
  year         = {{2021}},
}