Focal Adhesion Isolation Assay Using ECM-Coated Magnetic Beads
(2021) In Methods in molecular biology (Clifton, N.J.) 2217. p.39-44- Abstract
Focal adhesions are force sensitive structures that dynamically alter their composition, protein-protein interactions, and signaling in response to external mechanical stimuli. These dynamic changes are critical for focal adhesion function and are required for cellular mechanosensing. Here, we describe a simple protocol that allows for isolation of the focal adhesion complex from adherent cells in culture in response to different mechanical stimuli applied at adhesion sites. By combining this assay with approaches such as proteomics or western blot analysis, one can study the force-dependent changes in focal adhesion composition, protein-protein interactions and signaling.
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https://lup.lub.lu.se/record/bd7cf2b5-7391-4693-91d4-7c1980b731ba
- author
- Sturgess, Wesley LU and Swaminathan, Vinay LU
- organization
- publishing date
- 2021
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Focal adhesion composition, Magnetics, Mechanotransduction
- in
- Methods in molecular biology (Clifton, N.J.)
- volume
- 2217
- pages
- 6 pages
- publisher
- Springer
- external identifiers
-
- pmid:33215375
- scopus:85096458885
- ISSN
- 1940-6029
- DOI
- 10.1007/978-1-0716-0962-0_4
- project
- Unravelling the mechanoadhesome of migratory cells
- language
- English
- LU publication?
- yes
- id
- bd7cf2b5-7391-4693-91d4-7c1980b731ba
- date added to LUP
- 2020-11-30 09:33:16
- date last changed
- 2024-08-08 06:48:35
@article{bd7cf2b5-7391-4693-91d4-7c1980b731ba, abstract = {{<p>Focal adhesions are force sensitive structures that dynamically alter their composition, protein-protein interactions, and signaling in response to external mechanical stimuli. These dynamic changes are critical for focal adhesion function and are required for cellular mechanosensing. Here, we describe a simple protocol that allows for isolation of the focal adhesion complex from adherent cells in culture in response to different mechanical stimuli applied at adhesion sites. By combining this assay with approaches such as proteomics or western blot analysis, one can study the force-dependent changes in focal adhesion composition, protein-protein interactions and signaling.</p>}}, author = {{Sturgess, Wesley and Swaminathan, Vinay}}, issn = {{1940-6029}}, keywords = {{Focal adhesion composition; Magnetics; Mechanotransduction}}, language = {{eng}}, pages = {{39--44}}, publisher = {{Springer}}, series = {{Methods in molecular biology (Clifton, N.J.)}}, title = {{Focal Adhesion Isolation Assay Using ECM-Coated Magnetic Beads}}, url = {{http://dx.doi.org/10.1007/978-1-0716-0962-0_4}}, doi = {{10.1007/978-1-0716-0962-0_4}}, volume = {{2217}}, year = {{2021}}, }