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Identification, expression, and functional analyses of a thylakoid ATP/ADP carrier from Arabidopsis

Thuswaldner, Sophie ; Lagerstedt, Jens O LU ; Rojas-Stütz, Marc ; Bouhidel, Karim ; Declercq, Christophe ; Leborgne-Castel, Nathalie ; Mishra, Arti ; Marty, Francis ; Schoefs, Benoît and Adamska, Iwona , et al. (2007) In Journal of Biological Chemistry 282(12). p.59-8848
Abstract

In plants the chloroplast thylakoid membrane is the site of light-dependent photosynthetic reactions coupled to ATP synthesis. The ability of the plant cell to build and alter this membrane system is essential for efficient photosynthesis. A nucleotide translocator homologous to the bovine mitochondrial ADP/ATP carrier (AAC) was previously found in spinach thylakoids. Here we have identified and characterized a thylakoid ATP/ADP carrier (TAAC) from Arabidopsis.(i) Sequence homology with the bovine AAC and the prediction of chloroplast transit peptides indicated a putative carrier encoded by the At5g01500 gene, as a TAAC. (ii) Transiently expressed TAAC-green fluorescent protein fusion construct was targeted to the chloroplast. Western... (More)

In plants the chloroplast thylakoid membrane is the site of light-dependent photosynthetic reactions coupled to ATP synthesis. The ability of the plant cell to build and alter this membrane system is essential for efficient photosynthesis. A nucleotide translocator homologous to the bovine mitochondrial ADP/ATP carrier (AAC) was previously found in spinach thylakoids. Here we have identified and characterized a thylakoid ATP/ADP carrier (TAAC) from Arabidopsis.(i) Sequence homology with the bovine AAC and the prediction of chloroplast transit peptides indicated a putative carrier encoded by the At5g01500 gene, as a TAAC. (ii) Transiently expressed TAAC-green fluorescent protein fusion construct was targeted to the chloroplast. Western blotting using a peptide-specific antibody together with immunogold electron microscopy revealed a major location of TAAC in the thylakoid membrane. Previous proteomic analyses identified this protein in chloroplast envelope preparations. (iii) Recombinant TAAC protein specifically imports ATP in exchange for ADP across the cytoplasmic membrane of Escherichia coli. Studies on isolated thylakoids from Arabidopsis confirmed these observations. (iv) The lack of TAAC in an Arabidopsis T-DNA insertion mutant caused a 30-40% reduction in the thylakoid ATP transport and metabolism. (v) TAAC is readily expressed in dark-grown Arabidopsis seedlings, and its level remains stable throughout the greening process. Its expression is highest in developing green tissues and in leaves undergoing senescence or abiotic stress. We propose that the TAAC protein supplies ATP for energy-dependent reactions during thylakoid biogenesis and turnover in plants.

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publication status
published
keywords
Amino Acid Sequence, Animals, Antiporters, Arabidopsis, Arabidopsis Proteins, Cattle, Chloroplasts, Escherichia coli, Green Fluorescent Proteins, Models, Molecular, Molecular Sequence Data, Mutation, Oxidative Stress, Protein Conformation, Recombinant Proteins, Sequence Homology, Amino Acid, Thylakoids, Journal Article, Research Support, Non-U.S. Gov't
in
Journal of Biological Chemistry
volume
282
issue
12
pages
59 - 8848
publisher
ASBMB
external identifiers
  • scopus:34247873159
  • pmid:17261580
ISSN
0021-9258
DOI
10.1074/jbc.M609130200
language
English
LU publication?
no
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bda872ae-396f-4a64-8633-9dd5aedce6c5
date added to LUP
2017-10-19 20:10:28
date last changed
2020-09-30 05:12:40
@article{bda872ae-396f-4a64-8633-9dd5aedce6c5,
  abstract     = {<p>In plants the chloroplast thylakoid membrane is the site of light-dependent photosynthetic reactions coupled to ATP synthesis. The ability of the plant cell to build and alter this membrane system is essential for efficient photosynthesis. A nucleotide translocator homologous to the bovine mitochondrial ADP/ATP carrier (AAC) was previously found in spinach thylakoids. Here we have identified and characterized a thylakoid ATP/ADP carrier (TAAC) from Arabidopsis.(i) Sequence homology with the bovine AAC and the prediction of chloroplast transit peptides indicated a putative carrier encoded by the At5g01500 gene, as a TAAC. (ii) Transiently expressed TAAC-green fluorescent protein fusion construct was targeted to the chloroplast. Western blotting using a peptide-specific antibody together with immunogold electron microscopy revealed a major location of TAAC in the thylakoid membrane. Previous proteomic analyses identified this protein in chloroplast envelope preparations. (iii) Recombinant TAAC protein specifically imports ATP in exchange for ADP across the cytoplasmic membrane of Escherichia coli. Studies on isolated thylakoids from Arabidopsis confirmed these observations. (iv) The lack of TAAC in an Arabidopsis T-DNA insertion mutant caused a 30-40% reduction in the thylakoid ATP transport and metabolism. (v) TAAC is readily expressed in dark-grown Arabidopsis seedlings, and its level remains stable throughout the greening process. Its expression is highest in developing green tissues and in leaves undergoing senescence or abiotic stress. We propose that the TAAC protein supplies ATP for energy-dependent reactions during thylakoid biogenesis and turnover in plants.</p>},
  author       = {Thuswaldner, Sophie and Lagerstedt, Jens O and Rojas-Stütz, Marc and Bouhidel, Karim and Declercq, Christophe and Leborgne-Castel, Nathalie and Mishra, Arti and Marty, Francis and Schoefs, Benoît and Adamska, Iwona and Persson, Bengt L and Spetea, Cornelia},
  issn         = {0021-9258},
  language     = {eng},
  month        = {03},
  number       = {12},
  pages        = {59--8848},
  publisher    = {ASBMB},
  series       = {Journal of Biological Chemistry},
  title        = {Identification, expression, and functional analyses of a thylakoid ATP/ADP carrier from Arabidopsis},
  url          = {http://dx.doi.org/10.1074/jbc.M609130200},
  doi          = {10.1074/jbc.M609130200},
  volume       = {282},
  year         = {2007},
}