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Continuous and simultaneous determination of venous blood metabolites

Andersson, Anneli LU ; Chen, Qun LU ; Groop, Leif LU ; Bülow, Leif LU and Xie, Bin LU (2017) In Talanta 171. p.270-274
Abstract

Metabolic syndrome is associated with cardiovascular disease, type 2 diabetes mellitus (T2DM) and prediabetes. Metabolic syndrome is a cluster of interrelated clinical disorders. Difficulties in regulating glucose levels in blood are implicated in many of these disorders. Lactate, another energy metabolite, is produced under anaerobic conditions and can be used to monitor the balance between aerobic and anaerobic metabolism. Tested together, these metabolite levels can provide pro-diagnostic information that improves patient outcomes. Glucose and lactate were determined continuously and simultaneously in whole blood using a dual-channel thermal biosensor device in which one channel employed glucose oxidase for glucose analysis in... (More)

Metabolic syndrome is associated with cardiovascular disease, type 2 diabetes mellitus (T2DM) and prediabetes. Metabolic syndrome is a cluster of interrelated clinical disorders. Difficulties in regulating glucose levels in blood are implicated in many of these disorders. Lactate, another energy metabolite, is produced under anaerobic conditions and can be used to monitor the balance between aerobic and anaerobic metabolism. Tested together, these metabolite levels can provide pro-diagnostic information that improves patient outcomes. Glucose and lactate were determined continuously and simultaneously in whole blood using a dual-channel thermal biosensor device in which one channel employed glucose oxidase for glucose analysis in comparison with lactate oxidase for lactate analysis in the others. No detectable clogging or interference was observed using venous blood samples. The linear detection range for both the glucose and lactate assays was 0.5–45 mM. The sampling rate of up to 24 samples per hour with assay cycle time of 2.5 min was achieved. Comparative analysis between our device and the HemoCue method showed an excellent correlation. The device was stable for hundreds of injections over a period of 45 days. The broad linear range, fast response and detection sensitivity are satisfactory for the clinical requirements, e.g. for diabetic or cardiovascular patients in intensive care units or surgical operation, where the tight control of blood glucose can decrease morbidity or mortality.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Continuous monitoring, Diabetes monitoring, Glucose, Lactate, Thermal biosensor, Whole blood analysis
in
Talanta
volume
171
pages
5 pages
publisher
Elsevier
external identifiers
  • scopus:85018371788
  • wos:000403511500037
ISSN
0039-9140
DOI
10.1016/j.talanta.2017.05.010
language
English
LU publication?
yes
id
bdce341a-15b6-4379-aa76-22c46ebc00f5
date added to LUP
2017-05-17 09:11:53
date last changed
2017-09-18 11:35:58
@article{bdce341a-15b6-4379-aa76-22c46ebc00f5,
  abstract     = {<p>Metabolic syndrome is associated with cardiovascular disease, type 2 diabetes mellitus (T2DM) and prediabetes. Metabolic syndrome is a cluster of interrelated clinical disorders. Difficulties in regulating glucose levels in blood are implicated in many of these disorders. Lactate, another energy metabolite, is produced under anaerobic conditions and can be used to monitor the balance between aerobic and anaerobic metabolism. Tested together, these metabolite levels can provide pro-diagnostic information that improves patient outcomes. Glucose and lactate were determined continuously and simultaneously in whole blood using a dual-channel thermal biosensor device in which one channel employed glucose oxidase for glucose analysis in comparison with lactate oxidase for lactate analysis in the others. No detectable clogging or interference was observed using venous blood samples. The linear detection range for both the glucose and lactate assays was 0.5–45 mM. The sampling rate of up to 24 samples per hour with assay cycle time of 2.5 min was achieved. Comparative analysis between our device and the HemoCue method showed an excellent correlation. The device was stable for hundreds of injections over a period of 45 days. The broad linear range, fast response and detection sensitivity are satisfactory for the clinical requirements, e.g. for diabetic or cardiovascular patients in intensive care units or surgical operation, where the tight control of blood glucose can decrease morbidity or mortality.</p>},
  author       = {Andersson, Anneli and Chen, Qun and Groop, Leif and Bülow, Leif and Xie, Bin},
  issn         = {0039-9140},
  keyword      = {Continuous monitoring,Diabetes monitoring,Glucose,Lactate,Thermal biosensor,Whole blood analysis},
  language     = {eng},
  month        = {08},
  pages        = {270--274},
  publisher    = {Elsevier},
  series       = {Talanta},
  title        = {Continuous and simultaneous determination of venous blood metabolites},
  url          = {http://dx.doi.org/10.1016/j.talanta.2017.05.010},
  volume       = {171},
  year         = {2017},
}