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A pTalpha-negative subpopulation of CD25+ TN thymocytes revealed by a transgenic marker.

Leandersson, Karin LU ; Mårtensson, A ; Mertsching, E ; Winkler, T ; Ceredig, R ; Mårtensson, I-L and Ivars, Fredrik LU (2002) In Scandinavian Journal of Immunology 55(2). p.119-128
Abstract
We have recently generated 5'lambda5-huTAC mice, which express the human CD25 (huTAC) gene under the control of the 5'-flanking region of the mouse lambda5-gene. The huTAC-transgene was expressed in pre-B cells but neither in mature B cells nor in T cells of these mice. In this report we demonstrate that the transgene is also transiently expressed by adult CD25+ CD3-CD4-CD8- (triple negative, TN) thymocytes and in fetal thymocytes. The huTAC+, in contrast to the huTAC- subpopulation of the CD44+CD25+ TN cells, was unexpectedly found not to express the pTalpha-gene. Still the huTAC+CD44+CD25+ TN cells reconstituted the development of both alphabeta and gammadelta lineage cells equally efficiently as the pTalpha-expressing huTAC- fraction,... (More)
We have recently generated 5'lambda5-huTAC mice, which express the human CD25 (huTAC) gene under the control of the 5'-flanking region of the mouse lambda5-gene. The huTAC-transgene was expressed in pre-B cells but neither in mature B cells nor in T cells of these mice. In this report we demonstrate that the transgene is also transiently expressed by adult CD25+ CD3-CD4-CD8- (triple negative, TN) thymocytes and in fetal thymocytes. The huTAC+, in contrast to the huTAC- subpopulation of the CD44+CD25+ TN cells, was unexpectedly found not to express the pTalpha-gene. Still the huTAC+CD44+CD25+ TN cells reconstituted the development of both alphabeta and gammadelta lineage cells equally efficiently as the pTalpha-expressing huTAC- fraction, demonstrating that this pTalpha-negative subpopulation contained precursors for both T-cell lineages. Single cell reverse transcription-polymerase chain reaction (RT-PCR) experiments demonstrated that also in normal mice only a fraction of CD44+CD25+ and CD44-CD25+ TN cells expressed this gene. Taken together, these data indicate that huTAC transgene expression revealed a truly pTalpha-negative fraction of the CD44+CD25+ TN cells. The observation that not all precursors in the CD25+ TN population express the pTalpha-gene has important implications for the understanding of early T-cell development and T-cell lineage commitment. (Less)
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author
; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Antigen, T-Cell, gamma-delta/metabolism, Receptors, Interleukin-2/*genetics/*metabolism, alpha-beta/metabolism, Transgenic, Mice, Membrane Glycoproteins/*genetics, Liver/cytology/immunology, Hematopoietic Stem Cells/immunology, Genetic Markers, Reporter, Genes, Gene Expression, Fetus/cytology/immunology, B-Lymphocytes/immunology, CD44/metabolism, Animal, Antigens, T-Lymphocyte Subsets/*immunology, Thymus Gland/cytology/immunology
in
Scandinavian Journal of Immunology
volume
55
issue
2
pages
119 - 128
publisher
Wiley-Blackwell
external identifiers
  • wos:000173865700002
  • pmid:11896928
  • scopus:0036010568
ISSN
1365-3083
DOI
10.1046/j.1365-3083.2002.01022.x
language
English
LU publication?
yes
id
be0f0117-3ea6-49b2-9aee-e5fa1d096022 (old id 105980)
alternative location
http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11896928&dopt=Abstract
date added to LUP
2016-04-01 15:53:12
date last changed
2022-01-28 07:49:32
@article{be0f0117-3ea6-49b2-9aee-e5fa1d096022,
  abstract     = {{We have recently generated 5'lambda5-huTAC mice, which express the human CD25 (huTAC) gene under the control of the 5'-flanking region of the mouse lambda5-gene. The huTAC-transgene was expressed in pre-B cells but neither in mature B cells nor in T cells of these mice. In this report we demonstrate that the transgene is also transiently expressed by adult CD25+ CD3-CD4-CD8- (triple negative, TN) thymocytes and in fetal thymocytes. The huTAC+, in contrast to the huTAC- subpopulation of the CD44+CD25+ TN cells, was unexpectedly found not to express the pTalpha-gene. Still the huTAC+CD44+CD25+ TN cells reconstituted the development of both alphabeta and gammadelta lineage cells equally efficiently as the pTalpha-expressing huTAC- fraction, demonstrating that this pTalpha-negative subpopulation contained precursors for both T-cell lineages. Single cell reverse transcription-polymerase chain reaction (RT-PCR) experiments demonstrated that also in normal mice only a fraction of CD44+CD25+ and CD44-CD25+ TN cells expressed this gene. Taken together, these data indicate that huTAC transgene expression revealed a truly pTalpha-negative fraction of the CD44+CD25+ TN cells. The observation that not all precursors in the CD25+ TN population express the pTalpha-gene has important implications for the understanding of early T-cell development and T-cell lineage commitment.}},
  author       = {{Leandersson, Karin and Mårtensson, A and Mertsching, E and Winkler, T and Ceredig, R and Mårtensson, I-L and Ivars, Fredrik}},
  issn         = {{1365-3083}},
  keywords     = {{Antigen; T-Cell; gamma-delta/metabolism; Receptors; Interleukin-2/*genetics/*metabolism; alpha-beta/metabolism; Transgenic; Mice; Membrane Glycoproteins/*genetics; Liver/cytology/immunology; Hematopoietic Stem Cells/immunology; Genetic Markers; Reporter; Genes; Gene Expression; Fetus/cytology/immunology; B-Lymphocytes/immunology; CD44/metabolism; Animal; Antigens; T-Lymphocyte Subsets/*immunology; Thymus Gland/cytology/immunology}},
  language     = {{eng}},
  number       = {{2}},
  pages        = {{119--128}},
  publisher    = {{Wiley-Blackwell}},
  series       = {{Scandinavian Journal of Immunology}},
  title        = {{A pTalpha-negative subpopulation of CD25+ TN thymocytes revealed by a transgenic marker.}},
  url          = {{http://dx.doi.org/10.1046/j.1365-3083.2002.01022.x}},
  doi          = {{10.1046/j.1365-3083.2002.01022.x}},
  volume       = {{55}},
  year         = {{2002}},
}