Advanced

Identification of the ligand of Pru p 3, a peach LTP

Cubells-Baeza, Nuria; Gómez-Casado, Cristina LU ; Tordesillas, Leticia; Ramírez-Castillejo, Carmen; Garrido-Arandia, María; González-Melendi, Pablo; Herrero, María; Pacios, Luis F. and Díaz-Perales, Araceli (2017) In Plant Molecular Biology 94(1-2). p.33-44
Abstract

Key message: Pru p 3, a peach LTP, is located in pollinated flower styles and secreting downy hairs, transporting a derivative of camptothecin bound to phytosphingosine. Pru p 3 may inhibit a second pollination and may keep away herbivores until seed maturation. Abstract: The allergen Pru p 3, a peach lipid transfer protein, has been well studied. However, its physiological function remains to be elucidated. Our results showed that Pru p 3 usually carries a lipid ligand that play an essential role in its function in plants. Using ESI-qToF, we observed that the ligand was a derivative of camptothecin binding to phytosphingosine, wich that is inserted into the hydrophobic tunnel of the protein. In addition, the described ligand displayed... (More)

Key message: Pru p 3, a peach LTP, is located in pollinated flower styles and secreting downy hairs, transporting a derivative of camptothecin bound to phytosphingosine. Pru p 3 may inhibit a second pollination and may keep away herbivores until seed maturation. Abstract: The allergen Pru p 3, a peach lipid transfer protein, has been well studied. However, its physiological function remains to be elucidated. Our results showed that Pru p 3 usually carries a lipid ligand that play an essential role in its function in plants. Using ESI-qToF, we observed that the ligand was a derivative of camptothecin binding to phytosphingosine, wich that is inserted into the hydrophobic tunnel of the protein. In addition, the described ligand displayed topoisomerase I activity inhibition and self-fluorescence, both recognized as camptothecin properties. During flower development, the highest expression of Pru p 3 was detected in the styles of pollinated flowers, in contrast to its non-expression in unpollinated pistils, where expression decreased after anthesis. During ripening, the expression of Pru p 3 were observed mainly in peel but not in pulp. In this sense, Pru p 3 protein was also localized in trichomes covering the fruit epidermis.

(Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Camptothecin, Flower development, Fruit development, Lipid transfer protein, Pollination, Pru p 3, Secondary metabolites
in
Plant Molecular Biology
volume
94
issue
1-2
pages
12 pages
publisher
Kluwer
external identifiers
  • scopus:85015257435
  • wos:000401893100003
ISSN
0167-4412
DOI
10.1007/s11103-017-0590-z
language
English
LU publication?
yes
id
be2d508d-75ca-4fb3-afac-69783e99ad25
date added to LUP
2017-03-29 12:15:13
date last changed
2017-09-18 11:34:22
@article{be2d508d-75ca-4fb3-afac-69783e99ad25,
  abstract     = {<p>Key message: Pru p 3, a peach LTP, is located in pollinated flower styles and secreting downy hairs, transporting a derivative of camptothecin bound to phytosphingosine. Pru p 3 may inhibit a second pollination and may keep away herbivores until seed maturation. Abstract: The allergen Pru p 3, a peach lipid transfer protein, has been well studied. However, its physiological function remains to be elucidated. Our results showed that Pru p 3 usually carries a lipid ligand that play an essential role in its function in plants. Using ESI-qToF, we observed that the ligand was a derivative of camptothecin binding to phytosphingosine, wich that is inserted into the hydrophobic tunnel of the protein. In addition, the described ligand displayed topoisomerase I activity inhibition and self-fluorescence, both recognized as camptothecin properties. During flower development, the highest expression of Pru p 3 was detected in the styles of pollinated flowers, in contrast to its non-expression in unpollinated pistils, where expression decreased after anthesis. During ripening, the expression of Pru p 3 were observed mainly in peel but not in pulp. In this sense, Pru p 3 protein was also localized in trichomes covering the fruit epidermis.</p>},
  author       = {Cubells-Baeza, Nuria and Gómez-Casado, Cristina and Tordesillas, Leticia and Ramírez-Castillejo, Carmen and Garrido-Arandia, María and González-Melendi, Pablo and Herrero, María and Pacios, Luis F. and Díaz-Perales, Araceli},
  issn         = {0167-4412},
  keyword      = {Camptothecin,Flower development,Fruit development,Lipid transfer protein,Pollination,Pru p 3,Secondary metabolites},
  language     = {eng},
  number       = {1-2},
  pages        = {33--44},
  publisher    = {Kluwer},
  series       = {Plant Molecular Biology},
  title        = {Identification of the ligand of Pru p 3, a peach LTP},
  url          = {http://dx.doi.org/10.1007/s11103-017-0590-z},
  volume       = {94},
  year         = {2017},
}