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Studies of the release and turnover of a human neutrophil lipocalin

Axelsson, L. LU ; Bergenfeldt, M. LU and Ohlsson, K. (1995) In Scandinavian Journal of Clinical and Laboratory Investigation 55(7). p.577-588
Abstract

A 24-kDa protein was purified from human neutrophil extracts and shown to be the newly discovered neutrophil gelatinase-associated lipocalin (NGAL), based on structural and immunochemical data. A specific enzyme-linked immunosorbent assay (ELISA) was developed for the determination of NGAL in human plasma and tissue fluids. Normal human plasma contains 72 μg 1-1 of NGAL (range 40-109 μg 1-1) in two main forms, monomer and dimer. 35S-methionine metabolic studies of human neutrophils showed that granulocyte macrophagecolony-stimulating factor (GMCSF) stimulated significant synthesis and secretion of NGAL in a dose- and time-dependent fashion. NGAL was rapidly released as monomer and dimer on incubation of... (More)

A 24-kDa protein was purified from human neutrophil extracts and shown to be the newly discovered neutrophil gelatinase-associated lipocalin (NGAL), based on structural and immunochemical data. A specific enzyme-linked immunosorbent assay (ELISA) was developed for the determination of NGAL in human plasma and tissue fluids. Normal human plasma contains 72 μg 1-1 of NGAL (range 40-109 μg 1-1) in two main forms, monomer and dimer. 35S-methionine metabolic studies of human neutrophils showed that granulocyte macrophagecolony-stimulating factor (GMCSF) stimulated significant synthesis and secretion of NGAL in a dose- and time-dependent fashion. NGAL was rapidly released as monomer and dimer on incubation of heparinized whole blood with opsonized yeast, reaching a plateau corresponding to about 35% of total cell content after 30 min. Following intravenous injection of 125-iodine labelled NGAL there was a more rapid initial clearance of the monomeric than of the dimeric form; t1/2 10 min vs. 20 min. During the second phase the two forms cleared at similar rates. Severe acute peritonitis was accompanied by a 10-fold increase in NGAL plasma levels and the NGAL level in peritoneal exudates, which reached about 40 mg 1-1. There was a good linear correlation between the concentrations of NGAL, leucocyte elastase and NP4 (neutrophil proteinase 4 = P3).

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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Cytokines, ELISA, Leucocyte proteinases, Lipocalin, Neutrophil, Peritonitis, Phagocytosis
in
Scandinavian Journal of Clinical and Laboratory Investigation
volume
55
issue
7
pages
12 pages
publisher
Informa Healthcare
external identifiers
  • pmid:8633182
  • scopus:0028849626
ISSN
0036-5513
DOI
10.3109/00365519509110257
language
English
LU publication?
yes
id
be3f037d-9cdd-4bde-a730-dea34527fba7
date added to LUP
2017-03-10 13:27:28
date last changed
2025-01-07 09:26:59
@article{be3f037d-9cdd-4bde-a730-dea34527fba7,
  abstract     = {{<p>A 24-kDa protein was purified from human neutrophil extracts and shown to be the newly discovered neutrophil gelatinase-associated lipocalin (NGAL), based on structural and immunochemical data. A specific enzyme-linked immunosorbent assay (ELISA) was developed for the determination of NGAL in human plasma and tissue fluids. Normal human plasma contains 72 μg 1<sup>-1</sup> of NGAL (range 40-109 μg 1<sup>-1</sup>) in two main forms, monomer and dimer. <sup>35</sup>S-methionine metabolic studies of human neutrophils showed that granulocyte macrophagecolony-stimulating factor (GMCSF) stimulated significant synthesis and secretion of NGAL in a dose- and time-dependent fashion. NGAL was rapidly released as monomer and dimer on incubation of heparinized whole blood with opsonized yeast, reaching a plateau corresponding to about 35% of total cell content after 30 min. Following intravenous injection of <sup>125</sup>-iodine labelled NGAL there was a more rapid initial clearance of the monomeric than of the dimeric form; t<sub>1/2</sub> 10 min vs. 20 min. During the second phase the two forms cleared at similar rates. Severe acute peritonitis was accompanied by a 10-fold increase in NGAL plasma levels and the NGAL level in peritoneal exudates, which reached about 40 mg 1<sup>-1</sup>. There was a good linear correlation between the concentrations of NGAL, leucocyte elastase and NP4 (neutrophil proteinase 4 = P3).</p>}},
  author       = {{Axelsson, L. and Bergenfeldt, M. and Ohlsson, K.}},
  issn         = {{0036-5513}},
  keywords     = {{Cytokines; ELISA; Leucocyte proteinases; Lipocalin; Neutrophil; Peritonitis; Phagocytosis}},
  language     = {{eng}},
  number       = {{7}},
  pages        = {{577--588}},
  publisher    = {{Informa Healthcare}},
  series       = {{Scandinavian Journal of Clinical and Laboratory Investigation}},
  title        = {{Studies of the release and turnover of a human neutrophil lipocalin}},
  url          = {{http://dx.doi.org/10.3109/00365519509110257}},
  doi          = {{10.3109/00365519509110257}},
  volume       = {{55}},
  year         = {{1995}},
}