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Studies of the release and turnover of a human neutrophil lipocalin

Axelsson, L. LU ; Bergenfeldt, M. LU and Ohlsson, K. (1995) In Scandinavian Journal of Clinical and Laboratory Investigation 55(7). p.577-588
Abstract

A 24-kDa protein was purified from human neutrophil extracts and shown to be the newly discovered neutrophil gelatinase-associated lipocalin (NGAL), based on structural and immunochemical data. A specific enzyme-linked immunosorbent assay (ELISA) was developed for the determination of NGAL in human plasma and tissue fluids. Normal human plasma contains 72 μg 1-1 of NGAL (range 40-109 μg 1-1) in two main forms, monomer and dimer. 35S-methionine metabolic studies of human neutrophils showed that granulocyte macrophagecolony-stimulating factor (GMCSF) stimulated significant synthesis and secretion of NGAL in a dose- and time-dependent fashion. NGAL was rapidly released as monomer and dimer on incubation of... (More)

A 24-kDa protein was purified from human neutrophil extracts and shown to be the newly discovered neutrophil gelatinase-associated lipocalin (NGAL), based on structural and immunochemical data. A specific enzyme-linked immunosorbent assay (ELISA) was developed for the determination of NGAL in human plasma and tissue fluids. Normal human plasma contains 72 μg 1-1 of NGAL (range 40-109 μg 1-1) in two main forms, monomer and dimer. 35S-methionine metabolic studies of human neutrophils showed that granulocyte macrophagecolony-stimulating factor (GMCSF) stimulated significant synthesis and secretion of NGAL in a dose- and time-dependent fashion. NGAL was rapidly released as monomer and dimer on incubation of heparinized whole blood with opsonized yeast, reaching a plateau corresponding to about 35% of total cell content after 30 min. Following intravenous injection of 125-iodine labelled NGAL there was a more rapid initial clearance of the monomeric than of the dimeric form; t1/2 10 min vs. 20 min. During the second phase the two forms cleared at similar rates. Severe acute peritonitis was accompanied by a 10-fold increase in NGAL plasma levels and the NGAL level in peritoneal exudates, which reached about 40 mg 1-1. There was a good linear correlation between the concentrations of NGAL, leucocyte elastase and NP4 (neutrophil proteinase 4 = P3). © 1995 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.

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author
organization
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type
Contribution to journal
publication status
published
subject
keywords
Cytokines, ELISA, Leucocyte proteinases, Lipocalin, Neutrophil, Peritonitis, Phagocytosis
in
Scandinavian Journal of Clinical and Laboratory Investigation
volume
55
issue
7
pages
12 pages
publisher
Informa Healthcare
external identifiers
  • scopus:0028849626
ISSN
0036-5513
DOI
10.3109/00365519509110257
language
English
LU publication?
yes
id
be3f037d-9cdd-4bde-a730-dea34527fba7
date added to LUP
2017-03-10 13:27:28
date last changed
2017-05-28 04:58:30
@article{be3f037d-9cdd-4bde-a730-dea34527fba7,
  abstract     = {<p>A 24-kDa protein was purified from human neutrophil extracts and shown to be the newly discovered neutrophil gelatinase-associated lipocalin (NGAL), based on structural and immunochemical data. A specific enzyme-linked immunosorbent assay (ELISA) was developed for the determination of NGAL in human plasma and tissue fluids. Normal human plasma contains 72 μg 1<sup>-1</sup> of NGAL (range 40-109 μg 1<sup>-1</sup>) in two main forms, monomer and dimer. <sup>35</sup>S-methionine metabolic studies of human neutrophils showed that granulocyte macrophagecolony-stimulating factor (GMCSF) stimulated significant synthesis and secretion of NGAL in a dose- and time-dependent fashion. NGAL was rapidly released as monomer and dimer on incubation of heparinized whole blood with opsonized yeast, reaching a plateau corresponding to about 35% of total cell content after 30 min. Following intravenous injection of <sup>125</sup>-iodine labelled NGAL there was a more rapid initial clearance of the monomeric than of the dimeric form; t<sub>1/2</sub> 10 min vs. 20 min. During the second phase the two forms cleared at similar rates. Severe acute peritonitis was accompanied by a 10-fold increase in NGAL plasma levels and the NGAL level in peritoneal exudates, which reached about 40 mg 1<sup>-1</sup>. There was a good linear correlation between the concentrations of NGAL, leucocyte elastase and NP4 (neutrophil proteinase 4 = P3). © 1995 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.</p>},
  author       = {Axelsson, L. and Bergenfeldt, M. and Ohlsson, K.},
  issn         = {0036-5513},
  keyword      = {Cytokines,ELISA,Leucocyte proteinases,Lipocalin,Neutrophil,Peritonitis,Phagocytosis},
  language     = {eng},
  number       = {7},
  pages        = {577--588},
  publisher    = {Informa Healthcare},
  series       = {Scandinavian Journal of Clinical and Laboratory Investigation},
  title        = {Studies of the release and turnover of a human neutrophil lipocalin},
  url          = {http://dx.doi.org/10.3109/00365519509110257},
  volume       = {55},
  year         = {1995},
}