Nulp1, a novel basic helix-loop-helix protein expressed broadly during early embryonic organogenesis and prominently in developing dorsal root ganglia.
(2002) In Cell and Tissue Research 308(3). p.361-370- Abstract
- The basic helix-loop-helix (bHLH) proteins control differentiation and development of a variety of organs. We have isolated the complementary DNA (cDNA) of a novel class of bHLH transcription factors. The previously uncharacterized bHLH messenger RNA (mRNA) was identified by RNA fingerprinting by comparing embryonic and adult mRNA. The reading frame sequence predicts a new class of bHLH family. Northern blotting of embryonic stages demonstrated a 3.2-kb transcript present in several embryonic tissues, including kidney, brain, heart, and lung, in a fashion confirmatory with the RNA-fingerprinting data. In situ hybridization of cryosections detected strong signals in the dorsal root ganglia of 14-day-old mouse embryos (E14). Transient... (More)
- The basic helix-loop-helix (bHLH) proteins control differentiation and development of a variety of organs. We have isolated the complementary DNA (cDNA) of a novel class of bHLH transcription factors. The previously uncharacterized bHLH messenger RNA (mRNA) was identified by RNA fingerprinting by comparing embryonic and adult mRNA. The reading frame sequence predicts a new class of bHLH family. Northern blotting of embryonic stages demonstrated a 3.2-kb transcript present in several embryonic tissues, including kidney, brain, heart, and lung, in a fashion confirmatory with the RNA-fingerprinting data. In situ hybridization of cryosections detected strong signals in the dorsal root ganglia of 14-day-old mouse embryos (E14). Transient transfection of human embryonic kidney cells with Nulp1-EGFP demonstrated nuclear localization. The complex expression pattern and unique protein sequence, including an acidic amino terminal and putative transcription activation domain, suggests that Nulp1 may have a distinct role in embryonic development of many organs, including the adult brain. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/109185
- author
- Olsson, Magnus ; Durbeej-Hjalt, Madeleine LU ; Ekblom, Peter LU and Hjalt, Tord LU
- organization
- publishing date
- 2002
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Cell and Tissue Research
- volume
- 308
- issue
- 3
- pages
- 361 - 370
- publisher
- Springer
- external identifiers
-
- pmid:12107429
- wos:000176900700003
- scopus:0035989137
- ISSN
- 1432-0878
- DOI
- 10.1007/s00441-002-0544-9
- language
- English
- LU publication?
- yes
- id
- be5cb6ba-34b0-4dfe-a4a6-483807eb65c7 (old id 109185)
- alternative location
- http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12107429&dopt=Abstract
- date added to LUP
- 2016-04-01 12:07:54
- date last changed
- 2022-02-26 02:19:06
@article{be5cb6ba-34b0-4dfe-a4a6-483807eb65c7, abstract = {{The basic helix-loop-helix (bHLH) proteins control differentiation and development of a variety of organs. We have isolated the complementary DNA (cDNA) of a novel class of bHLH transcription factors. The previously uncharacterized bHLH messenger RNA (mRNA) was identified by RNA fingerprinting by comparing embryonic and adult mRNA. The reading frame sequence predicts a new class of bHLH family. Northern blotting of embryonic stages demonstrated a 3.2-kb transcript present in several embryonic tissues, including kidney, brain, heart, and lung, in a fashion confirmatory with the RNA-fingerprinting data. In situ hybridization of cryosections detected strong signals in the dorsal root ganglia of 14-day-old mouse embryos (E14). Transient transfection of human embryonic kidney cells with Nulp1-EGFP demonstrated nuclear localization. The complex expression pattern and unique protein sequence, including an acidic amino terminal and putative transcription activation domain, suggests that Nulp1 may have a distinct role in embryonic development of many organs, including the adult brain.}}, author = {{Olsson, Magnus and Durbeej-Hjalt, Madeleine and Ekblom, Peter and Hjalt, Tord}}, issn = {{1432-0878}}, language = {{eng}}, number = {{3}}, pages = {{361--370}}, publisher = {{Springer}}, series = {{Cell and Tissue Research}}, title = {{Nulp1, a novel basic helix-loop-helix protein expressed broadly during early embryonic organogenesis and prominently in developing dorsal root ganglia.}}, url = {{http://dx.doi.org/10.1007/s00441-002-0544-9}}, doi = {{10.1007/s00441-002-0544-9}}, volume = {{308}}, year = {{2002}}, }