Programming human cell fate : overcoming challenges and unlocking potential through technological breakthroughs
Lin, Hsiu Chuan ; Makhlouf, Aly ; Echegaray, Camila Vazquez LU
; Zawada, Dorota
and Simões, Filipa
(2023)
In Development (Cambridge)
150(24).
- Abstract
In recent years, there have been notable advancements in the ability to programme human cell identity, enabling us to design and manipulate cell function in a Petri dish. However, current protocols for generating target cell types often lack efficiency and precision, resulting in engineered cells that do not fully replicate the desired identity or functional output. This applies to different methods of cell programming, which face similar challenges that hinder progress and delay the achievement of a more favourable outcome. However, recent technological and analytical breakthroughs have provided us with unprecedented opportunities to advance the way we programme cell fate. The Company of Biologists' 2023 workshop on 'Novel Technologies... (More)
In recent years, there have been notable advancements in the ability to programme human cell identity, enabling us to design and manipulate cell function in a Petri dish. However, current protocols for generating target cell types often lack efficiency and precision, resulting in engineered cells that do not fully replicate the desired identity or functional output. This applies to different methods of cell programming, which face similar challenges that hinder progress and delay the achievement of a more favourable outcome. However, recent technological and analytical breakthroughs have provided us with unprecedented opportunities to advance the way we programme cell fate. The Company of Biologists' 2023 workshop on 'Novel Technologies for Programming Human Cell Fate' brought together experts in human cell fate engineering and experts in single-cell genomics, manipulation and characterisation of cells on a single (sub)cellular level. Here, we summarise the main points that emerged during the workshop's themed discussions. Furthermore, we provide specific examples highlighting the current state of the field as well as its trajectory, offering insights into the potential outcomes resulting from the application of these breakthrough technologies in precisely engineering the identity and function of clinically valuable human cells.
(Less)
- author
- Lin, Hsiu Chuan
; Makhlouf, Aly
; Echegaray, Camila Vazquez
LU
; Zawada, Dorota
and Simões, Filipa
- organization
- publishing date
- 2023-12
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Cell programming, Genomic engineering, Human cell fate, Reprogramming, Synthetic biology
- in
- Development (Cambridge)
- volume
- 150
- issue
- 24
- publisher
- The Company of Biologists Ltd
- external identifiers
-
- pmid:38078653
- scopus:85179644036
- ISSN
- 0950-1991
- DOI
- 10.1242/dev.202300
- language
- English
- LU publication?
- yes
- id
- be607e9f-03ee-4b7d-8a9d-4baf713d2f69
- date added to LUP
- 2024-01-02 15:05:46
- date last changed
- 2024-04-17 14:22:27
@article{be607e9f-03ee-4b7d-8a9d-4baf713d2f69,
abstract = {{<p>In recent years, there have been notable advancements in the ability to programme human cell identity, enabling us to design and manipulate cell function in a Petri dish. However, current protocols for generating target cell types often lack efficiency and precision, resulting in engineered cells that do not fully replicate the desired identity or functional output. This applies to different methods of cell programming, which face similar challenges that hinder progress and delay the achievement of a more favourable outcome. However, recent technological and analytical breakthroughs have provided us with unprecedented opportunities to advance the way we programme cell fate. The Company of Biologists' 2023 workshop on 'Novel Technologies for Programming Human Cell Fate' brought together experts in human cell fate engineering and experts in single-cell genomics, manipulation and characterisation of cells on a single (sub)cellular level. Here, we summarise the main points that emerged during the workshop's themed discussions. Furthermore, we provide specific examples highlighting the current state of the field as well as its trajectory, offering insights into the potential outcomes resulting from the application of these breakthrough technologies in precisely engineering the identity and function of clinically valuable human cells.</p>}},
author = {{Lin, Hsiu Chuan and Makhlouf, Aly and Echegaray, Camila Vazquez and Zawada, Dorota and Simões, Filipa}},
issn = {{0950-1991}},
keywords = {{Cell programming; Genomic engineering; Human cell fate; Reprogramming; Synthetic biology}},
language = {{eng}},
number = {{24}},
publisher = {{The Company of Biologists Ltd}},
series = {{Development (Cambridge)}},
title = {{Programming human cell fate : overcoming challenges and unlocking potential through technological breakthroughs}},
url = {{http://dx.doi.org/10.1242/dev.202300}},
doi = {{10.1242/dev.202300}},
volume = {{150}},
year = {{2023}},
}