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Involvement of sensory nerves in vasodilator responses to acetylcholine and potassium ions in rat hepatic artery

Högestätt, Edward D. LU ; Johansson, Rebecka LU ; Andersson, David A. LU and Zygmunt, Peter M. LU (2000) In British Journal of Pharmacology 130(1). p.27-32
Abstract

In the presence of ouabain (1 mM), acetylcholine and KCl (5 mM) evoked endothelium-independent relaxations in rat hepatic arteries. Treatment with capsaicin (10 μM), scopolamine (1 μM) or CGRP8-37 (3 μM) prevented these relaxations. Acetylcholine-induced relaxations in intact arterial segments in the presence of indomethacin (10 μM) and N(G)-nitro-L-arginine (0.3 mM) were only partially inhibited by ouabain plus BaCl2 (30 μM). However, ouabain plus BaCl2 almost abolished such relaxations in capsaicin-pre-treated preparations. In arteries without endothelium, the neurosecretagogue α-latrotoxin (1 nM) induced complete relaxations, which were abolished by CGRP8-37 or pre-treatment with capsaicin.... (More)

In the presence of ouabain (1 mM), acetylcholine and KCl (5 mM) evoked endothelium-independent relaxations in rat hepatic arteries. Treatment with capsaicin (10 μM), scopolamine (1 μM) or CGRP8-37 (3 μM) prevented these relaxations. Acetylcholine-induced relaxations in intact arterial segments in the presence of indomethacin (10 μM) and N(G)-nitro-L-arginine (0.3 mM) were only partially inhibited by ouabain plus BaCl2 (30 μM). However, ouabain plus BaCl2 almost abolished such relaxations in capsaicin-pre-treated preparations. In arteries without endothelium, the neurosecretagogue α-latrotoxin (1 nM) induced complete relaxations, which were abolished by CGRP8-37 or pre-treatment with capsaicin. α-Latrotoxin also induced a smooth muscle hyperpolarization (12 ± 2 mV), which was abolished by CGRP8-37. The ability of ouabain to disclose a CGRP-mediated neurogenic relaxation must be considered when this agent is used as a pharmacological tool. The results further suggest that CGRP is a nerve-derived hyperpolarizing factor in the rat hepatic artery.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Endothelium-derived hyperpolarizing factor, Endothelium-derived relaxing factors, Hyperpolarization, Membrane potential, Nitric oxide, Potassium channels, Vascular endothelium
in
British Journal of Pharmacology
volume
130
issue
1
pages
6 pages
publisher
The British Pharmacological Society
external identifiers
  • scopus:0034099266
ISSN
0007-1188
DOI
10.1038/sj.bjp.0703258
language
English
LU publication?
yes
id
be9f1794-f1ae-4a42-bdd6-8be1bd33eafd
date added to LUP
2019-05-31 21:35:39
date last changed
2019-06-18 02:19:00
@article{be9f1794-f1ae-4a42-bdd6-8be1bd33eafd,
  abstract     = {<p>In the presence of ouabain (1 mM), acetylcholine and KCl (5 mM) evoked endothelium-independent relaxations in rat hepatic arteries. Treatment with capsaicin (10 μM), scopolamine (1 μM) or CGRP<sub>8-37</sub> (3 μM) prevented these relaxations. Acetylcholine-induced relaxations in intact arterial segments in the presence of indomethacin (10 μM) and N(G)-nitro-L-arginine (0.3 mM) were only partially inhibited by ouabain plus BaCl<sub>2</sub> (30 μM). However, ouabain plus BaCl<sub>2</sub> almost abolished such relaxations in capsaicin-pre-treated preparations. In arteries without endothelium, the neurosecretagogue α-latrotoxin (1 nM) induced complete relaxations, which were abolished by CGRP<sub>8-37</sub> or pre-treatment with capsaicin. α-Latrotoxin also induced a smooth muscle hyperpolarization (12 ± 2 mV), which was abolished by CGRP<sub>8-37</sub>. The ability of ouabain to disclose a CGRP-mediated neurogenic relaxation must be considered when this agent is used as a pharmacological tool. The results further suggest that CGRP is a nerve-derived hyperpolarizing factor in the rat hepatic artery.</p>},
  author       = {Högestätt, Edward D. and Johansson, Rebecka and Andersson, David A. and Zygmunt, Peter M.},
  issn         = {0007-1188},
  keyword      = {Endothelium-derived hyperpolarizing factor,Endothelium-derived relaxing factors,Hyperpolarization,Membrane potential,Nitric oxide,Potassium channels,Vascular endothelium},
  language     = {eng},
  month        = {01},
  number       = {1},
  pages        = {27--32},
  publisher    = {The British Pharmacological Society},
  series       = {British Journal of Pharmacology},
  title        = {Involvement of sensory nerves in vasodilator responses to acetylcholine and potassium ions in rat hepatic artery},
  url          = {http://dx.doi.org/10.1038/sj.bjp.0703258},
  volume       = {130},
  year         = {2000},
}