Aspects of estimating parameter dependencies in a detailed chromatography model based on frontal experiments
(2006) In Process Biochemistry 41(8). p.1812-1821- Abstract
- A methodology for estimation of the dependency on flow rate and bead size of the axial dispersion coefficient and the film mass transfer coefficient in a detailed model for chromatographic processes is proposed. The model describes the concentration of the solute in the mobile phase and considers external/internal mass transfer resistance. The flow rate dependency of the mixing effect in the external volume is also considered. The unknown model parameters estimated are the bed void, the axial dispersion coefficient, the liquid film mass transfer coefficient, the effective diffusion coefficient and the apparent bead porosity. All the parameters in the model and the parameter dependencies were subsequently determined in three classes of... (More)
- A methodology for estimation of the dependency on flow rate and bead size of the axial dispersion coefficient and the film mass transfer coefficient in a detailed model for chromatographic processes is proposed. The model describes the concentration of the solute in the mobile phase and considers external/internal mass transfer resistance. The flow rate dependency of the mixing effect in the external volume is also considered. The unknown model parameters estimated are the bed void, the axial dispersion coefficient, the liquid film mass transfer coefficient, the effective diffusion coefficient and the apparent bead porosity. All the parameters in the model and the parameter dependencies were subsequently determined in three classes of experiments, i.e. the external mixing behaviour, the mobile phase behaviour and the stationary phase behaviour. The estimates are based on the sum of the least squares of the residuals between the experimental breakthrough curves and the model response. The methodology is exemplified by frontal experiments with blue dextran and bovine serum albumin in a well-defined column set-up. The results show that it is possible to estimate the unknown physical parameters in the detailed model, and their dependencies on flow rate and bead size, using the proposed methodology. (c) 2006 Elsevier Ltd. All rights reserved. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/405850
- author
- Persson, P ; Gustavsson, Per-Erik LU ; Zacchi, Guido LU and Nilsson, Bernt LU
- organization
- publishing date
- 2006
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- mass transfer resistance, computer simulation, chromatography modelling, parameter estimation, parameter dependencies
- in
- Process Biochemistry
- volume
- 41
- issue
- 8
- pages
- 1812 - 1821
- publisher
- Elsevier
- external identifiers
-
- wos:000238398000015
- scopus:33646802922
- ISSN
- 1873-3298
- DOI
- 10.1016/j.procbio.2006.03.030
- language
- English
- LU publication?
- yes
- id
- bf0018f6-de60-4b3a-a0ee-ea0c9c669f64 (old id 405850)
- date added to LUP
- 2016-04-01 12:30:32
- date last changed
- 2023-09-02 10:10:33
@article{bf0018f6-de60-4b3a-a0ee-ea0c9c669f64,
abstract = {{A methodology for estimation of the dependency on flow rate and bead size of the axial dispersion coefficient and the film mass transfer coefficient in a detailed model for chromatographic processes is proposed. The model describes the concentration of the solute in the mobile phase and considers external/internal mass transfer resistance. The flow rate dependency of the mixing effect in the external volume is also considered. The unknown model parameters estimated are the bed void, the axial dispersion coefficient, the liquid film mass transfer coefficient, the effective diffusion coefficient and the apparent bead porosity. All the parameters in the model and the parameter dependencies were subsequently determined in three classes of experiments, i.e. the external mixing behaviour, the mobile phase behaviour and the stationary phase behaviour. The estimates are based on the sum of the least squares of the residuals between the experimental breakthrough curves and the model response. The methodology is exemplified by frontal experiments with blue dextran and bovine serum albumin in a well-defined column set-up. The results show that it is possible to estimate the unknown physical parameters in the detailed model, and their dependencies on flow rate and bead size, using the proposed methodology. (c) 2006 Elsevier Ltd. All rights reserved.}},
author = {{Persson, P and Gustavsson, Per-Erik and Zacchi, Guido and Nilsson, Bernt}},
issn = {{1873-3298}},
keywords = {{mass transfer resistance; computer simulation; chromatography modelling; parameter estimation; parameter dependencies}},
language = {{eng}},
number = {{8}},
pages = {{1812--1821}},
publisher = {{Elsevier}},
series = {{Process Biochemistry}},
title = {{Aspects of estimating parameter dependencies in a detailed chromatography model based on frontal experiments}},
url = {{http://dx.doi.org/10.1016/j.procbio.2006.03.030}},
doi = {{10.1016/j.procbio.2006.03.030}},
volume = {{41}},
year = {{2006}},
}