Characterization of an alternative transcript of the human CREB3L2 gene.
(2010) In Oncology Reports 24(5). p.1133-1139- Abstract
- CREB3L2, a member of the CREB3 family of transcription factors, spans >120 kbp and is composed of 12 exons. We characterized a widely expressed transcript of CREB3L2 generated by an intronic polyadenylation site in intron 4 of the gene. It could be translated to a CREB3L2 variant which is localized both in the nucleus and the endoplasmatic reticulum. The protein retains the N-terminal transactivation domain but lacks the DNA-binding domain, the transmembrane domain and the C-terminal part. Experiments using a GAL4 DNA-binding domain fusion model showed that the transcript is a transactivator but it cannot exert its function through the CRE and ATF6 binding sites and has little effect on the GRP78 promoter. Whether this transcript has a... (More)
- CREB3L2, a member of the CREB3 family of transcription factors, spans >120 kbp and is composed of 12 exons. We characterized a widely expressed transcript of CREB3L2 generated by an intronic polyadenylation site in intron 4 of the gene. It could be translated to a CREB3L2 variant which is localized both in the nucleus and the endoplasmatic reticulum. The protein retains the N-terminal transactivation domain but lacks the DNA-binding domain, the transmembrane domain and the C-terminal part. Experiments using a GAL4 DNA-binding domain fusion model showed that the transcript is a transactivator but it cannot exert its function through the CRE and ATF6 binding sites and has little effect on the GRP78 promoter. Whether this transcript has a cellular function or is targeted for degradation by nonsense-mediated RNA decay system of RNA surveillance is currently unknown. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1687677
- author
- Panagopoulos, Ioannis LU ; Monsef, Nastaran LU ; Collin, Anna LU and Mertens, Fredrik LU
- organization
- publishing date
- 2010
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Oncology Reports
- volume
- 24
- issue
- 5
- pages
- 1133 - 1139
- publisher
- Spandidos Publications
- external identifiers
-
- wos:000283112200003
- pmid:20878102
- scopus:78149305119
- ISSN
- 1791-2431
- DOI
- 10.3892/or_00000964
- language
- English
- LU publication?
- yes
- id
- bf07991f-e9d3-4d6d-b176-478971417b0a (old id 1687677)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/20878102?dopt=Abstract
- date added to LUP
- 2016-04-04 09:15:38
- date last changed
- 2022-01-29 17:00:46
@article{bf07991f-e9d3-4d6d-b176-478971417b0a,
abstract = {{CREB3L2, a member of the CREB3 family of transcription factors, spans >120 kbp and is composed of 12 exons. We characterized a widely expressed transcript of CREB3L2 generated by an intronic polyadenylation site in intron 4 of the gene. It could be translated to a CREB3L2 variant which is localized both in the nucleus and the endoplasmatic reticulum. The protein retains the N-terminal transactivation domain but lacks the DNA-binding domain, the transmembrane domain and the C-terminal part. Experiments using a GAL4 DNA-binding domain fusion model showed that the transcript is a transactivator but it cannot exert its function through the CRE and ATF6 binding sites and has little effect on the GRP78 promoter. Whether this transcript has a cellular function or is targeted for degradation by nonsense-mediated RNA decay system of RNA surveillance is currently unknown.}},
author = {{Panagopoulos, Ioannis and Monsef, Nastaran and Collin, Anna and Mertens, Fredrik}},
issn = {{1791-2431}},
language = {{eng}},
number = {{5}},
pages = {{1133--1139}},
publisher = {{Spandidos Publications}},
series = {{Oncology Reports}},
title = {{Characterization of an alternative transcript of the human CREB3L2 gene.}},
url = {{http://dx.doi.org/10.3892/or_00000964}},
doi = {{10.3892/or_00000964}},
volume = {{24}},
year = {{2010}},
}