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Common skin bacteria protect their host from oxidative stress through secreted antioxidant RoxP

Andersson, Tilde LU ; Ertürk Bergdahl, Gizem LU ; Saleh, Karim LU ; Magnúsdóttir, Helga; Stødkilde, Kristian; Andersen, Christian Brix Folsted; Lundqvist, Katarina LU ; Jensen, Anders; Brüggemann, Holger and Lood, Rolf LU (2019) In Scientific Reports 9.
Abstract

Cutibacterium acnes is an abundant skin commensal with several proposed mutualistic functions. A protein with strong antioxidant activity was recently identified from the C. acnes secretome. This protein, termed RoxP, facilitated aerobic bacterial growth in vitro and ex vivo. As reducing events naturally occurred outside of the bacterial cell, it was further hypothesized that RoxP could also serve to modulate redox status of human skin. The biological function of RoxP was here assessed in vitro and in vivo, through oxidatively stressed cell cultures and through protein quantification from skin affected by oxidative disease (actinic keratosis and basal cell carcinoma), respectively. 16S rDNA amplicon deep sequencing and single locus... (More)

Cutibacterium acnes is an abundant skin commensal with several proposed mutualistic functions. A protein with strong antioxidant activity was recently identified from the C. acnes secretome. This protein, termed RoxP, facilitated aerobic bacterial growth in vitro and ex vivo. As reducing events naturally occurred outside of the bacterial cell, it was further hypothesized that RoxP could also serve to modulate redox status of human skin. The biological function of RoxP was here assessed in vitro and in vivo, through oxidatively stressed cell cultures and through protein quantification from skin affected by oxidative disease (actinic keratosis and basal cell carcinoma), respectively. 16S rDNA amplicon deep sequencing and single locus sequence typing was used to correlate bacterial prevalence to cutaneous RoxP abundances. We show that RoxP positively influence the viability of monocytes and keratinocytes exposed to oxidative stress, and that a congruent concentration decline of RoxP can be observed in skin affected by oxidative disease. Basal cell carcinoma was moreover associated with microbial dysbiosis, characterized by reduced C. acnes prevalence. C. acnes's secretion of RoxP, an exogenous but naturally occurring antioxidant on human skin, is likely to positively influence the human host. Results furthermore attest to its prospective usability as a biopharmaceutical.

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author
organization
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Contribution to journal
publication status
published
subject
in
Scientific Reports
volume
9
publisher
Nature Publishing Group
external identifiers
  • scopus:85062586061
ISSN
2045-2322
DOI
10.1038/s41598-019-40471-3
language
English
LU publication?
yes
id
bf3392d6-f131-4bf2-8f52-1abfa34578ba
date added to LUP
2019-03-11 16:35:55
date last changed
2019-04-10 04:20:45
@article{bf3392d6-f131-4bf2-8f52-1abfa34578ba,
  abstract     = {<p>Cutibacterium acnes is an abundant skin commensal with several proposed mutualistic functions. A protein with strong antioxidant activity was recently identified from the C. acnes secretome. This protein, termed RoxP, facilitated aerobic bacterial growth in vitro and ex vivo. As reducing events naturally occurred outside of the bacterial cell, it was further hypothesized that RoxP could also serve to modulate redox status of human skin. The biological function of RoxP was here assessed in vitro and in vivo, through oxidatively stressed cell cultures and through protein quantification from skin affected by oxidative disease (actinic keratosis and basal cell carcinoma), respectively. 16S rDNA amplicon deep sequencing and single locus sequence typing was used to correlate bacterial prevalence to cutaneous RoxP abundances. We show that RoxP positively influence the viability of monocytes and keratinocytes exposed to oxidative stress, and that a congruent concentration decline of RoxP can be observed in skin affected by oxidative disease. Basal cell carcinoma was moreover associated with microbial dysbiosis, characterized by reduced C. acnes prevalence. C. acnes's secretion of RoxP, an exogenous but naturally occurring antioxidant on human skin, is likely to positively influence the human host. Results furthermore attest to its prospective usability as a biopharmaceutical.</p>},
  articleno    = {3596},
  author       = {Andersson, Tilde and Ertürk Bergdahl, Gizem and Saleh, Karim and Magnúsdóttir, Helga and Stødkilde, Kristian and Andersen, Christian Brix Folsted and Lundqvist, Katarina and Jensen, Anders and Brüggemann, Holger and Lood, Rolf},
  issn         = {2045-2322},
  language     = {eng},
  month        = {03},
  publisher    = {Nature Publishing Group},
  series       = {Scientific Reports},
  title        = {Common skin bacteria protect their host from oxidative stress through secreted antioxidant RoxP},
  url          = {http://dx.doi.org/10.1038/s41598-019-40471-3},
  volume       = {9},
  year         = {2019},
}