Advanced

High titer ethanol production from rice straw via solid-state simultaneous saccharification and fermentation by Mucor indicus at low enzyme loading

Molaverdi, Maryam LU ; Karimi, Keikhosro; Mirmohamadsadeghi, Safoora and Galbe, Mats LU (2019) In Energy Conversion and Management 182. p.520-529
Abstract

High titer ethanol production from rice straw using Mucor indicus fungus was investigated through the solid-state simultaneous saccharification and fermentation (SSSF) process. The straw was pretreated with 0.5 M sodium carbonate solution for 3, 5, and 10 h to improve the efficiency of the process. Effects of the pretreatment on the composition, structural morphology, cellulose crystallinity, swelling capacity, and buffering capacity of the straw were studied. Moreover, the effects of SSSF reaction time, enzyme loading, and solid loading on glucose and ethanol production were investigated. Additionally, the nutritional value of the residue from the SSSF process, as an animal feedstock, was determined in terms of lipid and protein... (More)

High titer ethanol production from rice straw using Mucor indicus fungus was investigated through the solid-state simultaneous saccharification and fermentation (SSSF) process. The straw was pretreated with 0.5 M sodium carbonate solution for 3, 5, and 10 h to improve the efficiency of the process. Effects of the pretreatment on the composition, structural morphology, cellulose crystallinity, swelling capacity, and buffering capacity of the straw were studied. Moreover, the effects of SSSF reaction time, enzyme loading, and solid loading on glucose and ethanol production were investigated. Additionally, the nutritional value of the residue from the SSSF process, as an animal feedstock, was determined in terms of lipid and protein contents. The highest total sugar concentration was 89.2 g/L, obtained from the straw pretreated for 10 h after hydrolysis with 10 FPU/g straw at 15% (w/w) solid loading. Total sugars concentration was not significantly improved by increasing the pretreatment time at low enzyme loadings, whereas it was significantly improved at high enzyme loadings. The highest ethanol concentration and SSSF yield were 99.4 g/L and 89.5% (71.8% based on the raw material), achieved from the straw pretreated for 10 h through the 72-h SSSF at 30% and 15% solid loading, respectively, using 20 FPU/g straw. The low enzyme loadings of 2.5 and 5 FPU/g straw yielded ethanol with concentrations as high as 66.3 and 90.9 g/L, respectively, after 120-h fermentation at 30% solid loading from the straw pretreated for 5 h.

(Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Dry fermentation, Fungi, High solid loading, Lignocellulose, SSSF
in
Energy Conversion and Management
volume
182
pages
10 pages
publisher
Elsevier
external identifiers
  • scopus:85059534367
ISSN
0196-8904
DOI
10.1016/j.enconman.2018.12.078
language
English
LU publication?
yes
id
bf3bbf02-7bdd-4cce-b94c-1414382bb075
date added to LUP
2019-01-17 12:22:02
date last changed
2019-04-23 04:44:43
@article{bf3bbf02-7bdd-4cce-b94c-1414382bb075,
  abstract     = {<p>High titer ethanol production from rice straw using Mucor indicus fungus was investigated through the solid-state simultaneous saccharification and fermentation (SSSF) process. The straw was pretreated with 0.5 M sodium carbonate solution for 3, 5, and 10 h to improve the efficiency of the process. Effects of the pretreatment on the composition, structural morphology, cellulose crystallinity, swelling capacity, and buffering capacity of the straw were studied. Moreover, the effects of SSSF reaction time, enzyme loading, and solid loading on glucose and ethanol production were investigated. Additionally, the nutritional value of the residue from the SSSF process, as an animal feedstock, was determined in terms of lipid and protein contents. The highest total sugar concentration was 89.2 g/L, obtained from the straw pretreated for 10 h after hydrolysis with 10 FPU/g straw at 15% (w/w) solid loading. Total sugars concentration was not significantly improved by increasing the pretreatment time at low enzyme loadings, whereas it was significantly improved at high enzyme loadings. The highest ethanol concentration and SSSF yield were 99.4 g/L and 89.5% (71.8% based on the raw material), achieved from the straw pretreated for 10 h through the 72-h SSSF at 30% and 15% solid loading, respectively, using 20 FPU/g straw. The low enzyme loadings of 2.5 and 5 FPU/g straw yielded ethanol with concentrations as high as 66.3 and 90.9 g/L, respectively, after 120-h fermentation at 30% solid loading from the straw pretreated for 5 h.</p>},
  author       = {Molaverdi, Maryam and Karimi, Keikhosro and Mirmohamadsadeghi, Safoora and Galbe, Mats},
  issn         = {0196-8904},
  keyword      = {Dry fermentation,Fungi,High solid loading,Lignocellulose,SSSF},
  language     = {eng},
  pages        = {520--529},
  publisher    = {Elsevier},
  series       = {Energy Conversion and Management},
  title        = {High titer ethanol production from rice straw via solid-state simultaneous saccharification and fermentation by Mucor indicus at low enzyme loading},
  url          = {http://dx.doi.org/10.1016/j.enconman.2018.12.078},
  volume       = {182},
  year         = {2019},
}