Microbial acidification by N, S, Fe and Mn oxidation as a key mechanism for deterioration of subsea tunnel sprayed concrete
(2024) In Scientific Reports 14(1).- Abstract
The deterioration of fibre-reinforced sprayed concrete was studied in the Oslofjord subsea tunnel (Norway). At sites with intrusion of saline groundwater resulting in biofilm growth, the concrete exhibited significant concrete deterioration and steel fibre corrosion. Using amplicon sequencing and shotgun metagenomics, the microbial taxa and surveyed potential microbial mechanisms of concrete degradation at two sites over five years were identified. The concrete beneath the biofilm was investigated with polarised light microscopy, scanning electron microscopy and X-ray diffraction. The oxic environment in the tunnel favoured aerobic oxidation processes in nitrogen, sulfur and metal biogeochemical cycling as evidenced by large abundances... (More)
The deterioration of fibre-reinforced sprayed concrete was studied in the Oslofjord subsea tunnel (Norway). At sites with intrusion of saline groundwater resulting in biofilm growth, the concrete exhibited significant concrete deterioration and steel fibre corrosion. Using amplicon sequencing and shotgun metagenomics, the microbial taxa and surveyed potential microbial mechanisms of concrete degradation at two sites over five years were identified. The concrete beneath the biofilm was investigated with polarised light microscopy, scanning electron microscopy and X-ray diffraction. The oxic environment in the tunnel favoured aerobic oxidation processes in nitrogen, sulfur and metal biogeochemical cycling as evidenced by large abundances of metagenome-assembled genomes (MAGs) with potential for oxidation of nitrogen, sulfur, manganese and iron, observed mild acidification of the concrete, and the presence of manganese- and iron oxides. These results suggest that autotrophic microbial populations involved in the cycling of several elements contributed to the corrosion of steel fibres and acidification causing concrete deterioration.
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- author
- Karačić, Sabina ; Suarez, Carolina LU ; Hagelia, Per ; Persson, Frank ; Modin, Oskar ; Martins, Paula Dalcin and Wilén, Britt Marie
- organization
- publishing date
- 2024-12
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Amplicon sequencing, Biodeterioration, Biofilm community, Fibre-reinforced sprayed concrete; subsea tunnels, Metagenomics
- in
- Scientific Reports
- volume
- 14
- issue
- 1
- article number
- 22742
- publisher
- Nature Publishing Group
- external identifiers
-
- pmid:39349736
- scopus:85205447335
- ISSN
- 2045-2322
- DOI
- 10.1038/s41598-024-73911-w
- language
- English
- LU publication?
- yes
- id
- bf9e5cac-f444-48c0-a2ea-dfee3f31e1e0
- date added to LUP
- 2024-11-27 17:45:26
- date last changed
- 2025-07-10 12:28:26
@article{bf9e5cac-f444-48c0-a2ea-dfee3f31e1e0,
abstract = {{<p>The deterioration of fibre-reinforced sprayed concrete was studied in the Oslofjord subsea tunnel (Norway). At sites with intrusion of saline groundwater resulting in biofilm growth, the concrete exhibited significant concrete deterioration and steel fibre corrosion. Using amplicon sequencing and shotgun metagenomics, the microbial taxa and surveyed potential microbial mechanisms of concrete degradation at two sites over five years were identified. The concrete beneath the biofilm was investigated with polarised light microscopy, scanning electron microscopy and X-ray diffraction. The oxic environment in the tunnel favoured aerobic oxidation processes in nitrogen, sulfur and metal biogeochemical cycling as evidenced by large abundances of metagenome-assembled genomes (MAGs) with potential for oxidation of nitrogen, sulfur, manganese and iron, observed mild acidification of the concrete, and the presence of manganese- and iron oxides. These results suggest that autotrophic microbial populations involved in the cycling of several elements contributed to the corrosion of steel fibres and acidification causing concrete deterioration.</p>}},
author = {{Karačić, Sabina and Suarez, Carolina and Hagelia, Per and Persson, Frank and Modin, Oskar and Martins, Paula Dalcin and Wilén, Britt Marie}},
issn = {{2045-2322}},
keywords = {{Amplicon sequencing; Biodeterioration; Biofilm community; Fibre-reinforced sprayed concrete; subsea tunnels; Metagenomics}},
language = {{eng}},
number = {{1}},
publisher = {{Nature Publishing Group}},
series = {{Scientific Reports}},
title = {{Microbial acidification by N, S, Fe and Mn oxidation as a key mechanism for deterioration of subsea tunnel sprayed concrete}},
url = {{http://dx.doi.org/10.1038/s41598-024-73911-w}},
doi = {{10.1038/s41598-024-73911-w}},
volume = {{14}},
year = {{2024}},
}