Plasminogen enhances virulence of group A streptococci by streptokinase-dependent and streptokinase-independent mechanisms
(2003) In Journal of Infectious Diseases 188(4). p.497-505- Abstract
- Interactions between host plasminogen (Plg) and streptokinase (SK) secreted by group A streptococci ( GAS) have been hypothesized to promote bacterial invasion of tissues. The virulence of GAS strain UMAA2616, after being subcutaneously inoculated into mice, was studied. Skin lesions and mortality were observed after inoculation of 7 x 10(6) cfu. Coadministration of human Plg with UMAA2616 markedly increased virulence. SK-deficient UMAA2616 (UMAA2616-SK-) was generated. Mean skin-lesion area and mortality, after bacterial inoculation (3 x 10(5) cfu), were significantly greater with UMAA2616 in the presence of human Plg than with UMAA2616-SK- in the presence of human Plg (P = .0001). Human Plg also enhanced UMAA2616-SK- vir ulence.... (More)
- Interactions between host plasminogen (Plg) and streptokinase (SK) secreted by group A streptococci ( GAS) have been hypothesized to promote bacterial invasion of tissues. The virulence of GAS strain UMAA2616, after being subcutaneously inoculated into mice, was studied. Skin lesions and mortality were observed after inoculation of 7 x 10(6) cfu. Coadministration of human Plg with UMAA2616 markedly increased virulence. SK-deficient UMAA2616 (UMAA2616-SK-) was generated. Mean skin-lesion area and mortality, after bacterial inoculation (3 x 10(5) cfu), were significantly greater with UMAA2616 in the presence of human Plg than with UMAA2616-SK- in the presence of human Plg (P = .0001). Human Plg also enhanced UMAA2616-SK- vir ulence. Exogenous human Plg enhanced the virulence of MGAS166, a human clinical isolate. These findings suggest that SK-Plg interactions are an important determinant of GAS invasiveness in vivo and that both SK and host Plg activators appear to promote virulence of GAS by catalyzing plasmin formation. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/304307
- author
- Khil, J ; Im, M ; Heath, A ; Ringdahl, Ulrika LU ; Mundada, L ; Engleberg, NC and Fay, WP
- organization
- publishing date
- 2003
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Infectious Diseases
- volume
- 188
- issue
- 4
- pages
- 497 - 505
- publisher
- Oxford University Press
- external identifiers
-
- pmid:12898435
- wos:000184567000003
- scopus:0041440157
- ISSN
- 1537-6613
- DOI
- 10.1086/377100
- language
- English
- LU publication?
- yes
- id
- bfb6fe3c-51e1-4ba6-8409-032fcda9eae1 (old id 304307)
- date added to LUP
- 2016-04-01 16:37:10
- date last changed
- 2022-01-28 20:55:57
@article{bfb6fe3c-51e1-4ba6-8409-032fcda9eae1, abstract = {{Interactions between host plasminogen (Plg) and streptokinase (SK) secreted by group A streptococci ( GAS) have been hypothesized to promote bacterial invasion of tissues. The virulence of GAS strain UMAA2616, after being subcutaneously inoculated into mice, was studied. Skin lesions and mortality were observed after inoculation of 7 x 10(6) cfu. Coadministration of human Plg with UMAA2616 markedly increased virulence. SK-deficient UMAA2616 (UMAA2616-SK-) was generated. Mean skin-lesion area and mortality, after bacterial inoculation (3 x 10(5) cfu), were significantly greater with UMAA2616 in the presence of human Plg than with UMAA2616-SK- in the presence of human Plg (P = .0001). Human Plg also enhanced UMAA2616-SK- vir ulence. Exogenous human Plg enhanced the virulence of MGAS166, a human clinical isolate. These findings suggest that SK-Plg interactions are an important determinant of GAS invasiveness in vivo and that both SK and host Plg activators appear to promote virulence of GAS by catalyzing plasmin formation.}}, author = {{Khil, J and Im, M and Heath, A and Ringdahl, Ulrika and Mundada, L and Engleberg, NC and Fay, WP}}, issn = {{1537-6613}}, language = {{eng}}, number = {{4}}, pages = {{497--505}}, publisher = {{Oxford University Press}}, series = {{Journal of Infectious Diseases}}, title = {{Plasminogen enhances virulence of group A streptococci by streptokinase-dependent and streptokinase-independent mechanisms}}, url = {{http://dx.doi.org/10.1086/377100}}, doi = {{10.1086/377100}}, volume = {{188}}, year = {{2003}}, }