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The Bacillus subtilis ctaB paralogue, yjdK, can complement the heme A synthesis deficiency of a CtaB-deficient mutant

Throne-Holst, Mimmi LU and Hederstedt, Lars LU (2000) In FEMS Microbiology Letters 183. p.247-251
Abstract
Heme A is a prosthetic group in many respiratory oxidases. It is synthesised from heme B (protoheme IX) with heme O as an intermediate. In Bacillus subtilis two genes required for heme A synthesis, ctaA and ctaB, have been identified. CtaB is the heme O synthase and CtaA is involved in the conversion of heme O to heme A. A ctaB paralogue, yjdK, has been identified through the B. subtilis genome sequencing project. In this study we show that when carried on a low copy number plasmid, the yjdK gene can complement a ctaB deletion mutant with respect to heme A synthesis. Our results indicate that YjdK has heme O synthase activity. We therefore suggest that yjdK be renamed as ctaO.
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Cytochrome a, Heme 0, Respiratory oxidase
in
FEMS Microbiology Letters
volume
183
pages
247 - 251
publisher
Elsevier
ISSN
1574-6968
DOI
10.1111/j.1574-6968.2000.tb08966.x
language
English
LU publication?
yes
id
c0073e91-91fe-462e-8178-64494fa627ef
date added to LUP
2017-07-17 12:07:23
date last changed
2017-08-11 10:53:07
@article{c0073e91-91fe-462e-8178-64494fa627ef,
  abstract     = {Heme A is a prosthetic group in many respiratory oxidases. It is synthesised from heme B (protoheme IX) with heme O as an intermediate. In Bacillus subtilis two genes required for heme A synthesis, ctaA and ctaB, have been identified. CtaB is the heme O synthase and CtaA is involved in the conversion of heme O to heme A. A ctaB paralogue, yjdK, has been identified through the B. subtilis genome sequencing project. In this study we show that when carried on a low copy number plasmid, the yjdK gene can complement a ctaB deletion mutant with respect to heme A synthesis. Our results indicate that YjdK has heme O synthase activity. We therefore suggest that yjdK be renamed as ctaO.},
  author       = {Throne-Holst, Mimmi and Hederstedt, Lars},
  issn         = {1574-6968},
  keyword      = {Cytochrome a,Heme 0,Respiratory oxidase},
  language     = {eng},
  pages        = {247--251},
  publisher    = {Elsevier},
  series       = {FEMS Microbiology Letters},
  title        = {The <em>Bacillus subtilis ctaB</em> paralogue, <em>yjdK</em>, can complement the heme A synthesis deficiency of a CtaB-deficient mutant},
  url          = {http://dx.doi.org/10.1111/j.1574-6968.2000.tb08966.x},
  volume       = {183},
  year         = {2000},
}