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The Bacillus subtilis ctaB paralogue, yjdK, can complement the heme A synthesis deficiency of a CtaB-deficient mutant

Throne-Holst, Mimmi LU and Hederstedt, Lars LU (2000) In FEMS Microbiology Letters 183. p.247-251
Abstract
Heme A is a prosthetic group in many respiratory oxidases. It is synthesised from heme B (protoheme IX) with heme O as an intermediate. In Bacillus subtilis two genes required for heme A synthesis, ctaA and ctaB, have been identified. CtaB is the heme O synthase and CtaA is involved in the conversion of heme O to heme A. A ctaB paralogue, yjdK, has been identified through the B. subtilis genome sequencing project. In this study we show that when carried on a low copy number plasmid, the yjdK gene can complement a ctaB deletion mutant with respect to heme A synthesis. Our results indicate that YjdK has heme O synthase activity. We therefore suggest that yjdK be renamed as ctaO.
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publishing date
type
Contribution to journal
publication status
published
subject
keywords
Cytochrome a, Heme 0, Respiratory oxidase
in
FEMS Microbiology Letters
volume
183
pages
247 - 251
publisher
Oxford University Press
ISSN
1574-6968
DOI
10.1111/j.1574-6968.2000.tb08966.x
language
English
LU publication?
yes
id
c0073e91-91fe-462e-8178-64494fa627ef
date added to LUP
2017-07-17 12:07:23
date last changed
2025-04-04 13:51:18
@article{c0073e91-91fe-462e-8178-64494fa627ef,
  abstract     = {{Heme A is a prosthetic group in many respiratory oxidases. It is synthesised from heme B (protoheme IX) with heme O as an intermediate. In Bacillus subtilis two genes required for heme A synthesis, ctaA and ctaB, have been identified. CtaB is the heme O synthase and CtaA is involved in the conversion of heme O to heme A. A ctaB paralogue, yjdK, has been identified through the B. subtilis genome sequencing project. In this study we show that when carried on a low copy number plasmid, the yjdK gene can complement a ctaB deletion mutant with respect to heme A synthesis. Our results indicate that YjdK has heme O synthase activity. We therefore suggest that yjdK be renamed as ctaO.}},
  author       = {{Throne-Holst, Mimmi and Hederstedt, Lars}},
  issn         = {{1574-6968}},
  keywords     = {{Cytochrome a; Heme 0; Respiratory oxidase}},
  language     = {{eng}},
  pages        = {{247--251}},
  publisher    = {{Oxford University Press}},
  series       = {{FEMS Microbiology Letters}},
  title        = {{The <em>Bacillus subtilis ctaB</em> paralogue, <em>yjdK</em>, can complement the heme A synthesis deficiency of a CtaB-deficient mutant}},
  url          = {{http://dx.doi.org/10.1111/j.1574-6968.2000.tb08966.x}},
  doi          = {{10.1111/j.1574-6968.2000.tb08966.x}},
  volume       = {{183}},
  year         = {{2000}},
}