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Integration of extraction with affinity precipitation

Kamihira, Masamichi ; Hatti-Kaul, Rajni LU and Mattiasson, Bo LU (2000) p.371-379
Abstract
Conventional aqueous two-phase extraction by spontaneous partitioning has often problems with low specificity. The introduction of an affinity ligand into one of the phases has been attempted to enhance the specificity of protein partitioning (1-7; Chapters 29-31). However, this procedure still has some limitations in the effective removal of impurities as well as recovery and reuse of the ligand. During the past decade, another potentially scalable technique, affinity precipitation, has been studied for the isolation of proteins (8-10). The affinity ligand coupled to a reversibly soluble-insoluble polymer is used to specifically bind the protein in a complex mixture. The precipitation step which can be accomplished by a change in any... (More)
Conventional aqueous two-phase extraction by spontaneous partitioning has often problems with low specificity. The introduction of an affinity ligand into one of the phases has been attempted to enhance the specificity of protein partitioning (1-7; Chapters 29-31). However, this procedure still has some limitations in the effective removal of impurities as well as recovery and reuse of the ligand. During the past decade, another potentially scalable technique, affinity precipitation, has been studied for the isolation of proteins (8-10). The affinity ligand coupled to a reversibly soluble-insoluble polymer is used to specifically bind the protein in a complex mixture. The precipitation step which can be accomplished by a change in any environmental parameter such as pH, temperature, salinity, and so forth, facilitates the separation of the bound ligand and affinity complex from the unbound components. Although cells and cell debris should be removed before use, recovery and reuse of the ligand are relatively easy. (Less)
Please use this url to cite or link to this publication:
author
; and
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
host publication
Aqueous Two-Phase Systems : Methods and Protocols - Methods and Protocols
editor
Hatti-Kaul, Rajni
pages
371 - 379
publisher
Humana Press
ISBN
978-1-59259-028-5
978-0-89603-541-6
978-1-61737-067-0
DOI
10.1385/1-59259-028-4:371
language
English
LU publication?
yes
id
c0a0adf5-985c-4bbe-abad-7dc1db294dc9
date added to LUP
2019-06-30 09:28:33
date last changed
2021-10-22 12:54:46
@inbook{c0a0adf5-985c-4bbe-abad-7dc1db294dc9,
  abstract     = {Conventional aqueous two-phase extraction by spontaneous partitioning has often problems with low specificity. The introduction of an affinity ligand into one of the phases has been attempted to enhance the specificity of protein partitioning (1-7; Chapters 29-31). However, this procedure still has some limitations in the effective removal of impurities as well as recovery and reuse of the ligand. During the past decade, another potentially scalable technique, affinity precipitation, has been studied for the isolation of proteins (8-10). The affinity ligand coupled to a reversibly soluble-insoluble polymer is used to specifically bind the protein in a complex mixture. The precipitation step which can be accomplished by a change in any environmental parameter such as pH, temperature, salinity, and so forth, facilitates the separation of the bound ligand and affinity complex from the unbound components. Although cells and cell debris should be removed before use, recovery and reuse of the ligand are relatively easy.},
  author       = {Kamihira, Masamichi and Hatti-Kaul, Rajni and Mattiasson, Bo},
  booktitle    = {Aqueous Two-Phase Systems : Methods and Protocols},
  editor       = {Hatti-Kaul, Rajni},
  isbn         = {978-1-59259-028-5},
  language     = {eng},
  pages        = {371--379},
  publisher    = {Humana Press},
  title        = {Integration of extraction with affinity precipitation},
  url          = {http://dx.doi.org/10.1385/1-59259-028-4:371},
  doi          = {10.1385/1-59259-028-4:371},
  year         = {2000},
}