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Receptor for alpha1-microglobulin on T lymphocytes : inhibition of antigen-induced interleukin-2 production

Wester, L ; Michaëlsson, E LU ; Holmdahl, R LU ; Olofsson, T LU and Åkerström, B LU (1998) In Scandinavian Journal of Immunology 48(1). p.1-7
Abstract

The human plasma protein alpha1-microglobulin (alpha1m) was found to inhibit the antigen-induced interleukin-2 (IL-2) production of two different mouse T-helper cell hybridomas. Alpha1m isolated from human plasma and recombinant alpha1m isolated from baculovirus-infected insect cell cultures had similar inhibitory effects. Flow cytometric analysis showed a binding of plasma and recombinant alpha1m to the T-cell hybridomas as well as to a human T-cell line. Radiolabelled plasma and recombinant alpha1m bound to the T-cell hybridomas in a saturable manner and the binding could be eliminated by trypsination of the cells. The affinity constants for the cell binding were calculated to be 0.4-1 x 10(5) M(-1) using Scatchard plotting, and the... (More)

The human plasma protein alpha1-microglobulin (alpha1m) was found to inhibit the antigen-induced interleukin-2 (IL-2) production of two different mouse T-helper cell hybridomas. Alpha1m isolated from human plasma and recombinant alpha1m isolated from baculovirus-infected insect cell cultures had similar inhibitory effects. Flow cytometric analysis showed a binding of plasma and recombinant alpha1m to the T-cell hybridomas as well as to a human T-cell line. Radiolabelled plasma and recombinant alpha1m bound to the T-cell hybridomas in a saturable manner and the binding could be eliminated by trypsination of the cells. The affinity constants for the cell binding were calculated to be 0.4-1 x 10(5) M(-1) using Scatchard plotting, and the number of binding sites per cell was estimated to be 5 x 10(5)-1 x 10(6). The cell-surface proteins of one of the T-cell hybridomas were radiolabelled, the cells lysed and alpha1m-binding proteins isolated by affinity chromatography. SDS-PAGE and autoradiography analysis of the eluate revealed major bands with Mr-values around 70, 35 and 15 kDa. The results thus suggest that alpha1m binds to a specific receptor on T cells and that the binding leads to inhibition of antigen-stimulated IL-2 production by T-helper cells.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Alpha-Globulins/metabolism, Animals, Antigens/immunology, Collagen/immunology, Humans, Interleukin-2/biosynthesis, Mice, Receptors, Immunologic/metabolism, T-Lymphocytes/metabolism, Tumor Cells, Cultured
in
Scandinavian Journal of Immunology
volume
48
issue
1
pages
7 pages
publisher
Wiley-Blackwell
external identifiers
  • pmid:9714404
  • scopus:0031594846
ISSN
0300-9475
DOI
10.1046/j.1365-3083.1998.00378.x
language
English
LU publication?
yes
id
c0cce44d-a177-4031-beeb-84674ea8f6bb
date added to LUP
2019-05-22 10:13:28
date last changed
2020-01-13 01:51:49
@article{c0cce44d-a177-4031-beeb-84674ea8f6bb,
  abstract     = {<p>The human plasma protein alpha1-microglobulin (alpha1m) was found to inhibit the antigen-induced interleukin-2 (IL-2) production of two different mouse T-helper cell hybridomas. Alpha1m isolated from human plasma and recombinant alpha1m isolated from baculovirus-infected insect cell cultures had similar inhibitory effects. Flow cytometric analysis showed a binding of plasma and recombinant alpha1m to the T-cell hybridomas as well as to a human T-cell line. Radiolabelled plasma and recombinant alpha1m bound to the T-cell hybridomas in a saturable manner and the binding could be eliminated by trypsination of the cells. The affinity constants for the cell binding were calculated to be 0.4-1 x 10(5) M(-1) using Scatchard plotting, and the number of binding sites per cell was estimated to be 5 x 10(5)-1 x 10(6). The cell-surface proteins of one of the T-cell hybridomas were radiolabelled, the cells lysed and alpha1m-binding proteins isolated by affinity chromatography. SDS-PAGE and autoradiography analysis of the eluate revealed major bands with Mr-values around 70, 35 and 15 kDa. The results thus suggest that alpha1m binds to a specific receptor on T cells and that the binding leads to inhibition of antigen-stimulated IL-2 production by T-helper cells.</p>},
  author       = {Wester, L and Michaëlsson, E and Holmdahl, R and Olofsson, T and Åkerström, B},
  issn         = {0300-9475},
  language     = {eng},
  number       = {1},
  pages        = {1--7},
  publisher    = {Wiley-Blackwell},
  series       = {Scandinavian Journal of Immunology},
  title        = {Receptor for alpha1-microglobulin on T lymphocytes : inhibition of antigen-induced interleukin-2 production},
  url          = {http://dx.doi.org/10.1046/j.1365-3083.1998.00378.x},
  doi          = {10.1046/j.1365-3083.1998.00378.x},
  volume       = {48},
  year         = {1998},
}