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Interleukin-25 reduces Th17 cells and inflammatory responses in human peripheral blood mononuclear cells

Mantani, Polyxeni T. LU ; Vallejo, Jenifer LU ; Ljungcrantz, Irena LU ; Nilsson, Jan LU ; Björkbacka, Harry LU and Fredrikson, Gunilla Nordin LU (2018) In Human Immunology 79(9). p.685-692
Abstract

Background: The absence of interleukin-25 (IL-25) favors the induction of Th1 and Th17 immune responses in mice. Th1 immune responses have been associated with the pathology of atherosclerosis, a lipid and inflammation driven disease of the arterial wall. Purpose of research: To evaluate the effect of IL-25 on human peripheral blood mononuclear cells (hPBMCs) in the presence and absence of oxidized low density lipoprotein (oxLDL), a key player in atherosclerosis development. Principal results: Human PBMCs were incubated with recombinant human IL-25 (rhIL-25) in the presence and absence of oxLDL and analyzed with flow cytometry while cytokine secretion was measured in cell culture supernatants. The IL-25 receptor, IL-17RB, was mostly... (More)

Background: The absence of interleukin-25 (IL-25) favors the induction of Th1 and Th17 immune responses in mice. Th1 immune responses have been associated with the pathology of atherosclerosis, a lipid and inflammation driven disease of the arterial wall. Purpose of research: To evaluate the effect of IL-25 on human peripheral blood mononuclear cells (hPBMCs) in the presence and absence of oxidized low density lipoprotein (oxLDL), a key player in atherosclerosis development. Principal results: Human PBMCs were incubated with recombinant human IL-25 (rhIL-25) in the presence and absence of oxLDL and analyzed with flow cytometry while cytokine secretion was measured in cell culture supernatants. The IL-25 receptor, IL-17RB, was mostly expressed on T cells. Incubation of hPBMCs with IL-25 reduced the frequency of Th17 cells. Furthermore, IL-25 inhibited the release of the Th17-inducing cytokine IL-6 from dendritic cells isolated from hPBMCs indicating that the IL-25 mediated Th17 suppression may be indirect. Moreover, IL-25 reduced the secretion of the proinflammatory cytokine IFNγ from hPBMCs. OxLDL decreased IFNγ release from hPBMCs regardless of the presence or absence of IL-25. Conclusions: IL-25 reduces Th1 and Th17 immune responses in hPBMCs raising the interesting possibility that IL-25 could have a protective role in human atherosclerosis.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
IL-25, Oxidized LDL, Th17
in
Human Immunology
volume
79
issue
9
pages
685 - 692
publisher
Elsevier
external identifiers
  • scopus:85049309749
ISSN
0198-8859
DOI
10.1016/j.humimm.2018.06.008
language
English
LU publication?
yes
id
c143869b-d229-4a7c-9296-50b0d138bcac
date added to LUP
2018-07-17 12:06:08
date last changed
2019-01-14 16:49:16
@article{c143869b-d229-4a7c-9296-50b0d138bcac,
  abstract     = {<p>Background: The absence of interleukin-25 (IL-25) favors the induction of Th1 and Th17 immune responses in mice. Th1 immune responses have been associated with the pathology of atherosclerosis, a lipid and inflammation driven disease of the arterial wall. Purpose of research: To evaluate the effect of IL-25 on human peripheral blood mononuclear cells (hPBMCs) in the presence and absence of oxidized low density lipoprotein (oxLDL), a key player in atherosclerosis development. Principal results: Human PBMCs were incubated with recombinant human IL-25 (rhIL-25) in the presence and absence of oxLDL and analyzed with flow cytometry while cytokine secretion was measured in cell culture supernatants. The IL-25 receptor, IL-17RB, was mostly expressed on T cells. Incubation of hPBMCs with IL-25 reduced the frequency of Th17 cells. Furthermore, IL-25 inhibited the release of the Th17-inducing cytokine IL-6 from dendritic cells isolated from hPBMCs indicating that the IL-25 mediated Th17 suppression may be indirect. Moreover, IL-25 reduced the secretion of the proinflammatory cytokine IFNγ from hPBMCs. OxLDL decreased IFNγ release from hPBMCs regardless of the presence or absence of IL-25. Conclusions: IL-25 reduces Th1 and Th17 immune responses in hPBMCs raising the interesting possibility that IL-25 could have a protective role in human atherosclerosis.</p>},
  author       = {Mantani, Polyxeni T. and Vallejo, Jenifer and Ljungcrantz, Irena and Nilsson, Jan and Björkbacka, Harry and Fredrikson, Gunilla Nordin},
  issn         = {0198-8859},
  keyword      = {IL-25,Oxidized LDL,Th17},
  language     = {eng},
  month        = {01},
  number       = {9},
  pages        = {685--692},
  publisher    = {Elsevier},
  series       = {Human Immunology},
  title        = {Interleukin-25 reduces Th17 cells and inflammatory responses in human peripheral blood mononuclear cells},
  url          = {http://dx.doi.org/10.1016/j.humimm.2018.06.008},
  volume       = {79},
  year         = {2018},
}