Interleukin-25 reduces Th17 cells and inflammatory responses in human peripheral blood mononuclear cells
(2018) In Human Immunology 79(9). p.685-692- Abstract
Background: The absence of interleukin-25 (IL-25) favors the induction of Th1 and Th17 immune responses in mice. Th1 immune responses have been associated with the pathology of atherosclerosis, a lipid and inflammation driven disease of the arterial wall. Purpose of research: To evaluate the effect of IL-25 on human peripheral blood mononuclear cells (hPBMCs) in the presence and absence of oxidized low density lipoprotein (oxLDL), a key player in atherosclerosis development. Principal results: Human PBMCs were incubated with recombinant human IL-25 (rhIL-25) in the presence and absence of oxLDL and analyzed with flow cytometry while cytokine secretion was measured in cell culture supernatants. The IL-25 receptor, IL-17RB, was mostly... (More)
Background: The absence of interleukin-25 (IL-25) favors the induction of Th1 and Th17 immune responses in mice. Th1 immune responses have been associated with the pathology of atherosclerosis, a lipid and inflammation driven disease of the arterial wall. Purpose of research: To evaluate the effect of IL-25 on human peripheral blood mononuclear cells (hPBMCs) in the presence and absence of oxidized low density lipoprotein (oxLDL), a key player in atherosclerosis development. Principal results: Human PBMCs were incubated with recombinant human IL-25 (rhIL-25) in the presence and absence of oxLDL and analyzed with flow cytometry while cytokine secretion was measured in cell culture supernatants. The IL-25 receptor, IL-17RB, was mostly expressed on T cells. Incubation of hPBMCs with IL-25 reduced the frequency of Th17 cells. Furthermore, IL-25 inhibited the release of the Th17-inducing cytokine IL-6 from dendritic cells isolated from hPBMCs indicating that the IL-25 mediated Th17 suppression may be indirect. Moreover, IL-25 reduced the secretion of the proinflammatory cytokine IFNγ from hPBMCs. OxLDL decreased IFNγ release from hPBMCs regardless of the presence or absence of IL-25. Conclusions: IL-25 reduces Th1 and Th17 immune responses in hPBMCs raising the interesting possibility that IL-25 could have a protective role in human atherosclerosis.
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- author
- Mantani, Polyxeni T. LU ; Vallejo, Jenifer LU ; Ljungcrantz, Irena LU ; Nilsson, Jan LU ; Björkbacka, Harry LU and Fredrikson, Gunilla Nordin LU
- organization
- publishing date
- 2018-01-01
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- IL-25, Oxidized LDL, Th17
- in
- Human Immunology
- volume
- 79
- issue
- 9
- pages
- 685 - 692
- publisher
- Elsevier
- external identifiers
-
- pmid:29966691
- scopus:85049309749
- ISSN
- 0198-8859
- DOI
- 10.1016/j.humimm.2018.06.008
- language
- English
- LU publication?
- yes
- id
- c143869b-d229-4a7c-9296-50b0d138bcac
- date added to LUP
- 2018-07-17 12:06:08
- date last changed
- 2024-08-19 20:37:18
@article{c143869b-d229-4a7c-9296-50b0d138bcac, abstract = {{<p>Background: The absence of interleukin-25 (IL-25) favors the induction of Th1 and Th17 immune responses in mice. Th1 immune responses have been associated with the pathology of atherosclerosis, a lipid and inflammation driven disease of the arterial wall. Purpose of research: To evaluate the effect of IL-25 on human peripheral blood mononuclear cells (hPBMCs) in the presence and absence of oxidized low density lipoprotein (oxLDL), a key player in atherosclerosis development. Principal results: Human PBMCs were incubated with recombinant human IL-25 (rhIL-25) in the presence and absence of oxLDL and analyzed with flow cytometry while cytokine secretion was measured in cell culture supernatants. The IL-25 receptor, IL-17RB, was mostly expressed on T cells. Incubation of hPBMCs with IL-25 reduced the frequency of Th17 cells. Furthermore, IL-25 inhibited the release of the Th17-inducing cytokine IL-6 from dendritic cells isolated from hPBMCs indicating that the IL-25 mediated Th17 suppression may be indirect. Moreover, IL-25 reduced the secretion of the proinflammatory cytokine IFNγ from hPBMCs. OxLDL decreased IFNγ release from hPBMCs regardless of the presence or absence of IL-25. Conclusions: IL-25 reduces Th1 and Th17 immune responses in hPBMCs raising the interesting possibility that IL-25 could have a protective role in human atherosclerosis.</p>}}, author = {{Mantani, Polyxeni T. and Vallejo, Jenifer and Ljungcrantz, Irena and Nilsson, Jan and Björkbacka, Harry and Fredrikson, Gunilla Nordin}}, issn = {{0198-8859}}, keywords = {{IL-25; Oxidized LDL; Th17}}, language = {{eng}}, month = {{01}}, number = {{9}}, pages = {{685--692}}, publisher = {{Elsevier}}, series = {{Human Immunology}}, title = {{Interleukin-25 reduces Th17 cells and inflammatory responses in human peripheral blood mononuclear cells}}, url = {{http://dx.doi.org/10.1016/j.humimm.2018.06.008}}, doi = {{10.1016/j.humimm.2018.06.008}}, volume = {{79}}, year = {{2018}}, }