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Delineating closely related dinoflagellate lineages using phylotranscriptomics

Annenkova, Nataliia V. LU ; Ahrén, Dag LU ; Logares, Ramiro LU ; Kremp, Anke and Rengefors, Karin LU (2018) In Journal of Phycology 54(4). p.571-576
Abstract

Recently radiated dinoflagellates Apocalathium aciculiferum (collected in Lake Erken, Sweden), Apocalathium malmogiense (Baltic Sea) and Apocalathium aff. malmogiense (Highway Lake, Antarctica) represent a lineage with an unresolved phylogeny. We determined their phylogenetic relationships using phylotranscriptomics based on 792 amino acid sequences. Our results showed that A. aciculiferum diverged from the other two closely related lineages, consistent with their different morphologies in cell size, relative cell length and presence of spines. We hypothesized that A. aff. malmogiense and A. malmogiense, which inhabit different hemispheres, are evolutionarily more closely related because they diverged from a marine common ancestor,... (More)

Recently radiated dinoflagellates Apocalathium aciculiferum (collected in Lake Erken, Sweden), Apocalathium malmogiense (Baltic Sea) and Apocalathium aff. malmogiense (Highway Lake, Antarctica) represent a lineage with an unresolved phylogeny. We determined their phylogenetic relationships using phylotranscriptomics based on 792 amino acid sequences. Our results showed that A. aciculiferum diverged from the other two closely related lineages, consistent with their different morphologies in cell size, relative cell length and presence of spines. We hypothesized that A. aff. malmogiense and A. malmogiense, which inhabit different hemispheres, are evolutionarily more closely related because they diverged from a marine common ancestor, adapting to a wide salinity range, while A. aciculiferum colonized a freshwater habitat, by acquiring adaptations to this environment, in particular, salinity intolerance. We show that phylotranscriptomics can resolve the phylogeny of recently diverged protists. This has broad relevance, given that many phytoplankton species are morphologically very similar, and single genes sometimes lack the information to determine species’ relationships.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
adaptive radiation, High Throughput Sequencing, microalgae, phylogenomics, transcriptome, protists
in
Journal of Phycology
volume
54
issue
4
pages
6 pages
publisher
Wiley-Blackwell
external identifiers
  • scopus:85051419777
ISSN
0022-3646
DOI
10.1111/jpy.12748
language
English
LU publication?
yes
id
c18a92f6-e2be-4cfa-88d9-a7a6bef27ccb
date added to LUP
2018-09-10 14:42:05
date last changed
2019-02-20 11:26:15
@misc{c18a92f6-e2be-4cfa-88d9-a7a6bef27ccb,
  abstract     = {<p>Recently radiated dinoflagellates Apocalathium aciculiferum (collected in Lake Erken, Sweden), Apocalathium malmogiense (Baltic Sea) and Apocalathium aff. malmogiense (Highway Lake, Antarctica) represent a lineage with an unresolved phylogeny. We determined their phylogenetic relationships using phylotranscriptomics based on 792 amino acid sequences. Our results showed that A. aciculiferum diverged from the other two closely related lineages, consistent with their different morphologies in cell size, relative cell length and presence of spines. We hypothesized that A. aff. malmogiense and A. malmogiense, which inhabit different hemispheres, are evolutionarily more closely related because they diverged from a marine common ancestor, adapting to a wide salinity range, while A. aciculiferum colonized a freshwater habitat, by acquiring adaptations to this environment, in particular, salinity intolerance. We show that phylotranscriptomics can resolve the phylogeny of recently diverged protists. This has broad relevance, given that many phytoplankton species are morphologically very similar, and single genes sometimes lack the information to determine species’ relationships.</p>},
  author       = {Annenkova, Nataliia V. and Ahrén, Dag and Logares, Ramiro and Kremp, Anke and Rengefors, Karin},
  issn         = {0022-3646},
  keyword      = {adaptive radiation,High Throughput Sequencing,microalgae,phylogenomics, transcriptome,protists},
  language     = {eng},
  month        = {08},
  number       = {4},
  pages        = {571--576},
  publisher    = {Wiley-Blackwell},
  series       = {Journal of Phycology},
  title        = {Delineating closely related dinoflagellate lineages using phylotranscriptomics},
  url          = {http://dx.doi.org/10.1111/jpy.12748},
  volume       = {54},
  year         = {2018},
}