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Tuning biomimetic membrane barrier properties by hydrocarbon, cholesterol and polymeric additives

Palanco, Marta Espina LU ; Skovgaard, Nils; Hansen, Jesper Sondergaard LU ; Berg-Sorensen, Kirstine and Hélix-Nielsen, Claus (2018) In Bioinspiration and Biomimetics 13(1).
Abstract

The barrier properties of cellular membranes are increasingly attracting attention as a source of inspiration for designing biomimetic membranes. The broad range of potential technological applications makes the use of lipid and lately also polymeric materials a popular choice for constructing biomimetic membranes, where the barrier properties can be controlled by the composition of the membrane constituent elements. Here we investigate the membrane properties reported by the light-induced proton pumping activity of bacteriorhodopsin (bR) reconstituted in three vesicle systems of different membrane composition. Specifically we quantify how the resulting proton influx and efflux rates are influenced by the membrane composition using a... (More)

The barrier properties of cellular membranes are increasingly attracting attention as a source of inspiration for designing biomimetic membranes. The broad range of potential technological applications makes the use of lipid and lately also polymeric materials a popular choice for constructing biomimetic membranes, where the barrier properties can be controlled by the composition of the membrane constituent elements. Here we investigate the membrane properties reported by the light-induced proton pumping activity of bacteriorhodopsin (bR) reconstituted in three vesicle systems of different membrane composition. Specifically we quantify how the resulting proton influx and efflux rates are influenced by the membrane composition using a variety of membrane modulators. We demonstrate that by adding hydrocarbons to vesicles with reconstituted bR formed from asolectin lipids the resulting transmembrane proton fluxes changes proportional to the carbon chain length when compared against control. We observe a similar proportionality in single-component 1,2-Dioleoyl-sn-glycero-3-phosphocholine model membranes when using cholesterol. Lastly we investigate the effects of adding the amphiphilic di-block co-polymer polybutadiene-polyethyleneoxide (PB12-PEO10) to phospholipid membranes formed from 1,2-Dioleoyl-sn-glycero-3-phosphocholine, 1,2-Dioleoyl-sn-glycero-3-phosphatidylethanolamine, and 1,2-Dioleoyl-sn-glycero-3-phosphatidylserine. The proton pumping activity of bR (measured as a change in extra-vesicular pH) in mixed lipid/PB12-PEO10 lipid systems is up to six-fold higher compared to that observed for bR containing vesicles made from PB12-PEO10 alone. Interestingly, bR inserts with apparent opposite orientation in pure PB12-PEO10 vesicles as compared to pure lipid vesicles. Addition of equimolar amounts of lipids to PB12-PEO10 results in bR orientation similar to that observed for pure lipids. In conclusion our results show how the barrier properties of the membranes can be controlled by the composition of the membrane. In particular the use of mixed lipid-polymer systems may pave the way for constructing biomimetic membranes tailored for optimal properties in various applications including drug delivery systems, biosensors and energy conservation technology.

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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
asolectin, bacteriorhodopsin, biomimetic membranes, cholesterol, diblock copolymer, lipid membrane, phospholipids
in
Bioinspiration and Biomimetics
volume
13
issue
1
publisher
IOP Publishing
external identifiers
  • scopus:85047488083
ISSN
1748-3182
DOI
10.1088/1748-3190/aa92be
language
English
LU publication?
yes
id
c1bdd301-909c-4ff4-ba93-48070040e6c7
date added to LUP
2018-06-07 10:49:40
date last changed
2019-03-19 03:55:48
@article{c1bdd301-909c-4ff4-ba93-48070040e6c7,
  abstract     = {<p>The barrier properties of cellular membranes are increasingly attracting attention as a source of inspiration for designing biomimetic membranes. The broad range of potential technological applications makes the use of lipid and lately also polymeric materials a popular choice for constructing biomimetic membranes, where the barrier properties can be controlled by the composition of the membrane constituent elements. Here we investigate the membrane properties reported by the light-induced proton pumping activity of bacteriorhodopsin (bR) reconstituted in three vesicle systems of different membrane composition. Specifically we quantify how the resulting proton influx and efflux rates are influenced by the membrane composition using a variety of membrane modulators. We demonstrate that by adding hydrocarbons to vesicles with reconstituted bR formed from asolectin lipids the resulting transmembrane proton fluxes changes proportional to the carbon chain length when compared against control. We observe a similar proportionality in single-component 1,2-Dioleoyl-sn-glycero-3-phosphocholine model membranes when using cholesterol. Lastly we investigate the effects of adding the amphiphilic di-block co-polymer polybutadiene-polyethyleneoxide (PB<sub>12</sub>-PEO<sub>10</sub>) to phospholipid membranes formed from 1,2-Dioleoyl-sn-glycero-3-phosphocholine, 1,2-Dioleoyl-sn-glycero-3-phosphatidylethanolamine, and 1,2-Dioleoyl-sn-glycero-3-phosphatidylserine. The proton pumping activity of bR (measured as a change in extra-vesicular pH) in mixed lipid/PB<sub>12</sub>-PEO<sub>10</sub> lipid systems is up to six-fold higher compared to that observed for bR containing vesicles made from PB<sub>12</sub>-PEO<sub>10</sub> alone. Interestingly, bR inserts with apparent opposite orientation in pure PB<sub>12</sub>-PEO<sub>10</sub> vesicles as compared to pure lipid vesicles. Addition of equimolar amounts of lipids to PB<sub>12</sub>-PEO<sub>10</sub> results in bR orientation similar to that observed for pure lipids. In conclusion our results show how the barrier properties of the membranes can be controlled by the composition of the membrane. In particular the use of mixed lipid-polymer systems may pave the way for constructing biomimetic membranes tailored for optimal properties in various applications including drug delivery systems, biosensors and energy conservation technology.</p>},
  articleno    = {016005},
  author       = {Palanco, Marta Espina and Skovgaard, Nils and Hansen, Jesper Sondergaard and Berg-Sorensen, Kirstine and Hélix-Nielsen, Claus},
  issn         = {1748-3182},
  keyword      = {asolectin,bacteriorhodopsin,biomimetic membranes,cholesterol,diblock copolymer,lipid membrane,phospholipids},
  language     = {eng},
  month        = {01},
  number       = {1},
  publisher    = {IOP Publishing},
  series       = {Bioinspiration and Biomimetics},
  title        = {Tuning biomimetic membrane barrier properties by hydrocarbon, cholesterol and polymeric additives},
  url          = {http://dx.doi.org/10.1088/1748-3190/aa92be},
  volume       = {13},
  year         = {2018},
}