Fluorescence imaging of frit effects in capillary separations
(2000) In Electrophoresis 21(15). p.3102-3108- Abstract
- The gradient of five dansylated amino acids in a capillary-based separation system commonly used in capillary liquid chromatography (LC) or capillary electrochromatography (CEC) was followed and examined in detail using a detection method based on laser-induced fluorescence imaging. The detection system consisted of an XeCl excimer laser and an image-intensified charge-coupled device (CCD) camera. Fluorescence intensity profiles along the capillary column were displayed and continuously updated on a computer screen. The detector system enabled the separation dynamics in the column to be monitored. The experiments were focused on the course of events, especially at interfaces. The processes occurring at the beginning of the reversed-phase... (More)
- The gradient of five dansylated amino acids in a capillary-based separation system commonly used in capillary liquid chromatography (LC) or capillary electrochromatography (CEC) was followed and examined in detail using a detection method based on laser-induced fluorescence imaging. The detection system consisted of an XeCl excimer laser and an image-intensified charge-coupled device (CCD) camera. Fluorescence intensity profiles along the capillary column were displayed and continuously updated on a computer screen. The detector system enabled the separation dynamics in the column to be monitored. The experiments were focused on the course of events, especially at interfaces. The processes occurring at the beginning of the reversed-phase packing material were studied as well as at the transition from stationary phase to the outlet frit and the open tubular area. Striking differences in signal intensity and separation efficiency were revealed depending on where on the column detection was performed. Furthermore, adsorption of the analytes on the frits was observed. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/2257426
- author
- Behnke, B ; Johansson, Jonas LU ; Bayer, E and Nilsson, S
- organization
- publishing date
- 2000
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Electrophoresis
- volume
- 21
- issue
- 15
- pages
- 3102 - 3108
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- scopus:0033804520
- ISSN
- 0173-0835
- DOI
- 10.1002/1522-2683(20000901)
- language
- English
- LU publication?
- yes
- id
- c227da87-05be-4000-ba6f-13e9163b772c (old id 2257426)
- date added to LUP
- 2016-04-04 08:55:25
- date last changed
- 2022-04-15 21:18:10
@article{c227da87-05be-4000-ba6f-13e9163b772c,
abstract = {{The gradient of five dansylated amino acids in a capillary-based separation system commonly used in capillary liquid chromatography (LC) or capillary electrochromatography (CEC) was followed and examined in detail using a detection method based on laser-induced fluorescence imaging. The detection system consisted of an XeCl excimer laser and an image-intensified charge-coupled device (CCD) camera. Fluorescence intensity profiles along the capillary column were displayed and continuously updated on a computer screen. The detector system enabled the separation dynamics in the column to be monitored. The experiments were focused on the course of events, especially at interfaces. The processes occurring at the beginning of the reversed-phase packing material were studied as well as at the transition from stationary phase to the outlet frit and the open tubular area. Striking differences in signal intensity and separation efficiency were revealed depending on where on the column detection was performed. Furthermore, adsorption of the analytes on the frits was observed.}},
author = {{Behnke, B and Johansson, Jonas and Bayer, E and Nilsson, S}},
issn = {{0173-0835}},
language = {{eng}},
number = {{15}},
pages = {{3102--3108}},
publisher = {{John Wiley & Sons Inc.}},
series = {{Electrophoresis}},
title = {{Fluorescence imaging of frit effects in capillary separations}},
url = {{https://lup.lub.lu.se/search/files/5210732/2297029.pdf}},
doi = {{10.1002/1522-2683(20000901)}},
volume = {{21}},
year = {{2000}},
}