Identification of the Drosophila and Tribolium receptors for the recently discovered insect RYamide neuropeptides
Collin, Caitlin ; Hauser, Frank ; Krogh-Meyer, Peter ; Hansen, Karina K ; Gonzalez de Valdivia, Ernesto LU
; Williamson, Michael
and Grimmelikhuijzen, Cornelis J P
(2011)
In Biochemical and Biophysical Research Communications
412(4).
p.83-578
- Abstract
One year ago, we discovered a new family of insect RYamide neuropeptides, which has the C-terminal consensus sequence FFXXXRYamide, and which is widely occurring in most insects, including the fruitfly Drosophila melanogaster and the red flour beetle Tribolium castaneum (F. Hauser et al., J. Proteome Res. 9 (2010) 5296-5310). Here, we identify a Drosophila G-protein-coupled receptor (GPCR) coded for by gene CG5811 and its Tribolium GPCR ortholog as insect RYamide receptors. The Drosophila RYamide receptor is equally well activated (EC(50), 1×10(-9)M) by the two Drosophila RYamide neuropeptides: RYamide-1 (PVFFVASRYamide) and RYamide-2 (NEHFFLGSRYamide), both contained in a preprohormone coded for by gene CG40733. The Tribolium receptor... (More)
One year ago, we discovered a new family of insect RYamide neuropeptides, which has the C-terminal consensus sequence FFXXXRYamide, and which is widely occurring in most insects, including the fruitfly Drosophila melanogaster and the red flour beetle Tribolium castaneum (F. Hauser et al., J. Proteome Res. 9 (2010) 5296-5310). Here, we identify a Drosophila G-protein-coupled receptor (GPCR) coded for by gene CG5811 and its Tribolium GPCR ortholog as insect RYamide receptors. The Drosophila RYamide receptor is equally well activated (EC(50), 1×10(-9)M) by the two Drosophila RYamide neuropeptides: RYamide-1 (PVFFVASRYamide) and RYamide-2 (NEHFFLGSRYamide), both contained in a preprohormone coded for by gene CG40733. The Tribolium receptor shows a somewhat higher affinity to Tribolium RYamide-2 (ADAFFLGPRYamide; EC(50), 5×10(-9)M) than to Tribolium RYamide-1 (VQNLATFKTMMRYamide; EC(50), 7×10(-8)M), which might be due to the fact that the last peptide does not completely follow the RYamide consensus sequence rule. There are other neuropeptides in insects that have similar C-terminal sequences (RWamide or RFamide), such as the FMRFamides, sulfakinins, myosuppressins, neuropeptides F, and the various short neuropeptides F. Amazingly, these neuropeptides show no cross-reactivity to the Tribolium RYamide receptor, while the Drosophila RYamide receptor is only very slightly activated by high concentrations (>10(-6)M) of neuropeptide F and short neuropeptide F-1, showing that the two RYamide receptors are quite specific for activation by insect RYamides, and that the sequence FFXXXRYamide is needed for effective insect RYamide receptor activation. Phylogenetic tree analyses and other amino acid sequence comparisons show that the insect RYamide receptors are not closely related to any other known insect or invertebrate/vertebrate receptors, including mammalian neuropeptide Y and insect neuropeptide F and short neuropeptide F receptors. Gene expression data published in Flybase (www.flybase.org) show that the Drosophila CG5811 gene is significantly expressed in the hindgut of adult flies, suggesting a role of insect RYamides in digestion or water reabsorption.
(Less)
- author
- Collin, Caitlin
; Hauser, Frank
; Krogh-Meyer, Peter
; Hansen, Karina K
; Gonzalez de Valdivia, Ernesto
LU
; Williamson, Michael
and Grimmelikhuijzen, Cornelis J P
- publishing date
- 2011-09-09
- type
- Contribution to journal
- publication status
- published
- keywords
- Amides, Amino Acid Sequence, Animals, Drosophila Proteins, Drosophila melanogaster, Gene Expression, Molecular Sequence Data, Neuropeptide Y, Phylogeny, Receptors, Neuropeptide Y, Tissue Distribution, Tribolium, Journal Article, Research Support, Non-U.S. Gov't
- in
- Biochemical and Biophysical Research Communications
- volume
- 412
- issue
- 4
- pages
- 6 pages
- publisher
- Elsevier
- external identifiers
-
- pmid:21843505
- scopus:80052634142
- ISSN
- 1090-2104
- DOI
- 10.1016/j.bbrc.2011.07.131
- language
- English
- LU publication?
- no
- id
- c22ab0ec-2b29-460b-8f2a-2a7c04774195
- date added to LUP
- 2018-01-12 17:03:09
- date last changed
- 2024-03-31 23:06:36
@article{c22ab0ec-2b29-460b-8f2a-2a7c04774195,
abstract = {{<p>One year ago, we discovered a new family of insect RYamide neuropeptides, which has the C-terminal consensus sequence FFXXXRYamide, and which is widely occurring in most insects, including the fruitfly Drosophila melanogaster and the red flour beetle Tribolium castaneum (F. Hauser et al., J. Proteome Res. 9 (2010) 5296-5310). Here, we identify a Drosophila G-protein-coupled receptor (GPCR) coded for by gene CG5811 and its Tribolium GPCR ortholog as insect RYamide receptors. The Drosophila RYamide receptor is equally well activated (EC(50), 1×10(-9)M) by the two Drosophila RYamide neuropeptides: RYamide-1 (PVFFVASRYamide) and RYamide-2 (NEHFFLGSRYamide), both contained in a preprohormone coded for by gene CG40733. The Tribolium receptor shows a somewhat higher affinity to Tribolium RYamide-2 (ADAFFLGPRYamide; EC(50), 5×10(-9)M) than to Tribolium RYamide-1 (VQNLATFKTMMRYamide; EC(50), 7×10(-8)M), which might be due to the fact that the last peptide does not completely follow the RYamide consensus sequence rule. There are other neuropeptides in insects that have similar C-terminal sequences (RWamide or RFamide), such as the FMRFamides, sulfakinins, myosuppressins, neuropeptides F, and the various short neuropeptides F. Amazingly, these neuropeptides show no cross-reactivity to the Tribolium RYamide receptor, while the Drosophila RYamide receptor is only very slightly activated by high concentrations (>10(-6)M) of neuropeptide F and short neuropeptide F-1, showing that the two RYamide receptors are quite specific for activation by insect RYamides, and that the sequence FFXXXRYamide is needed for effective insect RYamide receptor activation. Phylogenetic tree analyses and other amino acid sequence comparisons show that the insect RYamide receptors are not closely related to any other known insect or invertebrate/vertebrate receptors, including mammalian neuropeptide Y and insect neuropeptide F and short neuropeptide F receptors. Gene expression data published in Flybase (www.flybase.org) show that the Drosophila CG5811 gene is significantly expressed in the hindgut of adult flies, suggesting a role of insect RYamides in digestion or water reabsorption.</p>}},
author = {{Collin, Caitlin and Hauser, Frank and Krogh-Meyer, Peter and Hansen, Karina K and Gonzalez de Valdivia, Ernesto and Williamson, Michael and Grimmelikhuijzen, Cornelis J P}},
issn = {{1090-2104}},
keywords = {{Amides; Amino Acid Sequence; Animals; Drosophila Proteins; Drosophila melanogaster; Gene Expression; Molecular Sequence Data; Neuropeptide Y; Phylogeny; Receptors, Neuropeptide Y; Tissue Distribution; Tribolium; Journal Article; Research Support, Non-U.S. Gov't}},
language = {{eng}},
month = {{09}},
number = {{4}},
pages = {{83--578}},
publisher = {{Elsevier}},
series = {{Biochemical and Biophysical Research Communications}},
title = {{Identification of the Drosophila and Tribolium receptors for the recently discovered insect RYamide neuropeptides}},
url = {{http://dx.doi.org/10.1016/j.bbrc.2011.07.131}},
doi = {{10.1016/j.bbrc.2011.07.131}},
volume = {{412}},
year = {{2011}},
}