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The role of polyglucose in oxygen-dependent respiration by a new strain of Desulfovibrio salexigens

van Niel, Ed LU ; Pedro Gomes, Teresa M; Willems, Anne; Collins, Matthew D; Prins, Rudolf A and Gottschal, Jan C (1996) In FEMS Microbiology Ecology 21. p.243-253
Abstract
Desulfovibrio salexigens strain Mastl was isolated from the oxic/anoxic interface of a marine sediment. Growth under sulfate-reducing conditions was accompanied by polyglucose accumulation in the cell with every substrate tested. Highest polyglucose storage was found with glucose (0.8–1.0 g polyglucose (g protein)−1), but the growth rate with this substrate was very low (0.015 h−1). Anaerobically grown cells of strain Mastl exhibited immediate oxygen-dependent respiration. The endogenous oxygen reduction rate was proportional to the polyglucose content. The rate of aerobic respiration of pyruvate was also directly related to the polyglucose content indicating that this organism was only able to respire with oxygen as long as polyglucose... (More)
Desulfovibrio salexigens strain Mastl was isolated from the oxic/anoxic interface of a marine sediment. Growth under sulfate-reducing conditions was accompanied by polyglucose accumulation in the cell with every substrate tested. Highest polyglucose storage was found with glucose (0.8–1.0 g polyglucose (g protein)−1), but the growth rate with this substrate was very low (0.015 h−1). Anaerobically grown cells of strain Mastl exhibited immediate oxygen-dependent respiration. The endogenous oxygen reduction rate was proportional to the polyglucose content. The rate of aerobic respiration of pyruvate was also directly related to the polyglucose content indicating that this organism was only able to respire with oxygen as long as polyglucose was present. Maximum oxygen reduction rates were found at air saturating concentrations and were relatively low (3–50 nmol O2 min−1 (mg protein)−1). Catalase was constitutively present in anaerobically grown cells. When batch cultures were exposed to oxygen, growth ceased immediately and polyglucose was oxidized to acetate within 40–50 h. Like the oxygen reduction activity, the nitro blue tetrazolium (NBT)-reduction activity in these cells was proportional to the polyglucose content. Under anaerobic starvation conditions there was no correlation between the NBT-reduction activity and polyglucose concentration and polyglucose was degraded slowly within 240 h. The ecological significance of aerobic polyglucose consumption is discussed.
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author
publishing date
type
Contribution to journal
publication status
published
keywords
Desulfovibrio salexigens, sulfate reduction, polyglucose, oxygen-dependent respiration
in
FEMS Microbiology Ecology
volume
21
pages
11 pages
publisher
Elsevier
external identifiers
  • scopus:0030445283
ISSN
0168-6496
DOI
0.1016/S0168-6496(96)00060-8
language
English
LU publication?
no
id
c280c287-8531-43b0-9f1e-276392113661
date added to LUP
2016-09-06 13:07:14
date last changed
2017-06-26 18:07:45
@article{c280c287-8531-43b0-9f1e-276392113661,
  abstract     = {Desulfovibrio salexigens strain Mastl was isolated from the oxic/anoxic interface of a marine sediment. Growth under sulfate-reducing conditions was accompanied by polyglucose accumulation in the cell with every substrate tested. Highest polyglucose storage was found with glucose (0.8–1.0 g polyglucose (g protein)−1), but the growth rate with this substrate was very low (0.015 h−1). Anaerobically grown cells of strain Mastl exhibited immediate oxygen-dependent respiration. The endogenous oxygen reduction rate was proportional to the polyglucose content. The rate of aerobic respiration of pyruvate was also directly related to the polyglucose content indicating that this organism was only able to respire with oxygen as long as polyglucose was present. Maximum oxygen reduction rates were found at air saturating concentrations and were relatively low (3–50 nmol O2 min−1 (mg protein)−1). Catalase was constitutively present in anaerobically grown cells. When batch cultures were exposed to oxygen, growth ceased immediately and polyglucose was oxidized to acetate within 40–50 h. Like the oxygen reduction activity, the nitro blue tetrazolium (NBT)-reduction activity in these cells was proportional to the polyglucose content. Under anaerobic starvation conditions there was no correlation between the NBT-reduction activity and polyglucose concentration and polyglucose was degraded slowly within 240 h. The ecological significance of aerobic polyglucose consumption is discussed.<br/>},
  author       = {van Niel, Ed and Pedro Gomes, Teresa M and Willems, Anne and Collins, Matthew D and Prins, Rudolf A and Gottschal, Jan C},
  issn         = {0168-6496},
  keyword      = {Desulfovibrio salexigens,sulfate reduction,polyglucose,oxygen-dependent respiration},
  language     = {eng},
  pages        = {243--253},
  publisher    = {Elsevier},
  series       = {FEMS Microbiology Ecology},
  title        = {The role of polyglucose in oxygen-dependent respiration by a new strain of Desulfovibrio salexigens},
  url          = {http://dx.doi.org/0.1016/S0168-6496(96)00060-8},
  volume       = {21},
  year         = {1996},
}