The role of polyglucose in oxygen-dependent respiration by a new strain of Desulfovibrio salexigens
van Niel, Ed LU
; Pedro Gomes, Teresa M
; Willems, Anne
; Collins, Matthew D
; Prins, Rudolf A
and Gottschal, Jan C
(1996)
In FEMS Microbiology Ecology
21.
p.243-253
- Abstract
- Desulfovibrio salexigens strain Mastl was isolated from the oxic/anoxic interface of a marine sediment. Growth under sulfate-reducing conditions was accompanied by polyglucose accumulation in the cell with every substrate tested. Highest polyglucose storage was found with glucose (0.8–1.0 g polyglucose (g protein)−1), but the growth rate with this substrate was very low (0.015 h−1). Anaerobically grown cells of strain Mastl exhibited immediate oxygen-dependent respiration. The endogenous oxygen reduction rate was proportional to the polyglucose content. The rate of aerobic respiration of pyruvate was also directly related to the polyglucose content indicating that this organism was only able to respire with oxygen as long as polyglucose... (More)
- Desulfovibrio salexigens strain Mastl was isolated from the oxic/anoxic interface of a marine sediment. Growth under sulfate-reducing conditions was accompanied by polyglucose accumulation in the cell with every substrate tested. Highest polyglucose storage was found with glucose (0.8–1.0 g polyglucose (g protein)−1), but the growth rate with this substrate was very low (0.015 h−1). Anaerobically grown cells of strain Mastl exhibited immediate oxygen-dependent respiration. The endogenous oxygen reduction rate was proportional to the polyglucose content. The rate of aerobic respiration of pyruvate was also directly related to the polyglucose content indicating that this organism was only able to respire with oxygen as long as polyglucose was present. Maximum oxygen reduction rates were found at air saturating concentrations and were relatively low (3–50 nmol O2 min−1 (mg protein)−1). Catalase was constitutively present in anaerobically grown cells. When batch cultures were exposed to oxygen, growth ceased immediately and polyglucose was oxidized to acetate within 40–50 h. Like the oxygen reduction activity, the nitro blue tetrazolium (NBT)-reduction activity in these cells was proportional to the polyglucose content. Under anaerobic starvation conditions there was no correlation between the NBT-reduction activity and polyglucose concentration and polyglucose was degraded slowly within 240 h. The ecological significance of aerobic polyglucose consumption is discussed.
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Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/c280c287-8531-43b0-9f1e-276392113661
- author
- van Niel, Ed
LU
; Pedro Gomes, Teresa M
; Willems, Anne
; Collins, Matthew D
; Prins, Rudolf A
and Gottschal, Jan C
- publishing date
- 1996
- type
- Contribution to journal
- publication status
- published
- keywords
- Desulfovibrio salexigens, sulfate reduction, polyglucose, oxygen-dependent respiration
- in
- FEMS Microbiology Ecology
- volume
- 21
- pages
- 11 pages
- publisher
- Oxford University Press
- external identifiers
-
- scopus:0030445283
- ISSN
- 0168-6496
- DOI
- 10.1111/j.1574-6941.1996.tb00121.x
- language
- English
- LU publication?
- no
- id
- c280c287-8531-43b0-9f1e-276392113661
- date added to LUP
- 2016-09-06 13:07:14
- date last changed
- 2025-01-12 10:56:29
@article{c280c287-8531-43b0-9f1e-276392113661,
abstract = {{Desulfovibrio salexigens strain Mastl was isolated from the oxic/anoxic interface of a marine sediment. Growth under sulfate-reducing conditions was accompanied by polyglucose accumulation in the cell with every substrate tested. Highest polyglucose storage was found with glucose (0.8–1.0 g polyglucose (g protein)−1), but the growth rate with this substrate was very low (0.015 h−1). Anaerobically grown cells of strain Mastl exhibited immediate oxygen-dependent respiration. The endogenous oxygen reduction rate was proportional to the polyglucose content. The rate of aerobic respiration of pyruvate was also directly related to the polyglucose content indicating that this organism was only able to respire with oxygen as long as polyglucose was present. Maximum oxygen reduction rates were found at air saturating concentrations and were relatively low (3–50 nmol O2 min−1 (mg protein)−1). Catalase was constitutively present in anaerobically grown cells. When batch cultures were exposed to oxygen, growth ceased immediately and polyglucose was oxidized to acetate within 40–50 h. Like the oxygen reduction activity, the nitro blue tetrazolium (NBT)-reduction activity in these cells was proportional to the polyglucose content. Under anaerobic starvation conditions there was no correlation between the NBT-reduction activity and polyglucose concentration and polyglucose was degraded slowly within 240 h. The ecological significance of aerobic polyglucose consumption is discussed.<br/>}},
author = {{van Niel, Ed and Pedro Gomes, Teresa M and Willems, Anne and Collins, Matthew D and Prins, Rudolf A and Gottschal, Jan C}},
issn = {{0168-6496}},
keywords = {{Desulfovibrio salexigens; sulfate reduction; polyglucose; oxygen-dependent respiration}},
language = {{eng}},
pages = {{243--253}},
publisher = {{Oxford University Press}},
series = {{FEMS Microbiology Ecology}},
title = {{The role of polyglucose in oxygen-dependent respiration by a new strain of Desulfovibrio salexigens}},
url = {{https://lup.lub.lu.se/search/files/11875388/VanNieletalFEMSME1996.pdf}},
doi = {{10.1111/j.1574-6941.1996.tb00121.x}},
volume = {{21}},
year = {{1996}},
}