Xenotransplantation of human neural progenitor cells to the subretinal space of nonimmunosuppressed pigs.
Warfvinge, Karin LU
; Schwartz, Philip H
; Kiilgaard, Jens Folke
; la Cour, Morten
; Young, Michael J
; Scherfig, Erik
and Klassen, Henry
(2011)
In Journal of transplantation
2011(Article ID 948740).
- Abstract
- To investigate the feasibility of transplanting human neural progenitor cells (hNPCs) to the retina of nonimmunosuppressed pigs, cultured hNPCs were injected into the subretinal space of 5 adult pigs after laser burns were applied to promote donor cell integration. Postoperatively, the retinal vessels appeared normal without signs of exudation, bleeding, or subretinal elevation. Eyes were harvested at 10-28 days. H&E consistently showed mild retinal vasculitis, depigmentation of the RPE, and marked mononuclear cell infiltrate in the choroid adjacent to the site of transplantation. Human-specific antibodies revealed donor cells in the subretinal space at 10-13 days and smaller numbers within the retina on days 12 and 13, with evidence... (More)
- To investigate the feasibility of transplanting human neural progenitor cells (hNPCs) to the retina of nonimmunosuppressed pigs, cultured hNPCs were injected into the subretinal space of 5 adult pigs after laser burns were applied to promote donor cell integration. Postoperatively, the retinal vessels appeared normal without signs of exudation, bleeding, or subretinal elevation. Eyes were harvested at 10-28 days. H&E consistently showed mild retinal vasculitis, depigmentation of the RPE, and marked mononuclear cell infiltrate in the choroid adjacent to the site of transplantation. Human-specific antibodies revealed donor cells in the subretinal space at 10-13 days and smaller numbers within the retina on days 12 and 13, with evidence suggesting a limited degree of morphological integration; however, no cells remained at 4 weeks. The strong mononuclear cell reaction and loss of donor cells indicate that modulation of host immunity is likely necessary for prolonged xenograft survival in this model. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/2058553
- author
- Warfvinge, Karin
LU
; Schwartz, Philip H
; Kiilgaard, Jens Folke
; la Cour, Morten
; Young, Michael J
; Scherfig, Erik
and Klassen, Henry
- organization
- publishing date
- 2011
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of transplantation
- volume
- 2011
- issue
- Article ID 948740
- article number
- 948740
- publisher
- Hindawi Limited
- external identifiers
-
- pmid:21766011
- ISSN
- 2090-0015
- DOI
- 10.1155/2011/948740
- language
- English
- LU publication?
- yes
- id
- c283a698-75f4-4c72-8d1e-78d10b565ee4 (old id 2058553)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/21766011?dopt=Abstract
- date added to LUP
- 2016-04-01 13:25:37
- date last changed
- 2018-11-21 20:15:57
@article{c283a698-75f4-4c72-8d1e-78d10b565ee4,
abstract = {{To investigate the feasibility of transplanting human neural progenitor cells (hNPCs) to the retina of nonimmunosuppressed pigs, cultured hNPCs were injected into the subretinal space of 5 adult pigs after laser burns were applied to promote donor cell integration. Postoperatively, the retinal vessels appeared normal without signs of exudation, bleeding, or subretinal elevation. Eyes were harvested at 10-28 days. H&E consistently showed mild retinal vasculitis, depigmentation of the RPE, and marked mononuclear cell infiltrate in the choroid adjacent to the site of transplantation. Human-specific antibodies revealed donor cells in the subretinal space at 10-13 days and smaller numbers within the retina on days 12 and 13, with evidence suggesting a limited degree of morphological integration; however, no cells remained at 4 weeks. The strong mononuclear cell reaction and loss of donor cells indicate that modulation of host immunity is likely necessary for prolonged xenograft survival in this model.}},
author = {{Warfvinge, Karin and Schwartz, Philip H and Kiilgaard, Jens Folke and la Cour, Morten and Young, Michael J and Scherfig, Erik and Klassen, Henry}},
issn = {{2090-0015}},
language = {{eng}},
number = {{Article ID 948740}},
publisher = {{Hindawi Limited}},
series = {{Journal of transplantation}},
title = {{Xenotransplantation of human neural progenitor cells to the subretinal space of nonimmunosuppressed pigs.}},
url = {{https://lup.lub.lu.se/search/files/3363758/2220132.pdf}},
doi = {{10.1155/2011/948740}},
volume = {{2011}},
year = {{2011}},
}