The use of dispersed pancreatic islet cells in measurements of transmembrane transport
(1975) In Analytical Biochemistry 63(1). p.73-79- Abstract
Suspensions of dispersed islet cells were prepared by shaking collagenaseisolated pancreatic islets of ob ob-mice in Ca2+-free buffer. The dispersed cells exhibited a glucose uptake with stereospecificity for the d isomer and concentrated Rb+ about 30-fold from a medium containing 70 μm RbCl. These results compare well with previous observations on unbroken islets and indicate that the dispersion procedure does not cause serious damage to the plasma membranes of the β-cells. By double isotope labeling and centrifuging the incubated cells through oil, incubation times as short as only a few seconds can be used. The elimination of the extracellular tissue space and the short incubation times should facilitate the... (More)
Suspensions of dispersed islet cells were prepared by shaking collagenaseisolated pancreatic islets of ob ob-mice in Ca2+-free buffer. The dispersed cells exhibited a glucose uptake with stereospecificity for the d isomer and concentrated Rb+ about 30-fold from a medium containing 70 μm RbCl. These results compare well with previous observations on unbroken islets and indicate that the dispersion procedure does not cause serious damage to the plasma membranes of the β-cells. By double isotope labeling and centrifuging the incubated cells through oil, incubation times as short as only a few seconds can be used. The elimination of the extracellular tissue space and the short incubation times should facilitate the study of transport kinetics in the pancreatic islet cells.
(Less)
- author
- Lernmark, Åke LU ; Sehlin, Janove and Täljedal, Inge Bert
- publishing date
- 1975-01-01
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Analytical Biochemistry
- volume
- 63
- issue
- 1
- pages
- 7 pages
- publisher
- Elsevier
- external identifiers
-
- scopus:0016434168
- ISSN
- 0003-2697
- DOI
- 10.1016/0003-2697(75)90190-6
- language
- English
- LU publication?
- no
- id
- c28e80f5-5c1e-4888-8775-9da7f394ee09
- date added to LUP
- 2019-09-18 12:15:21
- date last changed
- 2021-01-03 04:49:56
@article{c28e80f5-5c1e-4888-8775-9da7f394ee09, abstract = {{<p>Suspensions of dispersed islet cells were prepared by shaking collagenaseisolated pancreatic islets of ob ob-mice in Ca<sup>2+</sup>-free buffer. The dispersed cells exhibited a glucose uptake with stereospecificity for the d isomer and concentrated Rb<sup>+</sup> about 30-fold from a medium containing 70 μm RbCl. These results compare well with previous observations on unbroken islets and indicate that the dispersion procedure does not cause serious damage to the plasma membranes of the β-cells. By double isotope labeling and centrifuging the incubated cells through oil, incubation times as short as only a few seconds can be used. The elimination of the extracellular tissue space and the short incubation times should facilitate the study of transport kinetics in the pancreatic islet cells.</p>}}, author = {{Lernmark, Åke and Sehlin, Janove and Täljedal, Inge Bert}}, issn = {{0003-2697}}, language = {{eng}}, month = {{01}}, number = {{1}}, pages = {{73--79}}, publisher = {{Elsevier}}, series = {{Analytical Biochemistry}}, title = {{The use of dispersed pancreatic islet cells in measurements of transmembrane transport}}, url = {{http://dx.doi.org/10.1016/0003-2697(75)90190-6}}, doi = {{10.1016/0003-2697(75)90190-6}}, volume = {{63}}, year = {{1975}}, }