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The use of dispersed pancreatic islet cells in measurements of transmembrane transport

Lernmark, Åke LU orcid ; Sehlin, Janove and Täljedal, Inge Bert (1975) In Analytical Biochemistry 63(1). p.73-79
Abstract

Suspensions of dispersed islet cells were prepared by shaking collagenaseisolated pancreatic islets of ob ob-mice in Ca2+-free buffer. The dispersed cells exhibited a glucose uptake with stereospecificity for the d isomer and concentrated Rb+ about 30-fold from a medium containing 70 μm RbCl. These results compare well with previous observations on unbroken islets and indicate that the dispersion procedure does not cause serious damage to the plasma membranes of the β-cells. By double isotope labeling and centrifuging the incubated cells through oil, incubation times as short as only a few seconds can be used. The elimination of the extracellular tissue space and the short incubation times should facilitate the... (More)

Suspensions of dispersed islet cells were prepared by shaking collagenaseisolated pancreatic islets of ob ob-mice in Ca2+-free buffer. The dispersed cells exhibited a glucose uptake with stereospecificity for the d isomer and concentrated Rb+ about 30-fold from a medium containing 70 μm RbCl. These results compare well with previous observations on unbroken islets and indicate that the dispersion procedure does not cause serious damage to the plasma membranes of the β-cells. By double isotope labeling and centrifuging the incubated cells through oil, incubation times as short as only a few seconds can be used. The elimination of the extracellular tissue space and the short incubation times should facilitate the study of transport kinetics in the pancreatic islet cells.

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author
; and
publishing date
type
Contribution to journal
publication status
published
subject
in
Analytical Biochemistry
volume
63
issue
1
pages
7 pages
publisher
Elsevier
external identifiers
  • scopus:0016434168
ISSN
0003-2697
DOI
10.1016/0003-2697(75)90190-6
language
English
LU publication?
no
id
c28e80f5-5c1e-4888-8775-9da7f394ee09
date added to LUP
2019-09-18 12:15:21
date last changed
2021-01-03 04:49:56
@article{c28e80f5-5c1e-4888-8775-9da7f394ee09,
  abstract     = {{<p>Suspensions of dispersed islet cells were prepared by shaking collagenaseisolated pancreatic islets of ob ob-mice in Ca<sup>2+</sup>-free buffer. The dispersed cells exhibited a glucose uptake with stereospecificity for the d isomer and concentrated Rb<sup>+</sup> about 30-fold from a medium containing 70 μm RbCl. These results compare well with previous observations on unbroken islets and indicate that the dispersion procedure does not cause serious damage to the plasma membranes of the β-cells. By double isotope labeling and centrifuging the incubated cells through oil, incubation times as short as only a few seconds can be used. The elimination of the extracellular tissue space and the short incubation times should facilitate the study of transport kinetics in the pancreatic islet cells.</p>}},
  author       = {{Lernmark, Åke and Sehlin, Janove and Täljedal, Inge Bert}},
  issn         = {{0003-2697}},
  language     = {{eng}},
  month        = {{01}},
  number       = {{1}},
  pages        = {{73--79}},
  publisher    = {{Elsevier}},
  series       = {{Analytical Biochemistry}},
  title        = {{The use of dispersed pancreatic islet cells in measurements of transmembrane transport}},
  url          = {{http://dx.doi.org/10.1016/0003-2697(75)90190-6}},
  doi          = {{10.1016/0003-2697(75)90190-6}},
  volume       = {{63}},
  year         = {{1975}},
}