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ST14A cells have properties of a medium-size spiny neuron

Ehrlich, Michelle E. ; Conti, Luciano ; Toselli, Mauro ; Taglietti, Luca ; Fiorillo, Edgardo ; Taglietti, Vanni ; Ivkovic, Sanja ; Guinea, Barbara ; Tranberg, Anna LU and Sipione, Simonetta , et al. (2001) In Experimental Neurology 167(2). p.215-226
Abstract

The ST14A cell line was previously derived from embryonic day 14 rat striatal primordia by retroviral transduction of the temperature-sensitive SV40 large T antigen. We showed that cell division and expression of nestin persists at 33°C, the permissive temperature, whereas cell division ceases, nestin expression decreases, and MAP2 expression increases at the nonpermissive temperature of 39°C. In this study, we further characterized the cells and found that they express other general and subtype-specific neuronal characteristics. ST14A cells express enolase and βIII-tubulin. Furthermore, they express the striatal marker DARPP-32, which is up-regulated upon differentiation of the cells by growth in serum-free medium. Stimulation with... (More)

The ST14A cell line was previously derived from embryonic day 14 rat striatal primordia by retroviral transduction of the temperature-sensitive SV40 large T antigen. We showed that cell division and expression of nestin persists at 33°C, the permissive temperature, whereas cell division ceases, nestin expression decreases, and MAP2 expression increases at the nonpermissive temperature of 39°C. In this study, we further characterized the cells and found that they express other general and subtype-specific neuronal characteristics. ST14A cells express enolase and βIII-tubulin. Furthermore, they express the striatal marker DARPP-32, which is up-regulated upon differentiation of the cells by growth in serum-free medium. Stimulation with dopamine, the D2-dopamine receptor agonist quinpirole, or the D1-dopamine receptor agonist SKF82958 results in phosphorylation of CREB. Treatment of the cells with a mixture of reagents which stimulate the MAPK and adenylyl cyclase pathways radically changes the morphology of the ST14A cells. The cells develop numerous neurite-like appearing processes which stain with βIII-tubulin. Moreover, under these conditions, intracellular injection of rectangular depolarizing current stimuli elicits overshooting action potentials with a relatively fast depolarization rate when starting from a strongly hyperpolarized membrane potential. Taken together, these data imply that the ST14A cell line displays some of the characteristics of a medium-size spiny neuron subtype and provides a new tool to elucidate the pathways and molecules involved in medium-size spiny neuron differentiation and disease.

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publishing date
type
Contribution to journal
publication status
published
subject
in
Experimental Neurology
volume
167
issue
2
pages
12 pages
publisher
Elsevier
external identifiers
  • scopus:0035125552
  • pmid:11161610
ISSN
0014-4886
DOI
10.1006/exnr.2000.7551
language
English
LU publication?
no
id
c29547de-e52b-4e2b-a206-092643bd24af
date added to LUP
2021-01-22 16:29:40
date last changed
2024-01-03 05:36:54
@article{c29547de-e52b-4e2b-a206-092643bd24af,
  abstract     = {{<p>The ST14A cell line was previously derived from embryonic day 14 rat striatal primordia by retroviral transduction of the temperature-sensitive SV40 large T antigen. We showed that cell division and expression of nestin persists at 33°C, the permissive temperature, whereas cell division ceases, nestin expression decreases, and MAP2 expression increases at the nonpermissive temperature of 39°C. In this study, we further characterized the cells and found that they express other general and subtype-specific neuronal characteristics. ST14A cells express enolase and βIII-tubulin. Furthermore, they express the striatal marker DARPP-32, which is up-regulated upon differentiation of the cells by growth in serum-free medium. Stimulation with dopamine, the D2-dopamine receptor agonist quinpirole, or the D1-dopamine receptor agonist SKF82958 results in phosphorylation of CREB. Treatment of the cells with a mixture of reagents which stimulate the MAPK and adenylyl cyclase pathways radically changes the morphology of the ST14A cells. The cells develop numerous neurite-like appearing processes which stain with βIII-tubulin. Moreover, under these conditions, intracellular injection of rectangular depolarizing current stimuli elicits overshooting action potentials with a relatively fast depolarization rate when starting from a strongly hyperpolarized membrane potential. Taken together, these data imply that the ST14A cell line displays some of the characteristics of a medium-size spiny neuron subtype and provides a new tool to elucidate the pathways and molecules involved in medium-size spiny neuron differentiation and disease.</p>}},
  author       = {{Ehrlich, Michelle E. and Conti, Luciano and Toselli, Mauro and Taglietti, Luca and Fiorillo, Edgardo and Taglietti, Vanni and Ivkovic, Sanja and Guinea, Barbara and Tranberg, Anna and Sipione, Simonetta and Rigamonti, Dorotea and Cattaneo, Elena}},
  issn         = {{0014-4886}},
  language     = {{eng}},
  number       = {{2}},
  pages        = {{215--226}},
  publisher    = {{Elsevier}},
  series       = {{Experimental Neurology}},
  title        = {{ST14A cells have properties of a medium-size spiny neuron}},
  url          = {{http://dx.doi.org/10.1006/exnr.2000.7551}},
  doi          = {{10.1006/exnr.2000.7551}},
  volume       = {{167}},
  year         = {{2001}},
}