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Methods for the preparation of chlorophyllide a: an intermediate of the chlorophyll biosynthetic pathway

Muller, A. H. ; Gough, S. P. ; Bollivar, D. W. ; Meldal, M. ; Willows, R. D. and Hansson, Mats LU (2011) In Analytical Biochemistry 419(2). p.271-276
Abstract
Chlorophyllide a is a metabolite late in the biosynthesis of chlorophylls and bacteriochlorophylls. Isolation procedures for chlorophyllide a from Rhodobacter capsulatus CB1200 and barley (Hordeum vulgare L.) are described and compared. R. capsulatus CB1200 is a double mutant in the bacteriochlorophyllide a biosynthetic pathway, and chlorophyllide a is excreted by the cells when grown in Tween 80-containing liquid medium. It was purified by liquid or solid phase extraction, yielding 7 mg of chlorophyllide a from 1 L of culture. In a second approach, intrinsic chlorophyllase activity was used to dephytylate chlorophyll in an acetonic preparation of leaves of wild-type or chlorophyll b-deficient barley. Purification was achieved by liquid... (More)
Chlorophyllide a is a metabolite late in the biosynthesis of chlorophylls and bacteriochlorophylls. Isolation procedures for chlorophyllide a from Rhodobacter capsulatus CB1200 and barley (Hordeum vulgare L.) are described and compared. R. capsulatus CB1200 is a double mutant in the bacteriochlorophyllide a biosynthetic pathway, and chlorophyllide a is excreted by the cells when grown in Tween 80-containing liquid medium. It was purified by liquid or solid phase extraction, yielding 7 mg of chlorophyllide a from 1 L of culture. In a second approach, intrinsic chlorophyllase activity was used to dephytylate chlorophyll in an acetonic preparation of leaves of wild-type or chlorophyll b-deficient barley. Purification was achieved by liquid phase extraction, yielding 14 mug of chlorophyllide a per gram of barley leaves. Chlorophyllide a was identified by thin layer chromatography, absorption spectroscopy, and mass spectrometry. (Less)
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author
; ; ; ; and
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Hordeum/metabolism, Culture Media/chemistry, Thin Layer, Chromatography, Chlorophyllides/*chemical synthesis/chemistry/isolation & purification, *Biosynthetic Pathways, Absorption, Biochemistry/*methods, Magnesium/metabolism, Polysorbates, Rhodobacter capsulatus/metabolism, Spectrum Analysis
in
Analytical Biochemistry
volume
419
issue
2
pages
271 - 276
publisher
Elsevier
external identifiers
  • scopus:80054056008
  • pmid:21925479
ISSN
1096-0309
DOI
10.1016/j.ab.2011.08.028
language
English
LU publication?
no
additional info
2
id
c2d2fb32-0de5-400f-8baf-4990920f5258 (old id 8001602)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/21925479
date added to LUP
2016-04-01 09:48:21
date last changed
2025-10-14 10:59:36
@article{c2d2fb32-0de5-400f-8baf-4990920f5258,
  abstract     = {{Chlorophyllide a is a metabolite late in the biosynthesis of chlorophylls and bacteriochlorophylls. Isolation procedures for chlorophyllide a from Rhodobacter capsulatus CB1200 and barley (Hordeum vulgare L.) are described and compared. R. capsulatus CB1200 is a double mutant in the bacteriochlorophyllide a biosynthetic pathway, and chlorophyllide a is excreted by the cells when grown in Tween 80-containing liquid medium. It was purified by liquid or solid phase extraction, yielding 7 mg of chlorophyllide a from 1 L of culture. In a second approach, intrinsic chlorophyllase activity was used to dephytylate chlorophyll in an acetonic preparation of leaves of wild-type or chlorophyll b-deficient barley. Purification was achieved by liquid phase extraction, yielding 14 mug of chlorophyllide a per gram of barley leaves. Chlorophyllide a was identified by thin layer chromatography, absorption spectroscopy, and mass spectrometry.}},
  author       = {{Muller, A. H. and Gough, S. P. and Bollivar, D. W. and Meldal, M. and Willows, R. D. and Hansson, Mats}},
  issn         = {{1096-0309}},
  keywords     = {{Hordeum/metabolism; Culture Media/chemistry; Thin Layer; Chromatography; Chlorophyllides/*chemical synthesis/chemistry/isolation & purification; *Biosynthetic Pathways; Absorption; Biochemistry/*methods; Magnesium/metabolism; Polysorbates; Rhodobacter capsulatus/metabolism; Spectrum Analysis}},
  language     = {{eng}},
  number       = {{2}},
  pages        = {{271--276}},
  publisher    = {{Elsevier}},
  series       = {{Analytical Biochemistry}},
  title        = {{Methods for the preparation of chlorophyllide a: an intermediate of the chlorophyll biosynthetic pathway}},
  url          = {{http://dx.doi.org/10.1016/j.ab.2011.08.028}},
  doi          = {{10.1016/j.ab.2011.08.028}},
  volume       = {{419}},
  year         = {{2011}},
}