Methods for the preparation of chlorophyllide a: an intermediate of the chlorophyll biosynthetic pathway
(2011) In Analytical Biochemistry 419(2). p.271-276- Abstract
- Chlorophyllide a is a metabolite late in the biosynthesis of chlorophylls and bacteriochlorophylls. Isolation procedures for chlorophyllide a from Rhodobacter capsulatus CB1200 and barley (Hordeum vulgare L.) are described and compared. R. capsulatus CB1200 is a double mutant in the bacteriochlorophyllide a biosynthetic pathway, and chlorophyllide a is excreted by the cells when grown in Tween 80-containing liquid medium. It was purified by liquid or solid phase extraction, yielding 7 mg of chlorophyllide a from 1 L of culture. In a second approach, intrinsic chlorophyllase activity was used to dephytylate chlorophyll in an acetonic preparation of leaves of wild-type or chlorophyll b-deficient barley. Purification was achieved by liquid... (More)
- Chlorophyllide a is a metabolite late in the biosynthesis of chlorophylls and bacteriochlorophylls. Isolation procedures for chlorophyllide a from Rhodobacter capsulatus CB1200 and barley (Hordeum vulgare L.) are described and compared. R. capsulatus CB1200 is a double mutant in the bacteriochlorophyllide a biosynthetic pathway, and chlorophyllide a is excreted by the cells when grown in Tween 80-containing liquid medium. It was purified by liquid or solid phase extraction, yielding 7 mg of chlorophyllide a from 1 L of culture. In a second approach, intrinsic chlorophyllase activity was used to dephytylate chlorophyll in an acetonic preparation of leaves of wild-type or chlorophyll b-deficient barley. Purification was achieved by liquid phase extraction, yielding 14 mug of chlorophyllide a per gram of barley leaves. Chlorophyllide a was identified by thin layer chromatography, absorption spectroscopy, and mass spectrometry. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/8001602
- author
- Muller, A. H. ; Gough, S. P. ; Bollivar, D. W. ; Meldal, M. ; Willows, R. D. and Hansson, Mats LU
- publishing date
- 2011
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Hordeum/metabolism, Culture Media/chemistry, Thin Layer, Chromatography, Chlorophyllides/*chemical synthesis/chemistry/isolation & purification, *Biosynthetic Pathways, Absorption, Biochemistry/*methods, Magnesium/metabolism, Polysorbates, Rhodobacter capsulatus/metabolism, Spectrum Analysis
- in
- Analytical Biochemistry
- volume
- 419
- issue
- 2
- pages
- 271 - 276
- publisher
- Elsevier
- external identifiers
-
- scopus:80054056008
- pmid:21925479
- ISSN
- 1096-0309
- DOI
- 10.1016/j.ab.2011.08.028
- language
- English
- LU publication?
- no
- additional info
- 2
- id
- c2d2fb32-0de5-400f-8baf-4990920f5258 (old id 8001602)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/21925479
- date added to LUP
- 2016-04-01 09:48:21
- date last changed
- 2022-01-25 08:53:16
@article{c2d2fb32-0de5-400f-8baf-4990920f5258, abstract = {{Chlorophyllide a is a metabolite late in the biosynthesis of chlorophylls and bacteriochlorophylls. Isolation procedures for chlorophyllide a from Rhodobacter capsulatus CB1200 and barley (Hordeum vulgare L.) are described and compared. R. capsulatus CB1200 is a double mutant in the bacteriochlorophyllide a biosynthetic pathway, and chlorophyllide a is excreted by the cells when grown in Tween 80-containing liquid medium. It was purified by liquid or solid phase extraction, yielding 7 mg of chlorophyllide a from 1 L of culture. In a second approach, intrinsic chlorophyllase activity was used to dephytylate chlorophyll in an acetonic preparation of leaves of wild-type or chlorophyll b-deficient barley. Purification was achieved by liquid phase extraction, yielding 14 mug of chlorophyllide a per gram of barley leaves. Chlorophyllide a was identified by thin layer chromatography, absorption spectroscopy, and mass spectrometry.}}, author = {{Muller, A. H. and Gough, S. P. and Bollivar, D. W. and Meldal, M. and Willows, R. D. and Hansson, Mats}}, issn = {{1096-0309}}, keywords = {{Hordeum/metabolism; Culture Media/chemistry; Thin Layer; Chromatography; Chlorophyllides/*chemical synthesis/chemistry/isolation & purification; *Biosynthetic Pathways; Absorption; Biochemistry/*methods; Magnesium/metabolism; Polysorbates; Rhodobacter capsulatus/metabolism; Spectrum Analysis}}, language = {{eng}}, number = {{2}}, pages = {{271--276}}, publisher = {{Elsevier}}, series = {{Analytical Biochemistry}}, title = {{Methods for the preparation of chlorophyllide a: an intermediate of the chlorophyll biosynthetic pathway}}, url = {{http://dx.doi.org/10.1016/j.ab.2011.08.028}}, doi = {{10.1016/j.ab.2011.08.028}}, volume = {{419}}, year = {{2011}}, }